A5011164777- Vector-borne infectious diseases
- Toxin Mechanisms and Immunotoxins
- Heat shock proteins research
- Circular RNAs in diseases
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Antimicrobial Peptides and Activities
- MicroRNA in disease regulation
- Invertebrate Immune Response Mechanisms
- Pancreatic and Hepatic Oncology Research
- Viral Infections and Vectors
- T-cell and B-cell Immunology
- Trypanosoma species research and implications
- RNA Interference and Gene Delivery
- Toxoplasma gondii Research Studies
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Thermoregulation and physiological responses
- TGF-β signaling in diseases
- Congenital heart defects research
- RNA and protein synthesis mechanisms
King Abdullah University of Science and Technology
2017-2025
University of Basel
2025
de Duve Institute
2021-2024
Institut Cochin
2016-2023
Université Paris Cité
2015-2023
Inserm
2015-2023
Centre National de la Recherche Scientifique
2016-2023
UCLouvain
2021-2023
Délégation Paris 5
2015-2021
Sorbonne Paris Cité
2015-2021
Abstract Background MIRAGE syndrome is a rare multisystem disorder characterized by myelodysplasia, infection, growth restriction, adrenal hypoplasia, genital phenotypes, and enteropathy, resulting from mutations in the SAMD9 gene. This notably rare, with fewer than 60 documented cases globally. We present unique case of an 8-month-old boy exhibiting previously unreported clinical manifestation pyloric stenosis, contributing to expanding phenotypic spectrum disorder. Case presentation An who...
Theileria parasites are known to induce the transformation of host bovine leukocytes, involved in rapid proliferation, evasion from apoptotic mechanisms, and increased dissemination. In this study, we reveal involvement m
Background Pancreatic ductal adenocarcinoma (PDAC) arises from distinct cellular origins, yet the extent to which DNA methylation patterns normal pancreatic cells are preserved in tumor remains unclear. Identifying cell-of-origin signatures may enhance PDAC classification and therapeutic stratification. Objective To determine whether acinar retained cell lines develop a robust classifier for distinguishing origins. Design We performed profiling using Illumina Infinium Mouse MethylationEPIC...
Transforming growth factor beta (TGF-β) is a pleiotropic cytokine known to regulate cell growth, differentiation, and motility potent modulator of immune function. TGF-β consequently plays central role in carcinogenesis, dampened TGF-β2 response by Theileria annulata-infected monocytes/macrophages underpins disease resistance tropical theileriosis. Here, we show that concomitant with the loss production, there ablated expression COX2 EP4, which leads drop cyclic AMP (cAMP) levels and,...
Abstract Theileria -infected macrophages display many features of cancer cells such as heightened invasive capacity; however, the tumor-like phenotype is reversible by killing parasite. Moreover, virulent can be attenuated multiple in vitro passages and so provide a powerful model to elucidate mechanisms related transformed macrophage virulence. Here, we demonstrate that two independent -transformed cell lines Grb2 expression down-regulated concomitant with loss tumor Using peptidimer-c...
Theileria annulata is an apicomplexan parasite that infects and transforms bovine macrophages disseminate throughout the animal causing a leukaemia-like disease called tropical theileriosis. Using deep RNAseq of T. annulata-infected B cells we identify set microRNAs induced by infection, whose expression diminishes upon loss hyper-disseminating phenotype virulent transformed macrophages. We describe how infection-induced upregulation miR-126-5p ablates JIP-2 to release cytosolic JNK...
We previously reported that Staphylococcus aureus Cowan I (SAC) is a potent stimulant of IgM rheumatoid factor (RF) production by normal adult peripheral blood mononuclear cells. In the current study, we compared capacity cells and cord to produce RF. Although both populations consistently produced RF in response SAC, quantity (128 +/- 18 ng/ml, mean SEM) greatly exceeded (37 5 p less than 0.0005) even though demonstrated comparable total responses. Remarkably, 16.7% SAC showed activity only...
