A5030781607- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- SARS-CoV-2 detection and testing
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Vectors
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- COVID-19 epidemiological studies
- Mosquito-borne diseases and control
- Liver Disease and Transplantation
- Malaria Research and Control
- Biosensors and Analytical Detection
- Travel-related health issues
- Animal Diversity and Health Studies
- Respiratory viral infections research
- Animal health and immunology
- Influenza Virus Research Studies
- Long-Term Effects of COVID-19
- Pneumonia and Respiratory Infections
- Pharmacological Effects of Natural Compounds
King Abdulaziz University
2016-2025
Menoufia University
2005-2023
King Fahd Medical City
2022
University of Jeddah
2021
Umm al-Qura University
2021
King Fahad Hospital Jeddah
2017
National Water Research Center
2007-2014
Minia University Hospital
2007
Liver Institute Northwest
2002-2006
Cairo University
2005
We describe the isolation and sequencing of Middle East respiratory syndrome coronavirus (MERS-CoV) obtained from a dromedary camel patient who died laboratory-confirmed MERS-CoV infection after close contact with camels that had rhinorrhea. Nasal swabs collected one his nine were positive for RNA. In addition, was isolated camel. The full genome sequences two isolates identical. Serologic data indicated circulating in but not before human occurred. These suggest this fatal case transmitted...
Dengue viruses (DENVs) are mosquito-borne which can cause disease ranging from mild fever to severe dengue infection. These endemic in several tropical and subtropical regions. Multiple outbreaks of DENV serotypes 1, 2 3 (DENV-1, DENV-2 DENV-3) have been reported the western region Saudi Arabia since 1994. Strains at least two genotypes DENV-1 (Asia America/Africa genotypes) circulating until 2006. However, all previous studies were based on partial sequencing data envelope (E) gene without...
Egypt has one of the world's highest prevalences hepatitis C virus (HCV) infection, with a majority genotype 4 infections. To explore genetic diversity HCV in Egypt, sera from 131 Egyptians [56 community studies, 37 chronic patients, 28 hepatocellular carcinoma (HCC) patients and 10 non-Hodgkin's lymphoma] were genotyped by restriction fragment-length polymorphism phylogenetic analyses sequences mid-core non-structural 5B regions. The different genotyping methods showed good agreement....
Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel betacoronavirus that has been circulating in the Arabian Peninsula since 2012 and causing severe infections humans. While bats were suggested to be involved human MERS-CoV infections, direct link between uncertain. On other hand, serological virological data suggest dromedary camels as potential animal reservoirs of MERS-CoV. Recently, we isolated from camel its infected owner provided evidence for transmission patient. Here,...
Clinical detection of Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) in patients is achieved using genetic diagnostic methods, such as real-time RT-PCR assay. Previously, we developed a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the MERS-CoV [Virol J. 2014. 11:139]. Generally, RT-LAMP monitored by turbidity induced precipitation magnesium pyrophosphate with newly synthesized DNA. However, this mechanism cannot completely exclude...
Mycotoxins such as the aflatoxins and deoxynivalenol (DON) are frequent contaminants of food. Aflatoxin B1 (AFB1) DON affect immune system restrict growth; additionally AFB1 is carcinogenic. To date there limited descriptive biomarker data concerning maternal exposures during pregnancy, none on co-exposures to these mycotoxins. This survey was a cross-sectional assessment providing concentrations serum aflatoxin–albumin (AF-alb), urinary aflatoxin M1 (AFM1), for 98 pregnant women from Egypt,...
SummaryBackgroundThe Middle East respiratory syndrome coronavirus (MERS-CoV) is a lethal zoonotic pathogen endemic to the Arabian Peninsula. Dromedary camels are likely source of infection and virus probably originated in Africa. We studied genetic diversity, geographical structure, prevalence, age-associated prevalence among at largest entry port from Africa into Peninsula.MethodsIn this prospective genomic study, we took nasal samples imported Sudan Djibouti Port Jeddah Jeddah, Saudi...
Genetic factors such as the HLA type of patients may play a role in regard to disease severity and clinical outcome with COVID-19. Taking data deposited GISAID database, we made predictions using IEDB analysis resource (TepiTool) gauge how variants SARS-CoV-2 genome change peptide binding most frequent MHC-class I -II alleles Africa, Asia Europe. We caracterized single mutation wildtype sequence could influence MHC class II, but not alleles. Assuming ORF8 (L84S) is biologically significant,...
