A5035931021- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Ethics in Clinical Research
- Antibiotic Resistance in Bacteria
- Health Systems, Economic Evaluations, Quality of Life
- Biomedical Ethics and Regulation
- Long-Term Effects of COVID-19
- Antibiotic Use and Resistance
- Bone Metabolism and Diseases
- Viral Infections and Vectors
- COVID-19 and healthcare impacts
- COVID-19 epidemiological studies
- interferon and immune responses
- Antibiotics Pharmacokinetics and Efficacy
- Mosquito-borne diseases and control
- Autism Spectrum Disorder Research
- Virology and Viral Diseases
- Respiratory viral infections research
- Blood transfusion and management
- Genetics and Neurodevelopmental Disorders
- Pneumonia and Respiratory Infections
- Pharmaceutical Practices and Patient Outcomes
Hera General Hospital
2020-2025
Ministry of Health
2018-2025
Maternity and Children's Hospital
2021-2025
Health Affairs
2021-2024
Directorate of Health
2021-2024
Health Strategy and Policy Institute
2022-2024
Ministry of Health of the Russian Federation
2022-2024
Umm al-Qura University
2021-2022
Faculty of Public Health
2022
University of Arkansas for Medical Sciences
2022
The COVID-19 pandemic caused by SARS-CoV-2 affects all aspects of human life. Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly urgently needed to manage control the spread SARS-CoV-2. RT-qPCR based methods gold standard for detection. However, these require trained personnel, sophisticated infrastructure, a long turnaround time, thereby limiting their usefulness. Reverse transcription-loop-mediated isothermal amplification (RT-LAMP), one-step...
Abstract As the Coronavirus Disease 2019 (COVID-19), which is caused by novel SARS-CoV-2, continues to spread rapidly around world, there a need for well validated serological assays that allow detection of viral specific antibody responses in COVID-19 patients or recovered individuals. In this study, we established and used multiple indirect Enzyme Linked Immunosorbent Assay (ELISA)-based study response patients. order validate determined cut off values, sensitivity specificity using sera...
Abstract Monitoring SARS-CoV-2 spread and evolution through genome sequencing is essential in handling the COVID-19 pandemic. Here, we sequenced 892 genomes collected from patients Saudi Arabia March to August 2020. We show that two consecutive mutations (R203K/G204R) nucleocapsid (N) protein are associated with higher viral loads patients. Our comparative biochemical analysis reveals mutant N displays enhanced RNA binding differential interaction key host proteins. found increased of GSK3A...
The Coronavirus Disease 2019 (COVID-19), caused by SARS-CoV-2, continues to spread globally with significantly high morbidity and mortality rates. Antigen-specific responses are of unquestionable value for clinical management COVID-19 patients. Here, we investigated the kinetics IgM, IgG against spike (S) nucleoproteins (N) proteins their neutralizing capabilities in hospitalized patients different disease presentations (i.e., mild, moderate or severe), need intensive care units (ICU)...
Dengue fever is caused by four common serotypes of the dengue virus (DENV-1, DENV-2, DENV-3, and DENV-4). Patients infected with one serotype may develop lifelong serotype-specific protective immunity. However, they remain susceptible to reinfection other serotypes, often increasing risk severe forms dengue. This cross-sectional study investigates prevalence in patients who presented at Makkah hospitals between April 2023 May 2024. Data were collected from medical records Regional Laboratory...
The ethics committee has the responsibility to comply with rules and guidelines regarding oversight of all human research activities, particularly when study involves vulnerable people. It also role educating researchers on ethical issues, scientific truthfulness, preventing misconduct conflicts interest. In our we evaluate benchmark function local committees across country from point-of-view. We employed an online IRB-RAT survey measure perspectives investigators towards IRB functions...
Objective: To identify facilitators to routine medical checkups (RMCs) use among Saudi adults from the perspectives of primary care providers (PCPs). Methods: A qualitative phenomenological study design was conducted at five health centers (PHCs) in Makkah, Arabia. Data collected between December 2020 and February 2021 using semi-structured interviews with 19 PCPs. Descriptive statistics were performed characterize participants, a direct content analysis major themes. Results: number factors...
Thyroid cancer is the most prevalent endocrine among female population in Kingdom of Saudi Arabia (KSA) and ninth common male Arabia. Over past years, an increasing incidence thyroid has been reported However, etiology still not clear. Therefore, this study aimed to estimate mortality trends from 1990 2019. The current utilized Global Burden Disease Institute for Health Metrics Evaluation databases extract prevalence data Moreover, project utilizes (GBD) web-based tools visualize these data....
Abstract NFκB plays an important role in inflammation and bone remodelling. Tumour necrosis factor receptor associated 2 (TRAF2), a key component of signalling, has been identified as oncogene, but its the regulation breast cancer osteolytic metastasis remains unknown. Here, we report that stable overexpression TRAF2 parental osteotropic sub-clones human MDA-MB-231 (MDA-231) cells increased cell growth motility vitro , whereas knockdown was inhibitory. In vivo MDA-231-P enhanced tumour after...
