A5075360124- Immune Cell Function and Interaction
- CAR-T cell therapy research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Respiratory viral infections research
- Cancer Research and Treatments
- Long-Term Effects of COVID-19
- Glioma Diagnosis and Treatment
- Immune cells in cancer
- Influenza Virus Research Studies
- Viral Infections and Immunology Research
- Extracellular vesicles in disease
- Infective Endocarditis Diagnosis and Management
- RNA Interference and Gene Delivery
- Chemokine receptors and signaling
- Monoclonal and Polyclonal Antibodies Research
Taibah University
2015-2025
King Abdullah International Medical Research Center
2020-2023
University of Florida
2022-2023
National Guard Health Affairs
2022
King Saud bin Abdulaziz University for Health Sciences
2022
King Saud University
2022
Harvard University
2018-2020
Brigham and Women's Hospital
2018-2020
University of Ottawa
2012-2018
Cairns Hospital
2017
Binding of programmed death ligand-1 (PD-L1) to cell protein-1 (PD1) leads cancer immune evasion via inhibition T function. One the defining characteristics glioblastoma, a universally fatal brain cancer, is its profound local and systemic immunosuppression. Glioblastoma has also been shown generate extracellular vesicles (EVs), which may play an important role in tumor progression. We thus hypothesized that glioblastoma EVs be mediators immunosuppression PD-L1 could role. show block...
Abstract MicroRNA deregulation is a consistent feature of glioblastoma, yet the biological effect each single gene generally modest, and therapeutically negligible. Here we describe module microRNAs, constituted by miR-124, miR-128 miR-137, which are co-expressed during neuronal differentiation simultaneously lost in gliomagenesis. Each one these miRs targets several transcriptional regulators, including oncogenic chromatin repressors EZH2, BMI1 LSD1, functionally interdependent involved...
Abstract As the Coronavirus Disease 2019 (COVID-19), which is caused by novel SARS-CoV-2, continues to spread rapidly around world, there a need for well validated serological assays that allow detection of viral specific antibody responses in COVID-19 patients or recovered individuals. In this study, we established and used multiple indirect Enzyme Linked Immunosorbent Assay (ELISA)-based study response patients. order validate determined cut off values, sensitivity specificity using sera...
Abstract Monitoring SARS-CoV-2 spread and evolution through genome sequencing is essential in handling the COVID-19 pandemic. Here, we sequenced 892 genomes collected from patients Saudi Arabia March to August 2020. We show that two consecutive mutations (R203K/G204R) nucleocapsid (N) protein are associated with higher viral loads patients. Our comparative biochemical analysis reveals mutant N displays enhanced RNA binding differential interaction key host proteins. found increased of GSK3A...
This study characterizes the ability of novel oncolytic rhabdoviruses (Maraba MG1) to boost natural killer (NK) cell activity. Our results demonstrate that MG1 activates NK cells via direct infection and maturation conventional dendritic cells. Using depletion ablation studies in vivo, we established both are required for efficacy. We further explored efficacy attenuated (nonreplicating MG1-UV(2min) single-cycle replicating MG1-Gless) demonstrated these viruses activate cells, although a...
Despite improvements in chemotherapy and radical surgical debulking, peritoneal carcinomatosis (PC) remains among the most common causes of death from abdominal cancers. Immunotherapies have been effective for selected solid malignancies, but their potential PC has little explored. Here, we report that intraperitoneal injection an infected cell vaccine (ICV), consisting autologous tumor cells ex vivo with oncolytic Maraba MG1 virus expressing IL12, promotes migration activated natural killer...
The Coronavirus Disease 2019 (COVID-19), caused by SARS-CoV-2, continues to spread globally with significantly high morbidity and mortality rates. Antigen-specific responses are of unquestionable value for clinical management COVID-19 patients. Here, we investigated the kinetics IgM, IgG against spike (S) nucleoproteins (N) proteins their neutralizing capabilities in hospitalized patients different disease presentations (i.e., mild, moderate or severe), need intensive care units (ICU)...