MicroRNAs (miRNAs) are small noncoding RNAs that can play critical roles in regulating various cellular processes, including during many parasitic infections. Here, we report a regulatory role for miR-34c-3p cAMP-independent regulation of host cell protein kinase A (PKA) activity Theileria annulata-infected bovine leukocytes. We identified prkar2b (cAMP-dependent type II-beta subunit) as novel target gene and demonstrate how infection-induced upregulation repressed PRKAR2B expression to...
These studies demonstrate that Staphylococcus aureus Cowan I (SAC), a protein A-positive Staphylococcal strain, is potent and consistent inducer of IgM rheumatoid factor production by normal human peripheral blood mononuclear cells. The frequency magnitude this response greatly exceeded parallel cultures stimulated with pokeweed mitogen or the A-negative S. Wood although all three agents induced similar amount total IgM. Cell fractionation indicated SAC-induced T cell-dependent. striking...
Abstract Theileria are tick‐transmitted parasites that cause often fatal leuko‐proliferative diseases in cattle called tropical theileriosis ( T. annulata ) and East Coast fever parva ). However, upon treatment with anti‐theilerial drug‐transformed leukocytes die of apoptosis indicating ‐induced transformation is reversible making infected a powerful example how intracellular interact their hosts. ‐transformed disseminate throughout causing cancer‐like disease here, we discuss cytokines,...
Intracellular pathogens have evolved intricate mechanisms to subvert host cell signaling pathways and ensure their own propagation. A lineage of the protozoan parasite genus Theileria infects bovine leukocytes induces uncontrolled proliferation causing a leukemia-like disease. Given importance E2F transcription factors in mammalian cycle regulation, we investigated role Theileria-induced proliferation. Using comparative genomics surface plasmon resonance, identified parasite-derived peptides...
Theileria annulata is a tick-transmitted apicomplexan parasite that infects and transforms bovine leukocytes into disseminating tumours cause disease called tropical theileriosis. Using comparative transcriptomics we identified genes transcriptionally perturbed during Theileria-induced leukocyte transformation. Dataset comparisons highlighted small set of associated with Theileria-transformed dissemination. The roles Granzyme A (GZMA) RAS guanyl-releasing protein 1 (RASGRP1) were verified by...
Constitutive c-Jun N-terminal kinase (JNK) activity characterizes bovine T and B cells infected with Theileria parva, macrophages annulata. Here, we show that T. annulata infection of manipulates JNK activation by recruiting JNK2 not JNK1 to the parasite surface, whereas is found predominantly in host cell nucleus. At parasite's forms a complex p104, GPI-(GlycosylPhosphatidylInositol)-anchor plasma membrane protein. Sequestration depended on Protein Kinase-A (PKA)-mediated phosphorylation...
Grapevines were surveyed for the presence of virus and virus‐like diseases in main viticultural areas Lebanon (Bekaa valley, Mount Lebanon, South North Lebanon). Symptoms rugose wood observed vines ofall cultivars surveyed, whereas leafroll was only some vineyards Bekaa valley and, to a lesser extent, on cvs Tfaifihi, Cinsaut Cardinal. fanleaf phytoplasma‐induced yellows also with low frequency wine‐grape cultivars. ELISA tests showed that 53% 1536 Vitis vinifera individually checked...
Theileria annulata infects bovine leukocytes, transforming them into invasive, cancer-like cells that cause the widespread disease called tropical theileriosis. We report in Theileria-transformed leukocytes hexokinase-2 (HK2) binds to B cell lymphoma-2-associated death promoter (BAD) only when serine (S) 155 BAD is phosphorylated. show HK2 recruitment abolished by a cell-penetrating peptide acts as nonphosphorylatable substrate inhibits endogenous S155 phosphorylation, leading complex...
Abstract Theileria is a unique apicomplexan parasite capable of transforming its host cell into disseminating tumour. Constitutive JNK activity characterizes bovine T and B cells infected with T. parva , macrophages annulata. Here, we show that annulata manipulates activation by recruiting JNK2, not JNK1, to the surface, whereas JNK1 found predominantly in nucleus. In silico analysis identified 3 potential JNK-binding motifs previously characterized GPI-anchored macroschizont surface protein...