A5072143605- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Endoplasmic Reticulum Stress and Disease
- Metabolism and Genetic Disorders
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- Autophagy in Disease and Therapy
- Heat shock proteins research
- Protein Structure and Dynamics
- thermodynamics and calorimetric analyses
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Genetic Neurodegenerative Diseases
- Genetics, Aging, and Longevity in Model Organisms
- Trace Elements in Health
- Advanced Electron Microscopy Techniques and Applications
- Bacterial Genetics and Biotechnology
- Nuclear Structure and Function
- Electrochemical sensors and biosensors
- Viral gastroenteritis research and epidemiology
- Biotin and Related Studies
- Cell death mechanisms and regulation
Max Planck Institute for Biology of Ageing
2018-2025
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2009-2018
University of Cologne
2009-2018
Institut für Forensische Genetik
2005
Ludwig-Maximilians-Universität München
2001
Kumamoto University
1998-2000
Nara Institute of Science and Technology
1999-2000
Osaka City University
1995
Prohibitins comprise an evolutionarily conserved and ubiquitously expressed family of membrane proteins with poorly described functions. Large assemblies PHB1 PHB2 subunits are localized in the inner mitochondria, but various roles other cellular compartments have also been proposed for both proteins. Here, we used conditional gene targeting murine Phb2 to define activities prohibitins. Our experiments restrict function prohibitins mitochondria identify processing dynamin-like GTPase OPA1,...
Mitochondria are dynamic organelles whose function depends on intramitochondrial phospholipid synthesis and the supply of membrane lipids from endoplasmic reticulum. How phospholipids transported to in-between mitochondrial membranes remained unclear. We identified Ups1, a yeast member conserved family intermembrane space proteins, as lipid transfer protein that can shuttle phosphatidic acid between membranes. Lipid required assembly Ups1 with Mdm35 allowed conversion cardiolipin in inner...
Lipid droplets (LDs) are conserved organelles for intracellular neutral lipid storage. Recent studies suggest that LDs function as direct sources autophagy, a central catabolic process in homeostasis and stress response. Here, we demonstrate dispensable membrane source but fulfill critical functions endoplasmic reticulum (ER) linked to autophagy regulation. In the absence of LDs, yeast cells display alterations their phospholipid composition fail buffer de novo fatty acid (FA) synthesis...
Abstract Coenzyme Q (or ubiquinone) is a redox-active lipid that serves as universal electron carrier in the mitochondrial respiratory chain and antioxidant plasma membrane limiting peroxidation ferroptosis. Mechanisms allowing cellular coenzyme distribution after synthesis within mitochondria are not understood. Here we identify cytosolic transfer protein STARD7 critical factor of intracellular transport suppressor Dual localization to intermembrane space cytosol upon cleavage by rhomboid...
Prohibitins comprise a remarkably conserved protein family in eukaryotic cells with proposed functions cell cycle progression, senescence, apoptosis, and the regulation of mitochondrial activities. Two prohibitin homologues, Phb1 Phb2, assemble into high molecular weight complex approximately 1.2 MDa inner membrane, but nuclear localization Phb2 also has been reported. Here, we have analyzed biogenesis structure Saccharomyces cerevisiae. Both subunits are targeted to mitochondria by...
The suppressor mutation, named sfhC21 , that allows Escherichia coli ftsH null mutant cells to survive was found be an allele of fabZ encoding R ‐3‐hydroxyacyl‐ACP dehydrase, involved in a key step fatty acid biosynthesis, and appears upregulate the dehydrase. ftsH1 (Ts) mutation increased amount lipopolysaccharide at 42°C. This accompanied by dramatic increase UDP‐3‐ O ‐( ‐3‐hydroxymyristoyl)‐ N ‐acetylglucosamine deacetylase [the lpxC ( envA ) gene product] committed lipid A biosynthesis....
ATP-binding cassette (ABC) adenosine triphosphatases actively transport a wide variety of compounds across biological membranes. Here, the ABC protein Mdl1 was identified as an intracellular peptide transporter localized in inner membrane yeast mitochondria. required for mitochondrial export peptides with molecular masses ∼2100 to 600 daltons generated by proteolysis inner-membrane proteins m-AAA protease matrix. Proteolysis i-AAA intermembrane space led release similar-sized independent...
The expression of heat shock genes in Escherichia coli is regulated by the antagonistic action transcriptional activator, σ 32 subunit RNA polymerase, and negative modulators. Modulators are DnaK chaperone system, which inactivates destabilizes , FtsH protease, largely responsible for degradation. A yet unproven hypothesis that degree sequestration modulators through binding to misfolded proteins determines level gene transcription. This was tested altering modulator concentration cells...
Escherichia coli FtsH is an ATP-dependent protease that belongs to the AAA protein family. The second region of homology (SRH) a highly conserved motif among family members and distinguishes these proteins in part from wider Walker-type ATPases. Despite its conservation across proteins, very little known concerning function SRH. To address this question, we introduced point mutations systematically into SRH studied activities mutant proteins. Highly amino acid residues within were found be...
Mitochondria exert critical functions in cellular lipid metabolism and promote the synthesis of major constituents membranes, such as phosphatidylethanolamine (PE) phosphatidylcholine. Here, we demonstrate that phosphatidylserine decarboxylase Psd1, located inner mitochondrial membrane, promotes PE via two pathways. First, Ups2–Mdm35 complexes (SLMO2–TRIAP1 humans) serve (PS)-specific transfer proteins intermembrane space, allowing formation by Psd1 membrane. Second, decarboxylates PS outer...
The generation of cellular energy depends on the coordinated assembly nuclear and mitochondrial-encoded proteins into multisubunit respiratory chain complexes in inner membrane mitochondria. Here, we describe identification a conserved metallopeptidase present intermembrane space, termed Atp23, which exerts dual activities during biogenesis F(1)F(O)-ATP synthase. On one hand, Atp23 serves as processing peptidase mediates maturation F(O)-subunit Atp6 after its insertion membrane. other hand...