A5020363760- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- SARS-CoV-2 and COVID-19 Research
- Bacterial Identification and Susceptibility Testing
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Enterobacteriaceae and Cronobacter Research
- Genomics and Phylogenetic Studies
- Urinary Tract Infections Management
- Antimicrobial Peptides and Activities
- Vibrio bacteria research studies
- Animal Virus Infections Studies
- Long-Term Effects of COVID-19
- Hepatitis B Virus Studies
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Antifungal resistance and susceptibility
- Patient Satisfaction in Healthcare
- Fungal Infections and Studies
- Inhalation and Respiratory Drug Delivery
King Abdullah International Medical Research Center
2014-2025
King Saud bin Abdulaziz University for Health Sciences
2014-2025
National Guard Health Affairs
2020-2025
Ministry of Health
2024
King Abdulaziz Medical City
2022-2024
Museum of Heilongjiang Province
2021
Public Health England
2018-2019
University of Plymouth
2019
University of Manchester
2014-2015
ObjectivesWe investigated the molecular epidemiology of uropathogenic Escherichia coli (UPEC) from a tertiary care hospital in Riyadh, Saudi Arabia, revealing, for first time, population structure UPEC region.
Galleria mellonella larvae are an alternative in vivo model for investigating bacterial pathogenicity. Here, we examined the pathogenicity of 71 isolates from five leading uropathogenic E. coli (UPEC) lineages using G. larvae. Larvae were challenged with a range inoculum doses to determine 50% lethal dose (LD50) and analysis survival outcome Kaplan-Meier plots. Virulence was correlated carriage panel 29 virulence factors (VF). inoculated ST69 ST127 (104 colony-forming units/larvae) showed...
Methicillin-resistant
Carbapenem-resistant P. aeruginosa has become a major clinical problem due to limited treatment options. However, studies assessing the trends in molecular epidemiology and mechanisms of antibiotic resistance this pathogen are lacking Saudi Arabia. Here, we reported genome characterization global context carbapenem non-susceptible isolates from nationally representative survey. The profiles (n = 635) collected over 14 months between March 2018 April 2019 different geographical regions Arabia...
This study investigated genotypic and phenotypic antimicrobial resistance profiles, phylogenic relatedness, plasmid virulence composition of 39 Salmonella enterica strains isolated from chicken meat samples using whole genome sequencing (WGS) technology. Four distinct serotypes were identified; Minnesota (16/39, 41%), Infantis (13/39, 33.3%), Enteritidis (9/39, 23.1%), one isolate was detected for Kentucky (1/39, 2.6%), with sequence types (STs) as followed: ST548, ST32, ST11, ST198,...
Klebsiella pneumoniae is a major bacterial and opportunistic human pathogen, increasingly recognized as healthcare burden globally. The convergence of resistance virulence in K. strains has led to the formation hypervirulent multidrug-resistant with dual risk, limiting treatment options. clones are known emerge locally spread Therefore, an understanding dynamics evolution emerging hospitals warranted prevent future outbreaks. In this study, we conducted in-depth genomic analysis on...
Abstract Objective: To describe local experience in managing an outbreak of Candida auris a tertiary-care setting. Methods: In response to emerging , investigation was conducted at our hospital between March 2018 and June 2019. Once patient confirmed have screening exposed patients healthcare workers (HCWs) conducted. Postexposure included those who had direct contact with or shared the same unit ward laboratory-confirmed case. increasing number cases, new infection control measures were...
Pandrug-resistant (PDR) K. pneumoniae refractory to conventional treatment has been reported worldwide, causing a huge burden on the healthcare system, patient safety and economy. is prominent opportunistic pathogen hospital-acquired community-acquired infections, but rarely associated with infective endocarditis. Currently, there are sparse data guiding optimal regimen when commonly used antibiotics fail, notably for of endocarditis infections. Here we report our experience in treating...
Objectives Staphylococcus aureus is a leading cause of hospital acquired infections worldwide. Over recent decades, methicillin resistant (MRSA), which to multiple antimicrobials, has emerged as significant pathogenic strain in both and community settings. The rapid emergence dissemination MRSA clones are driven by dynamic evolving population, spreading swiftly across regions on epidemiological time scales. Despite the vast geographical expanse diverse demographics Kingdom Saudi Arabia...
Methicillin-resistant Staphylococcus aureus (MRSA) surveillance in regions with mass gatherings presents unique challenges for public health systems. Saudi Arabia, hosting millions of pilgrims annually, provides a distinctive setting studying how human mobility shapes bacterial populations, yet comprehensive genomic data from this region remains limited. Here, we present an integrated analysis S. isolates collected across seven Arabian regions, combining whole-genome sequencing extensive...
Abstract Background Studying temporal changes in resistant pathogens causing healthcare-associated infections (HAIs) is crucial improving local antimicrobial and infection control practices. The objective was to describe ten-year trends of resistance HAIs a tertiary care setting Saudi Arabia compare such with those US National Health Surveillance Network (NHSN). Methods Pooled analysis surveillance data that were prospectively collected between 2007 2016 four hospitals Ministry Guard...
