A5052520741- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
- Computational Drug Discovery Methods
- RNA Research and Splicing
- Insect Pest Control Strategies
- COVID-19 epidemiological studies
- Cervical Cancer and HPV Research
- Virus-based gene therapy research
- Hepatitis B Virus Studies
- Pharmacological Effects of Natural Compounds
- Research on Leishmaniasis Studies
- Travel-related health issues
- Biosensors and Analytical Detection
- Cancer-related Molecular Pathways
- Phytochemistry and Biological Activities
- Genital Health and Disease
- Viral Infections and Outbreaks Research
- Ferroptosis and cancer prognosis
- Poxvirus research and outbreaks
- Insect Resistance and Genetics
- Hepatitis Viruses Studies and Epidemiology
- Bacillus and Francisella bacterial research
King Abdulaziz University
2020-2025
University of Glasgow
2016-2024
MRC University of Glasgow Centre for Virus Research
2016-2020
Institute of Infection and Immunity
2016
Due to the adverse effects of obesity on host immunity, this study investigated effectiveness COVID-19 vaccines (BNT162b2, ChAdOx-nCov-2019, and mRNA-1273) in inducing anti-SARS-CoV-2 Spike (S) neutralizing antibodies among individuals with various classes (class I, II, III, super obesity). Sera from vaccinated obese (n = 73) normal BMI controls 46) were subjected S-based enzyme-linked immunosorbent assay (ELISA) serum-neutralization test (SNT) determine prevalence titer antibodies....
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a more severe strain of coronavirus (CoV) that was first emerged in China 2019. Available antiviral drugs could be repurposed and natural compounds with activity safer cheaper source medicine for SARS-CoV-2. 78 database identified from literature virtual screening technique applied to identify potential 3-chymotrypsin-like protease (3CLpro) inhibitors. Molecular docking studies were conducted analyze the main inhibitors...
Human papillomavirus (HPV), a common sexually transmitted infection, includes over 200 types, some linked to genital warts and various cancers, including cervical, anal, penile, oropharyngeal cancers. In Saudi Arabia, an estimated 10.7 million women aged 15 years older are at risk of HPV-related cervical cancer. This study assessed HPV prevalence, genotype distribution, awareness, health behaviors among females in Jeddah, Arabia. Pap smear samples were collected from 106 attending gynecology...
The ongoing coronavirus disease 19 (COVID-19) pandemic, caused by the novel severe acute respiratory syndrome 2 (SARS-CoV-2), poses a threat to human health. Despite this, many affected countries are now in process of gradual lifting COVID-19 restrictions that were initially implemented response pandemic. success so-called “exit strategy” requires continued surveillance virus circulation community and evaluation prevalence protective immunity among population. Serology tests valuable tools...
Abstract Japanese encephalitis (JE) virus is a flavivirus causing neurological damage. RNA-dependent-RNA-polymerase (RdRp) responsible for genome replication making it excellent anti-viral target. In this study, the crystal structure of JE RdRp (jRdRp) and bioflavonoids reported in Azadirachta indica were retrieved from specific databases. Structure-based virtual screening was employed using MTiOpenScreen server top four compounds selected with most negative docking scores. Conformations...
The infectious life cycle of human papillomaviruses (HPVs) is tightly linked to keratinocyte differentiation. Evidence suggests a sophisticated interplay between host gene regulation and virus replication. Alternative splicing an essential process for viral expression, generally upregulated by serine arginine-rich factors (SRSFs). SRSF activity can be positively or negatively controlled cycles phosphorylation/dephosphorylation. Here we show that HPV16 infection leads accumulation the...
The Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that causes viral leading to neural damage, is major threat in most Asian countries. RNA-dependent RNA polymerase (RdRp) present the genome key component for replication, making it an attractive target antiviral drug development. In this study, natural products from Echinacea angustifolia were retrieved structure-based virtual screening against JEV–RdRp. top six compounds (Echinacoside, Echinacin, Rutin, Cynaroside,...
Zika virus (ZIKV) has been characterized as one of many potential pathogens and placed under future epidemic outbreaks by the WHO. However, a lack therapeutics can result in an uncontrolled pandemic with other human viruses. Therefore, prioritized effective development recommended against ZIKV. In this context, present study adopted strategy to explore lead compounds from Azadirachta indica ZIKV via concurrent inhibition NS2B-NS3 protease (ZIKVpro) NS5 RNA dependent polymerase (ZIKVRdRp)...
Dengue virus (DENV) is the causative agent of DENV infection. To tackle infection, development therapeutic molecules as direct-acting antivirals (DAAs) has been demonstrated a truly effective approach. Among various drug targets, non-structural protein 5 (NS5)—a highly conserved among family Flaviviridae—carries RNA-dependent RNA polymerase (DENVRdRp) domain at C-terminal, and its “N-pocket” allosteric site widely considered for anti-DENV development. Therefore, in this study, we developed...
