A5021255614- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Ion channel regulation and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Cannabis and Cannabinoid Research
- Receptor Mechanisms and Signaling
- Genetics and Neurodevelopmental Disorders
- Pharmacological Receptor Mechanisms and Effects
- Epilepsy research and treatment
- Neuroscience of respiration and sleep
- Vagus Nerve Stimulation Research
- Alzheimer's disease research and treatments
- Neuroscience and Neural Engineering
- Parkinson's Disease Mechanisms and Treatments
- Sleep and Wakefulness Research
- Amyotrophic Lateral Sclerosis Research
- Neuropeptides and Animal Physiology
- Neural dynamics and brain function
- Tryptophan and brain disorders
- Mitochondrial Function and Pathology
- Neurological disorders and treatments
- Anesthesia and Neurotoxicity Research
- Stress Responses and Cortisol
University of Lisbon
2016-2025
Instituto de Medicina Molecular João Lobo Antunes
2015-2024
Hospital de Egas Moniz
2020-2024
Weatherford College
2023-2024
University of Minnesota System
2024
The University of Adelaide
2023
University of Minho
2023
University of Amsterdam
2023
Universidade de São Paulo
2013-2019
Institute of Pharmacology
2009-2016
Parkinson's disease (PD) is the most common representative of a group disorders known as synucleinopathies, in which misfolding and aggregation α-synuclein (a-syn) various brain regions major pathological hallmark. Indeed, motor symptoms PD are caused by heterogeneous degeneration neurons not only substantia nigra pars compacta but also other extrastriatal areas brain. In addition to well dysfunction patients, cognitive deficits memory impairment an important part disorder, probably due...
Abstract: The release of adenosine and ATP evoked by electrical field stimulation in rat hippocampal slices was investigated with the following two patterns stimulation: (1) a brief, high‐frequency burst (trains stimuli at 100 Hz for 50 ms applied every 2 s 1 min), to mimic long‐term potentiation (LTP) paradigm, (2) more prolonged (3 min) low‐frequency (5 Hz) train stimulation, depression (LTD) paradigm. greater whereas [ 3 H]adenosine slightly stimulation. To investigate source...
ATP analogs substituted in the γ-phosphorus (ATPγS, β,γ-imido-ATP, and β,γ-methylene-ATP) were used to probe involvement of P 2 receptors modulation synaptic transmission hippocampus, because their extracellular catabolism was virtually not detected CA1 slices. γ-substituted equipotent inhibit pyramid synapses (IC 50 17–22 μ m ). The inhibitory effect γ-phosphorus-substituted (30 ) modified by receptor antagonist suramin (100 ), inhibited 42–49% ecto-5′-nucleotidase inhibitor α,β-methylene...
Amyotrophic lateral sclerosis is characterized by a progressive degeneration of the corticospinal tract motor neurons. Growing evidence suggests that may begin at distal axon proceeding in dying-back pattern. It seemed therefore interest to investigate synaptic transmission neuromuscular junction (NMJ) pre- and symptomatic phases disease. Endplate potentials (EPPs), miniatures endplate (MEPPs) giant MEPPs (GMEPPs) were recorded from innervated diaphragm muscle fibers 4–6 12-15 weeks-old...
Brain-derived neurotrophic factor (BDNF) and its high-affinity full-length (FL) receptor, TrkB-FL, play a central role in the nervous system by providing trophic support to neurons regulating synaptic plasticity memory. TrkB BDNF signaling are impaired Alzheimer's disease (AD), neurodegenerative involving accumulation of amyloid-β (Aβ) peptide. We recently showed that Aβ leads decrease TrkB-FL receptor an increase truncated receptors unknown mechanism. In present study, we found (1)...
Engineering brain organoids from human induced pluripotent stem cells (hiPSCs) is a powerful tool for modeling development and neurological disorders. Rett syndrome (RTT), rare neurodevelopmental disorder, can greatly benefit this technology, since it affects multiple neuronal subtypes in forebrain sub-regions. We have established dorsal ventral control RTT patient-specific hiPSCs recapitulating 3D organization functional network complexity. Our data revealed premature of the deep-cortical...
Social isolation is a chronic mild stressor and significant risk factor for mental health disorders. Herein we explored the impact of social on depression- anxiety-like behaviours, as well spatial memory impairments, in middle-aged male mice compared to post-weaning mice. We aimed quantify correlate isolation-induced behaviour discrepancies with changes hippocampal glial cell reactivity pro-inflammatory cytokine levels. Post-weaning C57BL7/J6 were socially isolated 3-week period behavioural...
Both brain-derived neurotrophic factor (BDNF) and adenosine influence neuronal plasticity. We now investigated how influences the action of BDNF on synaptic transmission in CA1 area rat hippocampal slices. Alone, (20–100 ng/ml) did not significantly affect field EPSPs (fEPSPs). However, a 2 min pulse high-K + (10 m ) 46 before application (20 triggered an excitatory action, effect blocked by TrkB receptor inhibitor K252a (200 n ), A 2A antagonist ZM 241385 (50 protein kinase H-89 (1 μ )....
The effect of the stable adenosine analogue, 2-chloroadenosine (CADO), on currents elicited by iontophoretic application N-methyl-d-aspartate (NMDA) to pyramidal cells acutely dissociated from CA1 area rat hippocampus was studied using patch-clamp technique in whole-cell configuration. CADO (3-300 nM) reversibly inhibited NMDA receptor-mediated (maximal effect: 54.2 ± 6.6% decrease, EC50 = 10.3 nM). This prevented A1 receptor antagonist, 1,3-dipropyl-8-cyclopentylxanthine (DPCPX)(50 (100...
In the present work, we investigated action of adenosine originating from extracellular catabolism adenine nucleotides, in two preparations where synaptic transmission is modulated by both inhibitory A 1 and excitatory 2a ‐adenosine receptors, rat hippocampal Schaffer fibres/CA1 pyramid synapses innervated hemidiaphragm. Endogenous tonically inhibited transmission, since 0.5‐2 u ml −1 deaminase increased population spike amplitude (30 ± 4%) field post‐synaptic potential (f.e.p.s.p.) slope...