A5085410951- Biochemical and Molecular Research
- Adenosine and Purinergic Signaling
- Estrogen and related hormone effects
- Breast Cancer Treatment Studies
- Cancer Genomics and Diagnostics
- Bioinformatics and Genomic Networks
- Folate and B Vitamins Research
- Gene expression and cancer classification
- Advanced Biosensing Techniques and Applications
- HER2/EGFR in Cancer Research
- Radiomics and Machine Learning in Medical Imaging
- Advanced Breast Cancer Therapies
- BRCA gene mutations in cancer
- IL-33, ST2, and ILC Pathways
- CAR-T cell therapy research
- Cytokine Signaling Pathways and Interactions
- Lymphoma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Breast Lesions and Carcinomas
- Cancer Immunotherapy and Biomarkers
- Influenza Virus Research Studies
- Epigenetics and DNA Methylation
- Eosinophilic Esophagitis
- Cancer Treatment and Pharmacology
- Respiratory viral infections research
UPMC Hillman Cancer Center
2012-2025
University of Pittsburgh
2011-2024
Public Health England
2019
Magee-Womens Research Institute
2015
Huntsman Cancer Institute
2014
University of Utah
2014
Magee-Womens Hospital
2013-2014
University of Pittsburgh Medical Center
2013-2014
Cancer Research Center
2014
University of Toronto
2013
Invasive lobular carcinoma (ILC) is a histologic subtype of breast cancer that frequently associated with favorable outcomes, as approximately 90% ILC express the estrogen receptor (ER). However, recent retrospective analyses suggest patients receiving adjuvant endocrine therapy may not benefit much invasive ductal carcinoma. On basis these observations, we characterized ER function and response in models. The ER-positive cell lines MDA MB 134VI (MM134) SUM44PE were used to examine...
The mechanisms by which stress hormones impact triple-negative breast cancer (TNBC) etiology and treatment are unclear. We have previously shown that hormones, cortisol, catecholamines induce rapid DNA damage repair in NIH 3T3 fibroblasts. This study investigates whether increase cells if this impacts drug efficacy.We first screened a panel of 39 cell lines for expression adrenergic glucocorticoid receptors examined alter cycle regulation vitro. A TNBC xenograft model was used to assess the...
The American Society of Clinical Oncology/College Pathologists (ASCO/CAP) guidelines recommend reporting hormone receptor test results in a semiquantitative manner. This study used 74 resected estrogen (ER)-positive invasive breast cancers to determine reproducibility scoring receptors using the H-score method. Four pathologists independently scored each slide. Agreement among observers was analyzed via Fleiss κ statistics on ER and progesterone (PR) categorical scores. Intraclass...
Abstract Purpose: To examine the effect of intratumor heterogeneity (ITH) on detection genes within gene expression panels (GEPs) and subsequent ability to predict prognostic risk. Experimental Design: Multiplexed barcoded RNA analysis was used measure 141 from five GEPs (Oncotype Dx, MammaPrint, PAM50, EndoPredict, Breast Cancer Index) in breast cancer tissue sections tumor-rich cores 71 estrogen receptor (ER)-positive node-negative tumors, which clinical Oncotype Dx testing previously...
The objective of this study was to examine the association between tobacco and alcohol dose type age onset pancreatic adenocarcinoma (PancCa).Prospective data from Pancreatic Cancer Collaborative Registry were used variables interest including: gender, race, birth country, educational status, family history PancCa, diabetes use. Statistical analysis included logistic linear regression, Cox proportional hazard time-to-event analysis.The median diagnosis for PancCa 66.3 years (95% confidence...
Influenza virus pandemics and seasonal epidemics have claimed countless lives. Recurrent zoonotic spillovers of influenza viruses with pandemic potential underscore the need for effective countermeasures. In this study, we show that pre-exposure prophylaxis broadly neutralizing antibody (bnAb) MEDI8852 is highly in protecting cynomolgus macaques from severe disease caused by aerosolized pathogenic avian H5N1 infection. Protection was dose–dependent yet independent Fc-mediated effector...
Breast Cancer Index (BCI) combines two independent biomarkers, HOXB13:IL17BR (H:I) and the 5-gene molecular grade index (MGI), that assess estrogen-mediated signalling tumor grade, respectively. BCI stratifies early-stage estrogen-receptor positive (ER+), lymph-node negative (LN-) breast cancer patients into three risk groups provides a continuous assessment of individual distant recurrence. Objectives current study were to validate in clinical case series compare prognostic utility...
The Flint Men's Health Study is an ongoing population-based study of African-American men designed to address questions related prostate cancer and urologic symptoms. initial phase the was conducted in 1996-1997 two stages: interviewer-administered survey followed by a clinical examination. response rate examination 52%. Thus, some data were missing for variables, diminishing generalizability results general population. This paper case demonstrating application multiple imputation important...