Data integration with phenotypes such as gene expression, pathways or function, and protein-protein interactions data has proven to be a highly promising technique for improving human complex diseases, particularly cancer patient outcome prediction. Hepatocellular carcinoma is one of the most prevalent cancers, common cause chronic HBV HCV infection, which linked majority cases, play role in multistep carcinogenesis progression. We examined list known hepatocellular biomarkers publicly...
A few months ago, the availability of a reliable and cost-effective testing capacity for COVID-19 was concern many countries. With emergence circulation new SARS-CoV-2 variants, another layer challenge can be added at both molecular serological levels. This is particularly important available tests principally designed to target S gene/protein where multiple mutations have been reported. Herein, NP recombinant protein utilized develop simple human IgG ELISA. The optimized protocol validated...
In response to the coronavirus disease 2019 (COVID-19), Saudi Arabia have imposed timely restrictions minimize infection spread, lower risk for vulnerable groups, and reduce pressure on healthcare services. The effectiveness of these measures has not been assessed comprehensively and, thereby, remains uncertain. Besides monitoring number COVID-19 cases diagnosed by molecular assays, seroprevalence can serve as an indicator incidence rate among general population. This study aimed evaluate...
A therapy for COVID-19 (Coronavirus Disease 19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) remains elusive due to the lack of an effective antiviral therapeutic molecule. The SARS-CoV-2 main protease (M pro ), which plays a vital role in viral life cycle, is one most studied and validated drug targets. In Several prior studies, numerous possible chemical entities were proposed as potential M inhibitors; however, failed at various stages discovery. Repositioning...
The aim of our study was to define the spectrum viral infections in pilgrims with acute respiratory tract illnesses presenting healthcare facilities around holy places Makkah, Saudi Arabia during 2019 Hajj pilgrimage. During five days Hajj, a total 185 were enrolled study. Nasopharyngeal swabs (NPSs) 126/185 patients (68.11%) tested positive for one or more viruses by PCR. Among 126 whose NPS PCR positive: (a) there 93/126 (74%) single virus infection, (b) 33/126 (26%) coinfection than (up...
Iron is a crucial micronutrient for immunity induction in response to infections and vaccinations. This study aimed investigate the effect of iron deficiency on COVID-19-vaccine-induced humoral immunity. We investigated effectiveness COVID-19 vaccines (BNT162b2, mRNA-1273, ChAdOx nCov-2019) iron-deficient individuals (n = 63) provide side-by-side comparison healthy controls 67). The presence anti-SARS-CoV-2 spike (S) anti-nucleocapsid (NP) IgG were assessed using in-house S- NP-based ELISA...
The new coronavirus disease 2019 (COVID-19) is a major health problem worldwide. surveillance of seropositive individuals serves as an indicator to the extent infection spread and provides estimation herd immunity status among population. Reports from different countries investigated this issue healthcare workers (HCWs) who are "at risk" "sources for COVID-19. This study aims investigate seroprevalence COVID-19 HCWs in one referral centers Makkah, Saudi Arabia using three serological...
In the era of SARS-CoV-2 variants and COVID-19 vaccination, duration infectious viral shedding isolation in post vaccine breakthrough infections is challenging depends on disease severity. The current study described a case Delta variant pneumonia requiring hospitalization. patient received two doses BNT162b2 vaccines, he had positive cultures 12 days symptom onset. time between second dose infection was 6 months. While immunosuppression known risk factor for prolonged shedding, age...
ABSTRACT Human papillomaviruses (HPV) infect epithelial causing benign lesions. However, the so-called “high risk” HPVs that anogenital regions and oropharynx can cause precancerous lesions progress to malignant tumours. Understanding HPV life cycle is important discovery of novel antiviral therapies. uses cellular splicing for production full suite viral mRNAs. Members serine/arginine rich (SR) protein family positively regulate splicing. SR activity location regulated by kinases through...
Marburg virus infection poses a significant threat to humans due its high fatality rate. The application of in-silico drug design target the essential protein has been proven be fundamental technique inhibit viral growth. Here, VP40 (a matrix protein) was used as an Marburg, and 2569 natural compounds were screened using molecular docking neural network-based DeepPurpose architecture. top 138 that exhibited binding score −8 kcal/mol in best model predict IC50. composed Morgan CNN-based...
This study investigated the presence of COVID-19 cases among pilgrims, healthcare workers, and non-healthcare workers Hajj 2022. Nasopharyngeal samples were collected from 491 attendees All participants received three doses vaccines. Paired (n = 138; 69 participants) unpaired 422; 422 nasopharyngeal swabs subjected to RT-PCR targeting E gene β-coronaviruses RNA-dependent RNA polymerase SARS-CoV-2. Results linked participants' profiles including role during Hajj, respiratory symptoms or...