A5008235309- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Vibrio bacteria research studies
- Tuberculosis Research and Epidemiology
- Antimicrobial agents and applications
- Diagnosis and treatment of tuberculosis
- Gut microbiota and health
- Essential Oils and Antimicrobial Activity
- Escherichia coli research studies
- Antimicrobial Peptides and Activities
- Animal Diversity and Health Studies
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Helicobacter pylori-related gastroenterology studies
- Electrospun Nanofibers in Biomedical Applications
- Urinary Tract Infections Management
- Wastewater Treatment and Nitrogen Removal
- Wound Healing and Treatments
- Infection Control in Healthcare
- Clostridium difficile and Clostridium perfringens research
- Microbial Fuel Cells and Bioremediation
- Biosensors and Analytical Detection
- Diet and metabolism studies
- Gastric Cancer Management and Outcomes
King Abdulaziz City for Science and Technology
2014-2024
PRG S&Tech (South Korea)
2024
Bridge University
2024
King Saud University
2024
Texas Children's Hospital
2003-2007
Northeastern University
2003-2007
The infection and prevalence of extended-spectrum β-lactamases (ESBLs) is a worldwide problem, the presence ESBLs varies between countries. In this study, we investigated occurrence plasmid-mediated ESBL/AmpC/carbapenemase/aminoglycoside resistance gene expression in Escherichia coli using phenotypic genotypic techniques.A total 58 E. isolates were collected from hospitals city Makkah screened for production genes. All samples subjected to analyses. antibiotic susceptibility was determined...
Abstract: We investigated the efficacy of liposomal gentamicin formulations different surface charges against Pseudomonas aeruginosa and Klebsiella oxytoca . The were prepared by dehydration–rehydration method, their sizes zeta potential measured. Gentamicin encapsulation efficiency inside was determined microbiologic assay, stability in biologic fluid evaluated for a period 48 h. minimum inhibitory concentration bactericidal determined, vitro time kill studies free form performed....
Acinetobacter baumannii is a common opportunistic pathogen that causes major nosocomial infections in hospitals. In this study, we hypothesized high prevalence of A. baumanni ESBL (extended-spectrum beta-lactamase) among all collected isolates. isolates (n = 107) from ICU (Intensive care unit) local hospitals Makkah were phenotypically and genotypically characterized. The identity antibiotic susceptibility strains determined using the Vitek-2 system. identified producers further analyzed by...
Hand hygiene is an essential factor to prevent or minimize the spread of infections. The ability prepare alcohol-free hand sanitizer (AFHS) with antimicrobial properties crucial, especially during pandemics, when there are high demands and a low supply chain for ethanol isopropanol. objective this study was AFHS gels based on natural materials that contain oils (EOs) would be effective against broad spectrum pathogens. results showed organoleptic characteristics all prepared were considered...
Escherichia coli is a common cause of biofilm-associated urinary tract infections (UTIs). Biofilm formation in E. responsible for various indwelling medical device-associated infections, including catheter-associated (CAUTIs). This study aimed to reduce biofilm ATCC 25922 by knocking out genes involved quorum sensing (QS) (luxS) and adhesion (fimH bolA) using the CRISPR/Cas9-HDR approach.Single-guide RNAs (sgRNAs) were designed target luxS, fimH bolA genes. Donor DNA homologous recombination...
Introduction: Diabetes mellitus is frequently associated with foot ulcers, which pose significant health risks and complications. Impaired wound healing in diabetic patients attributed to multiple factors, including hyperglycemia, neuropathy, chronic inflammation, oxidative damage, decreased vascularization. Rationale: To address these challenges, this project aims develop bioactive, fast-dissolving nanofiber dressings composed of polyvinylpyrrolidone loaded a combination an antibiotic...
Mycobacterium avium subspecies paratuberculosis (M. ap) is the causative agent of or Johne's disease (JD) in herbivores with potential involvement cases Crohn's humans. JD spread worldwide and economically important for both beef dairy industries. Generally, pathogenic ovine strains ap-S) are mainly found sheep while bovine ap-C) infect other ruminants (e.g. cattle, goat, deer), as well sheep. In an effort to characterize this emerging infection dromedary/Arabian camels, we successfully...
Hand hygiene is one of the effective measures for reducing transmission infections. Alcohol-based hand sanitizers containing ethanol or isopropanol are considered efficient alternatives to handwashing with water and soap. Despite being against a broad-spectrum microbes, fining an alternative alcohol-based became necessity owning limitations associated their use, such as skin dryness, irritant contact dermatitis, intoxication upon accidental ingestion. Furthermore, in certain circumstances...
The inadequate eradication of pulmonary infections and chronic inflammation are significant complications in cystic fibrosis (CF) patients, who usually suffer from persistent frequent lung caused by several pathogens, particularly Pseudomonas aeruginosa (P. aeruginosa). ability pathogenic microbes to protect themselves biofilms leads the development an innate immune response antibiotic resistance. In present work, a reference bacterial strain P. (PA01) multidrug-resistant isolate (MDR 7067)...