The antibacterial activity and biofilm reduction capability of liposome formulations encapsulating tobramycin (TL), Tobramycin-N-acetylcysteine (TNL) were tested against tobramycin-resistant strains E. coli, K. pneumoniae A. baumannii in the presence several resistant genes. All assessed bacterial clinical isolate strains, which fully characterized by whole-genome sequencing (WGS). isolates acquired one or more AMEs genes, efflux pump OMP formation TL formulation inhibited growth EC_089...
Background Antibiotics significantly increased life expectancy and decreased mortality rates due to infections. However, this trend is starting fade with the rise of multidrug-resistant organisms (MDR); these strains are becoming a global burden on healthcare economy. The dramatic increase spread carbapenem-resistant gram-negative bacteria (CRGNB) has become serious public health concern. In retrospective cross-sectional study, we aimed estimate bacteremia in five tertiary care hospitals...
Background Fluoroquinolones are some of the most used antimicrobial agents for treatment Pseudomonas aeruginosa . This study aimed at exploring differential activity ciprofloxacin and levofloxacin on selection resistance among P. isolates our medical center. Methods 233 clinical were included in this study. Antimicrobial susceptibility testing (AST) was done using disk diffusion broth microdilution assays. Random Amplification Polymorphic DNA (RAPD) to determine genetic relatedness between...
BACKGROUND: Carbapenems are the antibiotics of last-resort for treatment bacterial infections caused by multidrug-resistant organisms. The emergence resistance is a critical and worrisome problem clinicians patients. Carbapenem-resistant Enterobacterales (CRE) spreading globally, associated with an increased frequency reported outbreaks in many regions, becoming endemic others. OBJECTIVES: Determine molecular epidemiology CRE isolates from various regions Saudi Arabia to identify genes...
E. coli is an Enterobacteriaceae that could develop resistance to various antibiotics and become a multi-drug resistant (MDR) bacterium. Options for treating MDR are limited the pipeline somewhat dry when it comes bacteria, so we aimed explore more options help in coli. The purpose of this study examine synergistic effect liposomal formulations co-encapsulated azithromycin N-acetylcysteine against Liposomal (LA) azithromycin/N-acetylcysteine (LAN) were compared free azithromycin. A broth...
Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have been isolated from human patients in many countries across the globe but rarely Saudi Arabia. Here we provide genomic characterization of first KPC-producing K. urine a patient Riyadh, Arabia, who had recent travel history to Egypt involving medical procedure. Presence KPC-encoding gene initially detected with Xpert Carba-R assay was confirmed by traditional PCR. Susceptibility testing using VITEK 2 system, E-test...
The misuse of antibiotics is leading to the emergence multidrug-resistant (MDR) bacteria, and in absence available treatments, this has become a major global threat. In middle recent severe acute respiratory coronavirus 2 (SARS-CoV-2) pandemic, which challenged whole world, MDR bacteria increasing due prophylactic administration intensive care unit patients prevent secondary bacterial infections. This just an example underscoring need seek alternative treatments against bacteria. To end,...
Non-typhoidal Salmonella (NTS) strains are Gram negative bacterial pathogens that associated with foodborne illness worldwide. During the process of infection, uses two molecular injectisomes known as Type 3 Secretion Systems (T3SS) to secrete virulence factors encoded by Pathogenicity Island-1 (SPI-1) and SPI-2 into host cells. These secretion systems play a major role in virulence, shown various animal models, but little is about their human infections. In Saudi Arabia, NTS frequently...
Middle East respiratory syndrome coronavirus (MERS-CoV) has continued to cause sporadic outbreaks of severe tract infection over the last 8 years. Complete genome sequencing using next-generation was performed for MERS-CoV isolates from cases that occurred in Riyadh between 2015 and 2019. Phylogenetic analysis molecular mutational were carried out investigate disease severity. A total eight subjected complete sequencing. resulted assembly 7/8 sequences within lineage 3 one sequence 4 showing...
Uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infections (UTIs) in hospitalised and non-hospitalised patients. Genomic analysis was used to gain further insight into molecular characteristics UPEC isolates from Saudi Arabia. A total 165 were collected patients with UTIs between May 2019 September 2020 two tertiary hospitals Riyadh, Identification antimicrobial susceptibility testing (AST) performed using VITEK system. Extended-spectrum β-lactamase...
The recent declaration by the United Nations to establish an interagency coordination group (IACG) on antimicrobial resistance (AMR) emphasises global nature of AMR threat. Rapid dissemination and spread is exacerbated movements humans, animals, foods materials. International monitoring surveillance indicates policy makers, regulators auditors magnitude problem also informs appropriate mindful interventions that will impact public health mitigate AMR. Identifying drivers requires a...
We report the complete genome sequence of a colistin-resistant strain uropathogenic Escherichia coli, isolated in January 2013 at King Abdulaziz Medical City (KAMC), Riyadh, Saudi Arabia. The isolate (named SA186) was type 131 (ST131) and belonged to serotype O25b-H4 clade B (fimH22).
Klebsiella pneumoniae is a Gram-negative bacterium associated with wide range of community- and hospital-acquired infections. The emergence clonal hypervirulent strains resistant to last-resort antimicrobial agents has become global concern. Kingdom Saudi Arabia (KSA), its diverse population high tourism traffic, serves as platform where the spread multidrug-resistant (MDR) are facilitated. However, knowledge epidemiology diversity MDR K. in KSA scarce. We conducted comprehensive genomic...