A5015977998- Heat shock proteins research
- Malaria Research and Control
- Endoplasmic Reticulum Stress and Disease
- Vector-borne infectious diseases
- Protein Structure and Dynamics
- Poxvirus research and outbreaks
- Computational Drug Discovery Methods
- thermodynamics and calorimetric analyses
- Trypanosoma species research and implications
- Mosquito-borne diseases and control
- Parasitic Infections and Diagnostics
- Toxin Mechanisms and Immunotoxins
- Research on Leishmaniasis Studies
- Enzyme Structure and Function
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Bacteriophages and microbial interactions
- Cellular transport and secretion
- Salmonella and Campylobacter epidemiology
- Erythrocyte Function and Pathophysiology
- SARS-CoV-2 and COVID-19 Research
- Bacillus and Francisella bacterial research
- Rabies epidemiology and control
- Amoebic Infections and Treatments
- Veterinary medicine and infectious diseases
Indian Institute of Science Bangalore
2016-2025
National Institute of Animal Nutrition and Physiology
2023
University of California, Davis
2019
Indian Academy of Sciences
2014
Yale University
1992-1997
University of Massachusetts Amherst
1993
Candida auris is a multidrug resistant, emerging agent of fungemia in humans. Its actual global distribution remains obscure as the current commercial methods clinical diagnosis misidentify it C. haemulonii. Here we report first draft genome to explore genomic basis virulence and unique differences that could be employed for differential diagnosis. More than 99.5 % reads did not align whole (or draft) sequences albicans, lusitaniae, glabrata Saccharomyces cerevisiae; thereby indicating its...
Hsp90 is important for normal growth and development in eukaryotes. Together with Hsp70 other accessory proteins, not only helps newly synthesized proteins to fold but also regulates activities of transcription factors protein kinases. Although the gene coding heat shock 90 fromPlasmodium falciparum (PfHsp90) has been characterized previously, there very little known regarding its function parasite. We have analyzed PfHsp90 complexes addressed role parasite life cycle using Geldanamycin...
Calnexin is a membrane-bound lectin and molecular chaperone that binds newly synthesized glycoproteins in the endoplasmic reticulum (ER). To analyze oligomeric properties of calnexin calnexin-substrate complexes, sucrose velocity gradient centrifugation chemical cross-linking were used. After CHAPS solubilization Chinese Hamster Ovary cells, unoccupied behaved as monomer sedimenting at 3.5 S20,W. For complexes S-values ranged between 3.5–8 S20,W, size increasing with weight substrate....
Using a pharmacological inhibitor of Hsp90 in cultured malarial parasite, we have previously implicated Plasmodium falciparum (PfHsp90) as drug target against malaria. In this study, biochemically characterized PfHsp90 terms its ATPase activity and interaction with geldanamycin (GA) evaluated potential preclinical mouse model addition, explored the inhibitors drugs for treatment Trypanosoma infection animals. Our studies full-length showed it to highest all known Hsp90s; was 6 times higher...
Abstract New strategies are needed to counter the escalating threat posed by drug-resistant fungi. The molecular chaperone Hsp90 affords a promising target because it supports survival, virulence and drug-resistance across diverse pathogens. Inhibitors of human under development as anticancer therapeutics, however, exert host toxicities that preclude their use antifungals. Seeking route species-selectivity, we investigate nucleotide-binding domain (NBD) from most common fungal pathogen,...
Molecular chaperones participate in the maintenance of cellular protein homeostasis, cell growth and differentiation, signal transduction, development. Although a vast body information is available regarding individual chaperones, few studies have attempted systems level analysis chaperone function. In this paper, we constructed interaction network for malarial parasite, Plasmodium falciparum. P. falciparum responsible several million deaths every year, understanding biology parasite top...
The malaria parasite experiences a significant amount of redox stress during its growth in human erythrocytes and heavily relies on secretory functions for pathogenesis. Most certainly, the is equipped with machinery to tackle perturbations pathway, like unfolded protein response pathway higher eukaryotes. Our bioinformatics analysis revealed complete absence genes involved canonical Plasmodium falciparum. Accordingly, was unable up-regulate endoplasmic reticulum (ER) chaperones or...
Abstract Lumpy skin disease virus (LSDV) belongs to the genus Capripoxvirus and family Poxviridae . LSDV was endemic in most of Africa, Middle East Turkey, but since 2015, several outbreaks have been reported other countries. In this study, we used whole genome sequencing approach investigate origin outbreak understand genomic landscape virus. Our study showed that strain 2022 exhibited many genetic variations compared Reference Neethling sequence previous field strains. A total 1819 were...
The ability of malarial parasite to deploy proteins at the surface infected erythrocytes is well known. After their synthesis within parasite, cargo are exported from and carried across erythrocyte cytoplasm be delivered surface. Our knowledge about mechanisms involved in this complex trafficking path limited. We have addressed involvement chaperones traffic cytoplasm. analyses available indicated that none reported origin present host (Hsp70, Hsp90, Hop60), on other hand, were readily...
The human malarial parasite Plasmodium falciparum (Pf) is exposed to wide temperature fluctuations during its life cycle, ranging from 25 °C in the mosquito vector and 37 humans 41 febrile episodes patient. repeated occurrence of fever at regular intervals a characteristic malaria. We have examined influence exposure elevated temperatures encountered on intraerythrocytic development parasite. Using flow cytometry, we show that mimicking promotes erythrocytes. Heat shock-mediated...
Dry eye syndrome (DES) is a complex, multifactorial, immune-associated disorder of the tear and ocular surface. DES with high prevalence world over needs identification potential biomarkers so as to understand not only disease mechanism but also identify drug targets. In this study we looked for differentially expressed proteins in samples arrive at characteristic biomarkers. As part prospective case-control study, specimen were collected using Schirmer strips from 129 dry cases 73 age...
ABSTRACT Imidazopyridazine compounds are potent, ATP-competitive inhibitors of calcium-dependent protein kinase 1 (CDPK1) and Plasmodium falciparum parasite growth in vitro . Here, we show that these can be divided into two classes depending on the nature aromatic linker between core R2 substituent group. Class have a pyrimidine inhibit at late schizogony, whereas class 2 nonpyrimidine trophozoite stage, indicating different modes action for classes. The also inhibited cyclic GMP...
Recent reports highlight the severity and morbidity of disease caused by long neglected malaria parasite Plasmodium vivax. Due to inherent difficulties in laboratory-propagation P. vivax, biology this has not been adequately explored. While proteome falciparum, causative agent cerebral malaria, extensively explored from several sources, there is limited information on We have, for first time, examined vivax isolated directly patients without adaptation laboratory conditions. have identified...
Thermotolerance is an essential attribute for pathogenesis of Cryptococcus as exemplified by the fact that only two species in genus, which can grow at 37°C, are human pathogens. Species have other virulence factors including capsule formation and melanisation, but lack ability to propagate 37°C not pathogenic. In another related fungal pathogen, Candida albicans, heat shock protein 90 has been implicated be a central player commanding pathogenicity governing yeast hyphal transition drug...