The excessive inflammatory responses provoked by SARS-CoV-2 infection are critical factors affecting the severity and mortality of COVID-19. Previous work found that two adjacent co-occurring mutations R203K G204R (KR) on nucleocapsid (N) protein correlate with increased disease in COVID-19 patients. However, links host immune response remain unclear.Here, we grouped nasopharyngeal swab samples patients into cohorts based presence absence KR mutations. We performed transcriptome analysis...
To study the epidemiology of dengue incidence and understand dynamics transmission in Makkah, Kingdom Saudi Arabia (KSA), between 2017-2019.This is a cross-sectional study. Health demographic data was obtained for all confirmed cases KSA, years 2017-2019 from Vector-Borne Zoonotic Diseases Administration (VBZDA) Makkah Regional Laboratory, KSA. In addition, entomological about Aedes density VBZDA. Descriptive epidemiological methods were used to determine occurrence distribution...
(1) Background: Inappropriate use of antimicrobials and subsequently rise antimicrobial resistance (AMR) remains a major public health priority. Over-prescribing broad-spectrum antibiotics is one the main contributing factors for emergence AMR. We sought to describe prescribing trends among patients in hospitals Makkah hospitals. (2) Method: undertook point prevalence survey (PPS) six Makkah, Saudi Arabia, from January 2019 July 2019. The included all inpatients receiving on day PPS. Data...
Summary Monitoring SARS-CoV-2 spread and evolution through genome sequencing is essential in handling the COVID-19 pandemic. The availability of patient hospital records crucial for linking genomic sequence information to virus function during course infections. Here, we sequenced 892 genomes collected from patients Saudi Arabia March August 2020. From assembled sequences, estimate effective population size infection rate outline epidemiological dynamics import transmission events this...
In individuals with Duchenne muscular dystrophy (DMD), exon skipping treatment to restore a wild-type phenotype or correct the frame shift of mRNA transcript dystrophin (DMD) gene are mutation-specific. To explore molecular characterization DMD rearrangements and predict reading frame, we simultaneously screened all 79 exons 45 unrelated male patients using multiplex ligation-dependent probe amplification (MLPA) assay for deletion/duplication patterns. Multiplex PCR was used confirm single...
Abstract The Coronavirus Disease 2019 (COVID-19), caused by the novel SARS-CoV-2, continues to spread globally with significantly high morbidity and mortality rates. Immunological surrogate markers, in particular antigen-specific responses, are of unquestionable value for clinical management patients COVID-19. Here, we investigated kinetics IgM, IgG against spike (S) nucleoproteins (N) proteins their neutralizing capabilities hospitalized RT-PCR confirmed COVID-19 infection. Our data show...
Abstract Molecular diagnosis and surveillance of pathogens such as SARS‐CoV‐2 depend on nucleic acid isolation. Pandemics at the scale COVID‐19 can cause a global shortage proprietary commercial reagents BSL‐2 laboratories to safely perform testing. Therefore, alternative solutions are urgently needed address these challenges. An open‐source method, m agnetic‐nanoparticle‐ ided v iral R NA i solation from c ontagious s amples (MAVRICS), built upon readily available reagents, easily assembled...
Recent findings suggested several allosteric pockets on human aromatase that could be utilised for the development of new modulators able to inhibit this enzyme in a mechanism. Herein, we applied an integrated in-silico-based approach supported by in-vitro enzyme-based and cell-based validation assays select best leads target these binding sites from small library plant-derived natural products. Chrysin, apigenin, resveratrol were found inhibitors targeting enzyme's substrate access channel...
<h3>Objectives:</h3> To explore the perspectives of primary care providers (PCPs) on low use and barriers to routine checkups among Saudi adults. <h3>Methods:</h3> A qualitative phenomenological study design was used. Interviews were carried out between (December 2020 February 2021) with 19 PCPs working at 5 healthcare centers (PHCs) operated by Ministry Health (MOH) in Makkah, Arabia. Descriptive statistics performed characterize participants, a directed content analysis examine major...
Institutional review boards (IRBs) are formally designated to review, approve, and monitor biomedical research. They responsible for ensuring that researchers comply with the ethical guidelines concerning human research participants. Given IRBs might face different obstacles cause delays in their processes or conflicts investigators, this study aims report functions, roles, resources, process of Saudi Arabia.This was a cross-sectional self-reported survey conducted from March 2021 2022. The...
Objective: To determine whether individual or interactive single nucleotide polymorphisms (SNPs) may influence the development of autism spectrum disorder (ASD). Methods: DNA from buccal cells 212 participants (110 cases and 102 controls) were subjected to TaqMan genotyping HTR2A rs7997012, HTR2C rs6318, SLC6A4 rs3813034, ANKK1 rs1800497, BDNF rs6265 SNPs. The ASD symptoms severity assessed by DSM-IV criteria CARS scores. SNPStats software was used best model inheritance genotypic data....