A5067893191- Advanced Proteomics Techniques and Applications
- Forensic Anthropology and Bioarchaeology Studies
- Metabolomics and Mass Spectrometry Studies
- Extracellular vesicles in disease
- Bone and Dental Protein Studies
- Mass Spectrometry Techniques and Applications
- Nanoplatforms for cancer theranostics
- RNA and protein synthesis mechanisms
- Forensic and Genetic Research
- Lipid Membrane Structure and Behavior
- Molecular Biology Techniques and Applications
- Genomics, phytochemicals, and oxidative stress
- Indigenous Studies and Ecology
- Nitrogen and Sulfur Effects on Brassica
- Analytical Chemistry and Chromatography
- Pharmacogenetics and Drug Metabolism
- Eicosanoids and Hypertension Pharmacology
- Glutathione Transferases and Polymorphisms
- Environmental Toxicology and Ecotoxicology
- MicroRNA in disease regulation
- Marine Biology and Environmental Chemistry
- Archaeology and ancient environmental studies
- Alzheimer's disease research and treatments
- Sex and Gender in Healthcare
- Cancer, Stress, Anesthesia, and Immune Response
University of Brighton
2016-2025
St Vincent's Hospital Sydney
2019-2023
Camden and Islington NHS Foundation Trust
2021
Hospital Universitario La Paz
2019
Brighton Hospital
2016
UPMC Hillman Cancer Center
2009-2014
University of Pittsburgh
2009-2014
Science Applications International Corporation (United States)
2007-2011
Duke Medical Center
2009
Duke University Hospital
2009
Significance The ability to assign biological sex human skeletal remains is a fundamental requirement in archaeology, paleoanthropology, and medico-legal sciences. While DNA sequencing can be used, it expensive, time-consuming, often fails due the poor quality of remaining DNA. An easier, more reliable, consistently applicable method needed. We present for determination using peptides retrieved from tooth enamel. Amelogenin an enamel-forming protein encoded by both chromosomes X Y, with...
Abstract Glioblastoma (GBM) is one of the most aggressive solid tumors for which treatment options and biomarkers are limited. Small extracellular vesicles (sEVs) produced by both GBM stromal cells central in inter-cellular communication that taking place tumor bulk. As sEVs accessible biofluids, recent reports have suggested contain valuable patient diagnosis follow-up. The aim current study was to describe protein content different cell lines patient-derived stem cells. Our results reveal...
Tooth enamel is the hardest, densest and most mineralized tissue in vertebrates.
Cells subjected to sustained high osmolarity almost universally respond by accumulating compatible organic osmolytes that, in contrast inorganic ions, are not deleterious even at intracellular concentrations. Their accumulation from the external environment known osmolyte transporters, such as four identified mammals, occurs only slowly response osmolarity, synthesis of new transporter proteins. Most cells, however, subject or varying and it is clear whether generally required normal...
Isothiocyanates (ITCs), such as phenethyl isothiocyanate (PEITC) and sulforaphane (SFN), are effective cancer chemopreventive compounds. It is believed that the major mechanism for preventive activity of ITCs through induction cell cycle arrest apoptosis. However, upstream molecular targets have been underexplored until recently. To identify proteins covalently modified by ITCs, human non-small lung A549 cells were treated with (14)C-PEITC (14)C-SFN, lysates extracted analysis 2-D gel...
Inflammation, characterized by the activation of both resident and infiltrated immune cells, is accompanied increased production oxidizing nitrating species. Nitrogen dioxide, proximal species formed under these conditions, reacts with unsaturated fatty acids to yield nitroalkene derivatives. These electrophilic products modulate protein function via post-translational modification susceptible nucleophilic amino acids. Nitroalkenes react Keap1 instigate Nrf2 signaling, activate heat shock...
Abstract Objectives This study tests, for the first time, applicability of a new method sex estimation utilizing enamel peptides on sample deciduous and permanent teeth at different stages mineralization, from nonadults unknown sex, including perinates. Materials methods A total 43 29 nonadult individuals aged 40 gestational weeks to 19 years old were analyzed. The included pairs fully mineralized just developing same individual. four archaeological sites in England: Piddington (1st–2nd...
Abstract Background Extracellular vesicles (EVs) are amongst the most promising candidates for developing blood-based biomarkers. However, patient sample availability is a key barrier to translational research whilst biobanks store samples of 1.5mL volume or less. To date, there no consensus on suitable method EV separation and current techniques frequently require large volumes biofluids, complicated technology, technical expertise, significant operating costs, which prevents their...
Mitochondrial production of reactive oxygen species (ROS) affects many processes in health and disease. SPG7 assembles with AFG3L2 into the mAAA protease at inner membrane mitochondria, degrades damaged proteins, regulates synthesis mitochondrial ribosomes. is cleaved activated by upon assembly. A variant that replaces arginine 688 glutamine (Q688) associated several phenotypes, including toxicity chemotherapeutic agents, type 2 diabetes mellitus, (as reported here) coronary artery We...
