A5048429904- Malaria Research and Control
- Cellular transport and secretion
- Mosquito-borne diseases and control
- Antibiotic Resistance in Bacteria
- Tuberculosis Research and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Mycobacterium research and diagnosis
- Autophagy in Disease and Therapy
- Circular RNAs in diseases
- interferon and immune responses
- Extracellular vesicles in disease
- Lipid Membrane Structure and Behavior
- Pneumonia and Respiratory Infections
- Circadian rhythm and melatonin
- Bacterial Identification and Susceptibility Testing
- Complement system in diseases
- Viral Infections and Immunology Research
- MicroRNA in disease regulation
- Enterobacteriaceae and Cronobacter Research
- HIV Research and Treatment
- Calcium signaling and nucleotide metabolism
- Electrospun Nanofibers in Biomedical Applications
- Insect symbiosis and bacterial influences
- Cholesterol and Lipid Metabolism
- Polymer Surface Interaction Studies
King Abdullah University of Science and Technology
2017-2025
University of California, Irvine
2021
Sorbonne Paris Cité
2011-2014
Université Paris Cité
2009-2014
Institut Cochin
2009-2014
Délégation Paris 5
2013-2014
Inserm
2009-2013
Centre National de la Recherche Scientifique
2009-2011
Délégation Paris 7
2011
Hôpital Cochin
2009
Abstract Monitoring SARS-CoV-2 spread and evolution through genome sequencing is essential in handling the COVID-19 pandemic. Here, we sequenced 892 genomes collected from patients Saudi Arabia March to August 2020. We show that two consecutive mutations (R203K/G204R) nucleocapsid (N) protein are associated with higher viral loads patients. Our comparative biochemical analysis reveals mutant N displays enhanced RNA binding differential interaction key host proteins. found increased of GSK3A...
The final step during cell division is the separation of daughter cells, a process that requires coordinated delivery and assembly new membrane to cleavage furrow. While most eukaryotic cells replicate by binary fission, replication apicomplexan parasites involves daughters (merozoites/tachyzoites) within mother cell, using so-called Inner Membrane Complex (IMC) as scaffold. After de novo synthesis IMC biogenesis or segregation organelles, bud out invade host cells. Here, we demonstrate in...
Amino acid utilization is important for the growth of erythrocytic stages human malaria parasite Plasmodium falciparum, however molecular mechanism that permits survival during conditions limiting amino supply poorly understood. We provide data here suggesting an autophagy pathway functions in P. falciparum despite absence a typical lysosome digestion autophagosomes. It involves PfATG8, which has C-terminal glycine absolutely required association protein with starvation provoked increased...
Abstract Malaria parasites complete their intra-erythrocytic developmental cycle (IDC) in multiples of 24 h suggesting a circadian basis, but the mechanism controlling this periodicity is unknown. Combining vivo and vitro approaches utilizing rodent human malaria parasites, we reveal that: (i) 57% Plasmodium chabaudi genes exhibit daily rhythms transcription; (ii) 58% these lose transcriptional rhythmicity when IDC out-of-synchrony with host rhythms; (iii) 6% falciparum show expression under...
Abstract Malaria-associated pathogenesis such as parasite invasion, egress, host cell remodelling and antigenic variation requires concerted action by many proteins, but the molecular regulation is poorly understood. Here we have characterized an essential Plasmodium -specific Apicomplexan AP2 transcription factor in falciparum (PfAP2-P; pathogenesis) during blood-stage development with two peaks of expression. An inducible knockout gene function showed that PfAP2-P for trophozoite...
Klebsiella pneumoniae is a major bacterial and opportunistic human pathogen, increasingly recognized as healthcare burden globally. The convergence of resistance virulence in K. strains has led to the formation hypervirulent multidrug-resistant with dual risk, limiting treatment options. clones are known emerge locally spread Therefore, an understanding dynamics evolution emerging hospitals warranted prevent future outbreaks. In this study, we conducted in-depth genomic analysis on...
Theileria parasites are known to induce the transformation of host bovine leukocytes, involved in rapid proliferation, evasion from apoptotic mechanisms, and increased dissemination. In this study, we reveal involvement m
Electrospun scaffolds of poly(glycerol sebacate)/poly(ε-caprolactone) (PGS/PCL) have been used for engineered tissues due to their desirable thermal and mechanical properties as well tunable degradability.
Plasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesicular transport. However, PfRab5B is unique in lacking C-terminal geranyl-geranylation motif, while having N-terminal palmitoylation and myristoylation motifs. We show that the glycine required for vitro when an fragment possessing both acylation motifs fused GFP expressed transgenic P. parasites, chimeric protein localizes plasma membrane. Upon substitution modified by alanine staining becomes diffuse found...