Decreased or absent progesterone receptor expression in invasive breast carcinoma is a marker for an adverse prognosis. As part of ongoing quality assurance study, this study evaluated the relationship between Oncotype DX recurrence score and immunohistochemical result within each Nottingham tumor grade 1074 cases which was available. In addition to statistically significant association categories (P < 0.001), inverse identified measured by modified H-score semiquantitation that independent...
Abstract p16 is a tumor suppressor encoded by the CDKN2A gene whose expression lost in approximately 50% of all human cancers. In its canonical role, inhibits G1–S-phase cell cycle progression through suppression cyclin-dependent kinases. Interestingly, also has roles metabolic reprogramming, and we previously published that loss promotes nucleotide synthesis via pentose phosphate pathway. However, broader impact p16/CDKN2A on other pathways potential therapeutic targets remains unexplored....
The ability of stress to induce immune suppression is widely recognized, but the mechanisms underlying effects on adaptive system during tumor progression are not completely understood. To study effect
No AccessJournal of UrologyCLINICAL UROLOGY: Original Articles1 May 2001THE NATURAL HISTORY OF LOWER URINARY TRACT SYMPTOMS IN BLACK AMERICAN MEN: RELATIONSHIPS WITH AGING, PROSTATE SIZE, FLOW RATE AND BOTHERSOMENESS JOHN T. WEI, DAVID SCHOTTENFELD, KRISTINE COOPER, JEREMY M. TAYLOR, GARY J. FAERBER, MARK A. VELARDE, ROBERT BREE, JAMES E. MONTIE, and KATHLEEN COONEY WEIJOHN WEI , SCHOTTENFELDDAVID SCHOTTENFELD COOPERKRISTINE COOPER TAYLORJEREMY TAYLOR FAERBERGARY FAERBER VELARDEMARK VELARDE...
We studied the impact of 96 hours formalin fixation on estrogen receptor (ER), progesterone (PR), and HER2 testing by comparing immunohistochemical results from core biopsy specimens fixed under current American Society Clinical Oncology (ASCO)/College Pathologists (CAP) guidelines with for corresponding resection samples hours. Samples enriched cases showing weak to moderate expression were included in study. Cases scored using ASCO/CAP guidelines. Of 47 cases, only 1 case (2%) showed a...
To compare the INFORM HER2 bright-field dual in situ hybridization (DISH) DNA probe cocktail assay with PathVysion fluorescence (FISH) on 103 invasive breast carcinomas a 2+ score immunohistochemistry (IHC).The cases were categorized as positive, equivocal, or negative for gene amplification using 2007 American Society of Clinical Oncology/College Pathologists (ASCO/CAP) HER2:CEP17 ratio criteria and also based mean copies/cell. The third criterion used 2 to categorize positive negative.The...
Abstract Objective: To assess the validity of multivariable models for predicting risk surgical site infection (SSI) after colorectal surgery based on routinely collected data in national surveillance networks. Design: Retrospective analysis performed 3 validation cohorts. Patients: Colorectal patients Switzerland, France, and England, 2007–2017. Methods: We determined calibration discrimination (ie, area under curve, AUC) COLA (contamination class, obesity, laparoscopy, American Society...
The combined gene protein assay (GPA) can simultaneously assess HER2 copy number and on a single slide using bright-field microscopy.GPA was compared with fluorescence in situ hybridization (FISH) 50 invasive breast carcinomas 2+ score immunohistochemistry (IHC).The cases were categorized into positive, equivocal, or negative for amplification the 2013 American Society of Clinical Oncology/College Pathologists criteria. This resulted 82% agreement (41 50) between FISH GPA. In addition, 25...
Highly pathogenic avian influenza A H5N1 viruses cause high mortality in humans and have pandemic potential. Effective vaccines treatments against this threat are urgently needed. Here, we refined our previously established model of lethal infection cynomolgus macaques. An inhaled aerosol virus dose 5.1 log
<h3>Background</h3> Our published preclinical data with T-regulatory cells depletion by E7777 supported synergistic effects anti-PD1 therapy in solid tumor xenograft models. This is a phase I trial primary objectives to investigate safety and establish II recommended dose of when combined pembrolizumab patients recurrent tumors. Secondary were assess efficacy term response rate (RR) impact on CD8 T-cells status. <h3>Methods</h3> given 4 levels (DL) (iv 3-12 mcg/kg day 1–3) 200 mg 1) 21-day...
Abstract Cancer predisposition syndromes (CPS) are underdiagnosed in the pediatric population, though diagnosis of a CPS has important implications for child and their family. often diagnosed by geneticists or oncologists with expertise following malignancy. This requires member care team, most commonly, treating oncologist to suspect refer patient assessment. An online survey was distributed members Children's Oncology Group elucidate current referral practices barriers patients suspected...