A5058559600- Malaria Research and Control
- NMR spectroscopy and applications
- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Natural product bioactivities and synthesis
- Metabolomics and Mass Spectrometry Studies
- Free Radicals and Antioxidants
- Molecular spectroscopy and chirality
- Electrochemical Analysis and Applications
- Electron Spin Resonance Studies
- Biological Activity of Diterpenoids and Biflavonoids
- Enzyme Structure and Function
- Computational Drug Discovery Methods
- Protein Structure and Dynamics
- Complement system in diseases
- HIV Research and Treatment
- Trypanosoma species research and implications
- Alkaloids: synthesis and pharmacology
King Abdullah University of Science and Technology
2023
National Chemical Laboratory
2018
University of Geneva
2010-2013
Abstract Malaria-associated pathogenesis such as parasite invasion, egress, host cell remodelling and antigenic variation requires concerted action by many proteins, but the molecular regulation is poorly understood. Here we have characterized an essential Plasmodium -specific Apicomplexan AP2 transcription factor in falciparum (PfAP2-P; pathogenesis) during blood-stage development with two peaks of expression. An inducible knockout gene function showed that PfAP2-P for trophozoite...
Abstract Glucose is an essential nutrient for Plasmodium falciparum and robust glycolytic activity indicative of viable parasites. Using NMR spectroscopy, we show that P. infected erythrocytes consume ~20 times more glucose, trophozoites metabolize ~6 glucose than ring stage The activity, hence parasite viability, can be measured within a period 2 h to 5 h, using this method. This facilitates antimalarial bioactivity screening on trophozoite parasites, exclusively. We demonstrate potent...
(13)C-Cholesterol was produced with high efficiency by a genetically engineered yeast strain. The method produces ∼ 1 mg of cholesterol per gram glucose using 100 ml culture medium. Uniform 94% enrichment where the most abundant product is fully enriched isotopomer (u-(13)C(27)) obtained (u-(13)C(6), 99%) High very important for relaxation experiments, but NMR applications carbon-carbon couplings are measured, this problematic. A good compromise between sensitivity and cost consists in...
Abstract A new indole alkaloid, calanthumindole ( 4 ), and three known biflavonoids, amentoflavone, sequoiaflavone, podoscarpusflavone B, were isolated from Campylospermum calanthum (Ochnaceae). Calanthumindole is a alkaloid of the serotobenine family characterized by presence CC bond between atoms C(7′) C(8′) furan ring. This first compound to have fully unsaturated ring among members this family. The combination NMR DFT allowed determination comparison 3D structures relevant...
NMR titrations can be used to simultaneously determine the pKa's of mixtures organic acids and bases provided chemical shifts are sensitive their change protonation states. Carbons in principle better probes than protons because typically more changes electronic densities functional groups they bound to. They also less prone overlap, do not suffer from second-order effects solvent conditions. Recording HSQC spectra satisfactorily reduces lack sensitivity carbon-detected experiments signal...