Abstract Although persistent transmission of hepatitis C virus (HCV) from infected mothers to their infants is reported in 4–8%, transient HCV perinatal infection also occurs. This prospective cohort study determined infection‐ and early late clearance‐rates 1,863 mother‐infant pairs rural Egyptian villages. found 15.7% 10.9% pregnant women had antibodies (anti‐HCV) HCV‐RNA, respectively. Among 329 born these mothers, 33 (10.0%) tested positive for both anti‐HCV HCV‐RNA 2 months following...
BACKGROUND Middle East respiratory syndrome‐coronavirus (MERS‐CoV) is a novel zoonotic pathogen. Although the potential for MERS‐CoV transmission through blood transfusion not clear, was recognized as pathogen of concern safety supply especially after its detection in whole blood, serum, and plasma infected individuals. Here we investigated efficacy amotosalen ultraviolet A light (UVA) to inactivate fresh‐frozen (FFP). STUDY DESIGN AND METHODS Pooled FFP units were spiked with recent...
The World Health Organization priority zoonotic pathogen Middle East respiratory syndrome (MERS) coronavirus (CoV) has a high case fatality rate in humans and circulates camels worldwide.We performed global analysis of human camel MERS-CoV infections, epidemiology, genomic sequences, clades, lineages, geographical origins for the period January 1, 2012 to August 3, 2022. Surface gene sequences (4061 bp) were extracted from GenBank, phylogenetic maximum likelihood tree was constructed.As...
<title>Abstract</title> One of the biggest obstacles in applying metagenomics clinical field is increase human genome reads resulting sequences. In this study, three depletion methods were compared that utilize methylation cytosine DNA as a mechanism action. Two commercial kits NEBNext Microbiome Enrichment Kit and MethylMiner™ Methylated bind methylated cytosines to specific antibodies, along with MspJI restriction enzyme digests cytosines. The applied HeLa cell suspension (as source for...
Supplemental assays, such as recombinant immunoblot assays (RIBA), are used to confirm detection of antibodies hepatitis C virus (HCV). However, due their expense, they not widely in developing countries. The purpose our study was compare the results second- and third-generation (G2 G3, respectively) enzyme immunoassays (EIAs) resolve discordant by using a supplemental assay assess reliability G2 G3 EIAs anti-HCV antibody-positive results. We performed both for on 1,134 serum samples...
Background. Acute viral hepatitis is less frequent in Egypt than serum antibody levels suggest. Because acute has a wide clinical spectrum, we tested the hypothesis that many cases are undetected because of mild illness caused by initial, early-childhood exposure to viruses. Methods. During active case detection among 20,000 inhabitants rural villages Egypt, screened 1715 symptomatic patients for alanine aminotransferase (ALT) levels. Viral markers were 47 subjects who had ALT least twice...
A prospective cohort study of the incidence and risk factors for hepatitis C virus (HCV) infection was performed in 2171 pregnant women three rural Egyptian villages who were HCV antibody (anti-HCV) RNA (HCV-RNA) negative at baseline. During an average 2.2 years follow up, 25 incident cases observed, giving estimated 5.2/1000 person-years (PY). The rate correlated with community anti-HCV prevalence women, while perinatal 11.2/1000 PY almost five times that non-perinatal (2.3/1000 PY). data...
Single-nucleotide polymorphisms (SNPs) around IL28B are associated with spontaneous hepatitis C virus (HCV) clearance of genotypes 1 and 3 in white African-American populations. This study investigated whether the SNP (rs12979860) is HCV, principally genotype 4, 162 Egyptians (80 clearance). The protective allele was more common those (76.3% vs 57.9%; P = .0006). Individuals were 3.4 (95% confidence interval, 1.8-6.5) times likely to have C/C genotype. Thus, plays a role HCV 4 North Africa.
SUMMARY Objective This study aimed to assess the efficacy of INTERCEPT™ Blood System [amotosalen/ultraviolet A (UVA) light] reduce risk Middle East respiratory syndrome‐Coronavirus (MERS‐CoV) transmission by human platelet concentrates. Background Since 2012, more than 2425 MERS‐CoV cases have been reported in 27 countries. The infection causes acute disease, which was responsible for 838 deaths these countries, mainly Saudi Arabia. Viral genomic RNA detected whole blood, serum and plasma...