A5020102196- Cancer, Stress, Anesthesia, and Immune Response
- Stress Responses and Cortisol
- Lung Cancer Treatments and Mutations
- Estrogen and related hormone effects
- Gastric Cancer Management and Outcomes
- Dermatology and Skin Diseases
- Contact Dermatitis and Allergies
- Exercise and Physiological Responses
- Advanced Proteomics Techniques and Applications
- Cancer Cells and Metastasis
- Pancreatic function and diabetes
- Glioma Diagnosis and Treatment
- Radiopharmaceutical Chemistry and Applications
- Thermoregulation and physiological responses
- Immunotherapy and Immune Responses
- Head and Neck Cancer Studies
- Peptidase Inhibition and Analysis
- Anesthesia and Neurotoxicity Research
- Molecular Biology Techniques and Applications
- Tryptophan and brain disorders
- Allergic Rhinitis and Sensitization
- Brain Metastases and Treatment
- Immune Cell Function and Interaction
- Neuroendocrine regulation and behavior
- Cardiovascular Function and Risk Factors
University of Brighton
2016-2024
The University of Queensland
2018-2022
East Sussex County Council
2018-2021
University of Pittsburgh
2006-2016
UPMC Hillman Cancer Center
2005-2014
University of Kansas Medical Center
2014
Oregon Health & Science University
2014
University of Minnesota
2014
The University of Texas Health Science Center at San Antonio
2014
Massachusetts General Hospital
2014
Psychological stress increases the circulating levels of hormones cortisol and norepinephrine (NE). Chronic exposure to elevated has been linked a reduced response chemotherapy through induction DNA damage. We hypothesize that hormone signalling may induce damage production reactive oxygen species (ROS)/reactive nitrogen (RNS) interference in repair processes, promoting tumourigenesis. Breast cancer cell lines were incubated with physiological NE presence absence receptor antagonists...
The mechanisms by which stress hormones impact triple-negative breast cancer (TNBC) etiology and treatment are unclear. We have previously shown that hormones, cortisol, catecholamines induce rapid DNA damage repair in NIH 3T3 fibroblasts. This study investigates whether increase cells if this impacts drug efficacy.We first screened a panel of 39 cell lines for expression adrenergic glucocorticoid receptors examined alter cycle regulation vitro. A TNBC xenograft model was used to assess the...
Minimally invasive, reliable and low-cost in vivo biosensors that enable real-time detection monitoring of clinically relevant molecules biomarkers can significantly improve patient health care. Microneedle array (MNA)-based electrochemical sensors offer exciting prospects this respect, as they sample directly from the skin. However, their acceptability is dependent on developing a highly scalable cost-effective fabrication strategy. In work, we evaluated potential for poly(lactic...
The immune system plays an important role in controlling epithelial ovarian cancer (EOC). EOC is considered to be a "cold tumour," tumour that has not triggered strong response by the system. However, infiltrating lymphocytes (TILs) and expression of programmed cell death ligand (PD-L1) are used as prognostic indicators EOC. Immunotherapy such PD-(L)1 inhibitors have shown limited benefit Since affected behavioural stress beta-adrenergic signalling pathway, this study aimed explore impact...
Every winter, people with diabetes are at increased risk of severe influenza. At present, the mechanisms that cause this susceptibility unclear. Here, we show fluctuations in blood glucose levels common associated These data suggest glycemic stability could become a greater clinical priority for patients during outbreaks
Cognitive impairment is common in people diagnosed with breast cancer, but the molecular mechanisms that underlie maladaptive changes brain are unknown. The psychological stress of a cancer diagnosis certainly contributing factor. Here, we investigated alterations hippocampal proteome response to both and using label-free quantitative mass spectrometry techniques. An orthotopic syngeneic model triple-negative (TNBC) was established by injecting Py230 cells into mammary fat pads female...