We recently developed a sensitive assay for 3′,5′-cAMP using high-performance liquid chromatography-tandem mass spectrometry. Using this assay, we investigated the release of from isolated, perfused rat kidneys. To our surprise, observed dominant chromatographic peak that was because an endogenous substance had same parent ion as and fragmented to daughter (adenine) 3′,5′-cAMP. However, retention time unknown approximately 2.9 min, compared with 6.3 min authentic hypothesized isomer The...
Glioblastoma (GB) is an aggressive type of tumour for which therapeutic options and biomarkers are limited. GB diagnosis mostly relies on symptomatic presentation the and, in turn, brain imaging invasive biopsy that can delay its diagnosis. Description easily accessible effective present biofluids would thus prove invaluable Extracellular vesicles (EVs) derived from both stromal cells essential to intercellular crosstalk bulk, circulating EVs have been described as a potential reservoir...
The efficiency of drug metabolism by a single enzyme can be measured as the fractional metabolic clearance which used measure whole body activity for that enzyme. Measurement multiple enzymes simultaneously is feasible using cocktail approach, however, analytical approach different assays probes cumbersome. A quantitative ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) based method rapid measurement six cytochrome P450 (CYP) probe drugs and their relevant...
Benign prostatic hyperplasia (BPH) is an age-related disease frequently associated with lower urinary tract symptoms (LUTS) that involves of both epithelial and stromal cells. Stromal fibrosis a distinctive feature BPH, but the exact mechanisms underlying this phenomenon are poorly understood.In current study, proteomics analyses were utilized to identify proteins altered in BPH compartment from patients symptomatic BPH. cells isolated histological nodules by laser capture microdissection...
Raw data obtained by ultra-high pressure liquid chromatography-mass spectrometry, and processed lipid compositional are presented alongside detailed methodology. Data were as bovine liver extract oxidizes, initiated 2,2'-Azobis(2-amidinopropane) dihydrochloride, at 0, 6 24 h post initiation. Lipid oxidation in the presence absence of some supplements with antioxidant properties was obtained. The used grape seed extract, pine bark milk thistle hawthorn turmeric extract.
Child labour is the most common form of child abuse in world today, with almost half workers employed hazardous industries. The large-scale employment children during rapid industrialisation late 18 th and early 19 centuries England well documented. During this period, removal pauper from workhouses cities to work as apprentices rural mills North was commonplace. Whilst experiences some these have been recorded historically, study provides first direct evidence their lives through...
Polycyclic aromatic hydrocarbons (PAHs) are environmental carcinogens implicated to underlie development of several types cancers. Cytochrome P450 (CYP) enzymes play key roles in the conversion PAHs highly potent carcinogens, namely diol epoxides. 7,12-Dimethylbenz(a)anthracene (DMBA), a PAH, is carcinogenic, where mouse models it known be responsible for initiating tumor formation many organs including mammary tissues, ovaries, and skin. Psychological stress, via release biochemical...
Dietary supplements containing plant extracts are often marketed as having antioxidant properties. Using complex lipid mixtures derived from cellular tissue, we perform a targeted lipidomic assessment of the activity five extract supplements. Formulations grape seed, milk thistle, pine bark, turmeric and hawthorn were evaluated. Overall, find evidence to suggest that all suppressed in vitro oxidation some degree. Suppression was most effective dominant classes, phosphatidylcholine (PC)...
The post-medieval period in Europe saw a dramatic increase metabolic bone disease related to vitamin D deficiency (VDD). Recent paleopathological work has utilized interglobular dentin (IGD) as proxy for poor status during development, while enamel peptide analysis allows the identification of chromosomal sex non-adult remains. Here we explore relationship between sex, presence IGD, and macroscopic markers VDD an industrial era assemblage from Northeast England.
The small-molecule drug, FTY720 (fingolimod), is a synthetic sphingosine 1-phosphate (S1P) analogue currently used to treat relapsing-remitting multiple sclerosis in both adults and children. can cross the blood-brain barrier (BBB) and, over time, accumulate lipid-rich areas of central nervous system (CNS) by incorporating into phospholipid membranes. has been shown enhance cell membrane fluidity, which modulate functions glial cells neuronal populations involved regulating behaviour....
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Abstract Trimethyl α‐amino derivatives of peptides (penta to deca) with a permanent positive charge on their groups were prepared by in vacuo reaction iodomethane and subjected matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS). Compared the unmodified peptide, signal intensity trimethyl derivative MALDI‐MS is increased at least an order magnitude. Similarly, octapeptide trimethylated ϵ‐amino group derived from solitary lysine residue B‐chain insulin also shows same...