Acinetobacter baumannii is known for nosocomial outbreaks worldwide. In this study, we aimed to investigate the antibiotic susceptibility patterns and clonal relationship of A. isolates from intensive care unit (ICU) an Egyptian hospital. present 50 clinical multidrug resistant (MDR)-A. were obtained patients admitted into ICU June December 2015. All analyzed antimicrobial susceptibilities. Multiplex PCR was performed detect genes encoding oxacillinase (bla OXA-51-like, bla OXA-23-like,...
Skin infection compromises the body's natural defenses. Several antibiotics are no longer effective owing to evolution of antimicrobial-resistant (AMR) bacteria, hence, constant development novel antibacterial agents. Naturally occurring agents may be potential candidates for AMR bacterial treatments; however, caution should taken when administering such due high incidence toxicity. A fibrous material system from a biocompatible polymer that could used as skin patch infections treatment...
Methicillin-resistant Staphylococcus aureus (MRSA) surveillance in regions with mass gatherings presents unique challenges for public health systems. Saudi Arabia, hosting millions of pilgrims annually, provides a distinctive setting studying how human mobility shapes bacterial populations, yet comprehensive genomic data from this region remains limited. Here, we present an integrated analysis S. isolates collected across seven Arabian regions, combining whole-genome sequencing extensive...
Despite its economical, cultural, and biological importance, there has not been a large scale sequencing project to date for Camelus dromedarius. With the goal of complete DNA organism, we first established sequenced camel EST libraries, generating 70,272 reads. Following trimming, chimera check, repeat masking, cluster assembly, obtained 23,602 putative gene sequences, out which over 4,500 potentially novel or fast evolving sequences do carry any homology other available genomes. Functional...
Background and Aim . Because genetic geographic variations in intestinal microbiota are known to exist, the focus of this study was establish an estimation colorectal cancer (CRC) patients Saudi Arabia by means metagenomic studies. Methods From July 2010 November 2012, attending our hospital were enrolled for All underwent clinical, endoscopic, histological assessment. Mucosal samples collected from each patient jet-flushing colonic mucosa with distilled water at unified segments colon,...
The intestinal microbiota plays an essential role in the pathogenesis of ulcerative colitis (UC)and Crohn disease (CD).Metagenomic studies were used to study diagnosed cases UC and CD at King Fahad Medical City, Riyadh, Saudi Arabia. Each segment colon was flushed with distilled water during colonoscopy, material aspirated, immediately frozen for study. patients attending screening colonoscopies taken as age-matched healthy controls. followed clinically any signs exacerbation relapse,...
Artificial intelligence (AI) is a new technology that has been employed to screen and discover drugs. Using AI, an anti-diabetic treatment (Halicin) was nominated proven have unique antibacterial activity against several harmful bacterial strains, including multidrug-resistant bacteria. This study aims explore the effect of halicin microbial susceptibility using zone inhibition minimum concentration (MIC) values while assessing stability stored over period time with cost-effective...
Since the COVID-19 pandemic outbreak in world, many countries have searched for quick diagnostic tools to detect virus. There are ways design assays; however, each may its limitations. A quick, sensitive, specific, and simple approach is essential highly rapidly transmitted infections, such as SARS-CoV-2. This study aimed develop a rapid cost-effective tool using one-step Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) approach. The results were observed naked eye...
Pressure ulcer or bedsore is a form of skin infection that commonly occurs with patients admitted to the hospital for an extended period time, which might lead severe complications in absence medical attention, resulting either by drug-sensitive drug-resistant bacteria. Halicin, newly discovered drug effective against several bacterial strains, including multidrug-resistant bacteria, was investigated reduce burden. This study aims formulate halicin into electrospun fibers be applied bedsores...
The incidence of extrapulmonary tuberculosis (EPTB) in low- and middle-income countries, as well as, high-income countries has increased over the last two decades. acid-fast bacillus (AFB) smear test is easy to perform cost-effective with a quick turnaround time but low sensitivity. Culture remains gold standard for detecting TB; however, it sensitivity slow bacterial growth patterns, may take up 6 8 weeks grow. Therefore, rapid detection tool crucial early initiation treatment ensuring an...
In the Kingdom of Saudi Arabia (KSA), Old World cutaneous leishmaniasis (CL) is mainly caused by Leishmania major and tropica parasites. Diagnosis CL predominately made clinicians, who at times fail to detect disease are unable identify parasite species. Here, we report development a chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) measure levels anti-α-galactosyl antibodies in human sera. Using this assay, have found that individuals infected with either spp. had significantly...
In the Kingdom of Saudi Arabia (KSA), Leishmania major and L. tropica are main causative agents Old World cutaneous leishmaniasis (CL). The national CL treatment regimen consists topical 1% clotrimazole/2% fusidic acid cream followed by 1–2 courses intralesional sodium stibogluconate (SSG); however, efficacy is highly variable reasons for this not well understood. study, we present a complete epidemiological map determined standard regime in several endemic regions KSA. Overall, three...
Abstract Background The focus of this study was to explore potential differences in colonic mucosal microbiota irritable bowel syndrome (IBS) patients compared a control group utilizing metagenomic study. Methods Mucosal samples were collected from each IBS patient jet‐flushing mucosa unified segments the colon with distilled water, followed by aspiration, during colonoscopy. All purified dsDNA extracted and quantified before sequencing using an Illumina platform. An equal number healthy...