Theileria annulata is an apicomplexan parasite that infects and transforms bovine macrophages disseminate throughout the animal causing a leukaemia-like disease called tropical theileriosis. Using deep RNAseq of T. annulata-infected B cells we identify set microRNAs induced by infection, whose expression diminishes upon loss hyper-disseminating phenotype virulent transformed macrophages. We describe how infection-induced upregulation miR-126-5p ablates JIP-2 to release cytosolic JNK...
Summary Monitoring SARS-CoV-2 spread and evolution through genome sequencing is essential in handling the COVID-19 pandemic. The availability of patient hospital records crucial for linking genomic sequence information to virus function during course infections. Here, we sequenced 892 genomes collected from patients Saudi Arabia March August 2020. From assembled sequences, estimate effective population size infection rate outline epidemiological dynamics import transmission events this...
MicroRNAs (miRNAs) are small noncoding RNAs that can play critical roles in regulating various cellular processes, including during many parasitic infections. Here, we report a regulatory role for miR-34c-3p cAMP-independent regulation of host cell protein kinase A (PKA) activity Theileria annulata-infected bovine leukocytes. We identified prkar2b (cAMP-dependent type II-beta subunit) as novel target gene and demonstrate how infection-induced upregulation repressed PRKAR2B expression to...
Abstract Background information The pathology causing stages of the human malaria parasite Plasmodium falciparum reside within red blood cells that are devoid any regulated transport system. parasite, therefore, is entirely responsible for mediating vesicular itself and in infected erythrocyte cytoplasm, it does so part via its family 11 Rab GTPases. Putative functions have been ascribed to Rabs due their homology with yeast, particularly Saccharomyces has an equivalent number rab/ypt genes...
Mycobacterium kansasii is an important opportunistic pathogen of humans and has a close phylogenetic relationship with tuberculosis. Seven subtypes (I-VII) have been identified using molecular biology approaches, which subtype I the most frequent causative agent human disease. To investigate genotypes pathogenic components M. kansasii, we sequenced compared complete base-perfect genomes different subtypes. Our findings support proposition that "subtypes" I-VI, whose assemblies are currently...
The Saudi government requires that all pilgrims receive a quadrivalent meningococcal vaccine at least 10 days before the Hajj. We conducted study to determine uptake of and antibiotic use. also investigated risk factors carriage Neisseria meningitidis pathogenic serogroups A, C, W Y.A cross-sectional oropharyngeal survey was in 2973 Hajj September 2017. A real-time polymerase chain reaction (rt-PCR) assay used identify N. from swabs. questionnaire potential for meningitidis.Two thousand two...
Studies have shown that variants in bedaquiline-resistance genes can occur isolates from bedaquiline-naive patients. We assessed the prevalence of all bedaquiline-candidate-resistance patients, investigated association between these and lineage, effect on phenotype.
Malaria pathogenicity results from the parasite's ability to invade, multiply within and then egress host red blood cell (RBC). Infected RBCs are remodeled, expressing antigenic variant proteins (such as PfEMP1, coded by var gene family) for immune evasion survival. These processes require concerted actions of many proteins, but molecular regulation is poorly understood. We have characterized an essential Plasmodium specific Apicomplexan AP2 (ApiAP2) transcription factor in falciparum...
Theileria annulata is a tick-transmitted apicomplexan parasite that infects and transforms bovine leukocytes into disseminating tumours cause disease called tropical theileriosis. Using comparative transcriptomics we identified genes transcriptionally perturbed during Theileria-induced leukocyte transformation. Dataset comparisons highlighted small set of associated with Theileria-transformed dissemination. The roles Granzyme A (GZMA) RAS guanyl-releasing protein 1 (RASGRP1) were verified by...
Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI
Malaria parasites complete their intra-erythrocytic developmental cycle (IDC) in multiples of 24 hours (depending on the species), suggesting a circadian basis to asexual cell cycle, but mechanism controlling this periodicity is unknown. Combining vivo and vitro approaches using rodent human malaria parasites, we reveal that: (i) 57% Plasmodium chabaudi genes exhibit h “circadian” transcription; (ii) 58% these lose transcriptional rhythmicity when IDC out-of-synchrony with host rhythms;...
Summary An aberrant innate immune system caused by the beta coronavirus SARS-CoV-2 is a characteristic manifestation of severe disease 2019 (COVID-19). Here, we performed proteome profiling nasopharyngeal (NP) swabs from 273 hospitalized patients with mild and COVID-19 symptoms, including non-survivors. We identified depletion in STAT1-mediated type I interferon response, retinol metabolism NRF2 antioxidant that are associated severity our patient demography. found dysregulation...