Epinephrine and norepinephrine are produced during psychological stress can directly bind to cells induce DNA damage. These effects may have more long-lasting consequences such as mutations resulting in an increased potential for cellular transformation and/or tumor progression. This study examined the molecular of a chronic (24 h) vitro exposure these hormones on murine 3T3 cells. Long exposures dose-response experiments with or epinephrine induced significant increases damage treated...
Abstract Purpose: The EGF receptor (EGFR) and COX2 pathways are upregulated in head neck squamous cell carcinoma (HNSCC). Preclinical models indicate synergistic antitumor activity from dual blockade. We conducted a randomized, double-blind, placebo-controlled window trial of erlotinib, an EGFR inhibitor; erlotinib plus sulindac, nonselective COX versus placebo. Experimental Design: Patients with untreated, operable stage II-IVb HNSCC were randomized 5:5:3 to erlotinib–sulindac, or Tumor...
Esophageal adenocarcinoma (EAC) is associated with a dismal prognosis. The identification of cancer biomarkers can advance the possibility for early detection and better monitoring tumor progression and/or response to therapy. authors present results from development serum-based, 4-protein (biglycan, myeloperoxidase, annexin-A6, protein S100-A9) biomarker panel EAC.A vertically integrated, proteomics-based discovery approach was used identify candidate serum EAC. Liquid chromatography-tandem...
Investigational in vitro models that reflect the complexity of interaction between immune system and tumours are limited difficult to establish. Herein, we present a platform study tumour-immune using co-culture cancer spheroids activated cells. An algorithm was developed for analysis confocal images evaluate following quantitatively; cell infiltration, spheroid roundness growth. As proof concept, effect glucocorticoid stress hormone, cortisol tested on 66CL4 model. Results were comparable...
The canonical NOD-like receptor family pyrin domain containing 3 (NLRP3) pathway involves a priming step to induce pro-IL-1β followed by secondary signal such as K+ efflux activate inflammasome formation. This then leads the maturation of IL-1β and formation gasdermin D (GSDMD) pores that initiate pyroptosis mediate release. In contrast, primary human monocytes also engage an alternative in response toll-like (TLR) 4 activation, without need for signal. Data from monocyte-like cell line...
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Breast cancer is a highly heterogeneous disease, an observation that underscores the importance of elucidating conserved molecular characteristics, such as gene and protein expression, across breast cell types toward providing greater understanding context-specific features central to this disease. Motivated by goal defining biological themes subtypes, we conducted global proteomic analysis three lines, MCF7, SK-BR-3, MDA-MB-231, compared these model nontransformed mammary cells (MCF10A)....
The ability of stress to induce immune suppression is widely recognized, but the mechanisms underlying effects on adaptive system during tumor progression are not completely understood. To study effect
C57BL/6 mice, in contrast to BALB/c display minimal behavioral changes response environmental stressors and are considered relatively stress-resistant. We have shown that application of acute restraint prior chemical challenge enhanced cutaneous hypersensitivity (CHS) mice this is partially glucocorticoid dependent. Due strain differences the immune stressors, we hypothesized would not enhance CHS less stress-sensitive mice. sensitized challenged with contact sensitizer, 2,...
Abstract Treatment for chronic constipation in older people is challenging and the condition has a major impact on quality of life. A lack understanding about causes this hampered development effective treatments. 5-HT an important pro-kinetic agent colon. We examined whether alterations colonic signalling underlie age–related changes faecal output mice these were due to increase TNF-α. Components system (5-HT, 5-HIAA, SERT) TNF-α expression distal colon 3, 12, 18 24-month old water content...
Gaining insights into the molecular events that govern progression from melanoma in situ to advanced and understanding how local microenvironment at site influences this are two clinically pivotal aspects date largely unexplored. In an effort identify key regulators of crosstalk between cells melanoma-skin microenvironment, primary metastatic human were seeded skin organ cultures (SOCs) grown for weeks. Melanoma recovered SOCs by laser microdissection whole-cell tryptic digests analyzed...