Abstract Aquaporins (AQPs) are ubiquitous channel proteins that play a critical role in the homeostasis of cellular environment by allowing transit water, chemicals, and ions. They can be found many different types cells organs, including lungs, eyes, brain, glands, blood vessels. By controlling osmotic water flux processes like cell growth, energy metabolism, migration, adhesion, proliferation, AQPs capable exerting their regulatory influence over wide range processes. Tumour varying...
Surgical removal of solid primary tumors is an essential component cancer treatment. Surgery-induced dysfunction in natural killer (NK) cells has been linked to the development metastases animal models and patients with cancer. We investigated activation NK using influenza vaccine perioperative period eradicate micrometastatic disease.Both B16lacZ 4T1 tumor immunocompetent mice were used assess vivo efficacy administration. In healthy human donors surgery patients, we assessed cell function...
Mitochondrial production of reactive oxygen species (ROS) affects many processes in health and disease. SPG7 assembles with AFG3L2 into the mAAA protease at inner membrane mitochondria, degrades damaged proteins, regulates synthesis mitochondrial ribosomes. is cleaved activated by upon assembly. A variant that replaces arginine 688 glutamine (Q688) associated several phenotypes, including toxicity chemotherapeutic agents, type 2 diabetes mellitus, (as reported here) coronary artery We...
Abstract The mode of action for oncolytic viruses (OVs) in cancer treatment is thought to depend on a direct initial cytotoxic effect against infected tumor cells and subsequent activation immune cell responses directed the neoplasm. To study both these effects mouse model glioblastoma (GBM), we employed murine GBM engineered constitutively express type I Herpes Simplex Virus (HSV1) HSV-1 receptor, nectin-1, allow more efficient infection replication by HSV (oHSV). These were further with...
According to the missing-self hypothesis, natural killer (NK) cells survey for target that lack MHC-I molecules. The Ly49 receptor family recognizes loss of and is critical educating NK cells, conferring ability eliminate transformed or infected cells. In this study, we evaluated their requirement in innate immune surveillance cancer using genetically manipulated mice with attenuated expression receptors (NKC(KD)) several models carcinoma metastasis. We found NKC(KD) exhibited uncontrolled...
The immune response to influenza virus infection comprises both innate and adaptive defenses. NK cells play an early role in the destruction of tumors virally-infected cells. express a variety inhibitory receptors, including those Ly49 family, which are functional homologs human killer-cell immunoglobulin-like receptors (KIR). Like KIR, inhibit cell-mediated lysis by binding major histocompatibility complex class I (MHC-I) molecules that expressed on normal During cell maturation,...
Abstract Considering the global trend to confine COVID‐19 pandemic by applying various preventive health measures, preprocedural mouth rinsing has been proposed mitigate transmission risk of SARS‐CoV‐2 in dental clinics. The study aimed investigate effect different rinses on salivary viral load patients. This was a single‐center, randomized, double‐blind, six‐parallel‐group, placebo‐controlled clinical trial that investigated four (1% povidone‐iodine, 1.5% hydrogen peroxide, 0.075%...
Ly49 receptors, which recognize "self" class I major histocompatibility complex (MHC-I) molecules, enable natural killer (NK) cells to detect loss of MHC-I expression on transformed and virally infected cells. The impact NK cell-mediated surveillance immunoediting breast cancer is still not fully understood. This work assesses the receptors tumor development in terms control driving immune-evading mutations. Genetically modified Ly49-deficient mice those lacking through antibody depletion...
Significance Finding that “innate” natural killer cells possessed T cell-like immune memory was an unprecedented discovery rocked our understanding of the divide between innate and adaptive immunity. In this report we demonstrate Ly49 receptors on cells, which engage class I antigen-presenting platform target are critical components cell memory. We also clinical potential memory, using it to mediate a cancer vaccine against melanoma. These findings offer insights into how might function what...