A5031824191- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Cervical Cancer and HPV Research
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Genital Health and Disease
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Long-Term Effects of COVID-19
- CRISPR and Genetic Engineering
- Neonatal Respiratory Health Research
- Pregnancy and preeclampsia studies
- Endometrial and Cervical Cancer Treatments
- Liver Disease Diagnosis and Treatment
- Extracellular vesicles in disease
- Reproductive tract infections research
- Hepatitis C virus research
- Preterm Birth and Chorioamnionitis
- Bacteriophages and microbial interactions
- Reproductive System and Pregnancy
- Global Cancer Incidence and Screening
- COVID-19 epidemiological studies
- Analytical Chemistry and Sensors
King Faisal Specialist Hospital & Research Centre
2015-2025
Alfaisal University
2016-2025
University of Idaho
2012
Simple, rapid, specific, and sensitive point-of-care detection methods are needed to contain the spread of SARS-CoV-2. CRISPR/Cas9-based lateral flow assays emerging as a powerful alternative for COVID-19 diagnostics. Here, we developed Bio-SCAN (biotin-coupled specific CRISPR-based assay nucleic acid detection) an accurate pathogen platform that requires no sophisticated equipment or technical expertise. detects SARS-CoV-2 genome in less than 1 h from sample collection result. In first...
The major cause of death in SARS-CoV-2 infected patients is due to de-regulation the innate immune system and development cytokine storm. infects multiple cell types lung, including macrophages, by engagement its spike (S) protein on angiotensin converting enzyme 2 (ACE2) receptor. ACE2 receptor initiates signals macrophages that modulate their activation, production cytokines chemokines. IL-1R-associated kinase (IRAK)-M a central regulator inflammatory responses regulating magnitude TLR...
Type VI CRISPR-Cas systems have been repurposed for various applications such as gene knockdown, viral interference, and diagnostics. However, the identification characterization of thermophilic orthologs will expand unlock potential diverse biotechnological applications. Herein, we identified characterized a thermostable ortholog Cas13a family from organism Thermoclostridium caenicola (TccCas13a). We show that TccCas13a has close phylogenetic relation to HheCas13a bacterium Herbinix...
Rapid, specific, and sensitive detection platforms are prerequisites for early pathogen to efficiently contain control the spread of contagious diseases. Robust portable point-of-care (POC) methods indispensable mass screening SARS-CoV-2. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)-based nucleic acid technologies coupled with isothermal amplification provide a straightforward easy-to-handle platform detecting SARS-CoV-2 at POC,...
Background SARS-CoV-2 infects through the respiratory route and triggers inflammatory response by affecting multiple cell types including type II alveolar epithelial cells. signals via its Spike (S) protein, which have been shown to participate in pathogenesis of COVID19. Aim present study was investigate effect SARS-CoV2 on cells, focusing initiated S protein their impact expression mediators. Results For this purpose A549 cells were exposed SARS CoV2 recombinant mediators measured. The...
Complement protein C1q is the first recognition subcomponent of complement classical pathway that plays a vital role in clearance immune complexes, pathogens, and apoptotic cells. also has homeostatic involving non-immune cells; these functions not necessarily involve activation. Recently, been shown to be expressed locally microenvironment range human malignant tumors, where it can promote cancer cell adhesion, migration, proliferation, without present ascitic fluid formed during ovarian...
Abstract Introduction: The global incidence of coronavirus disease 2019 (COVID-19) has increased significantly due to the Omicron wave. interaction between host factors, including immune status and vaccination history, along with circulating SARS-CoV-2 variant, collectively influences clinical outcomes following exposure COVID-19. Material Methods: This retrospective study examined patients infected COVID-19 during BA.5 wave at King Faisal Specialist Hospital Research Center in Jeddah, Saudi...
Background Coronavirus disease (COVID-19) is reported as a complex disorder affecting multiple systems and coagulopathy that can cause mortality. In this study, we investigated the correlation of SARS-CoV-2 mutations found in blood samples with various changes fibrinolysis system, well severity based on outcome whether or not these patients were admitted into ICU. Materials methods COVID-19 (n = 446) to our institute between 2021 2022 recruited. Blood collected, sequence analysis spike gene...
Objectives As of 1 November 2015, the Saudi Ministry Health had reported 1273 cases Middle East respiratory syndrome (MERS); among these cases, which included 9 outbreaks at several hospitals, 717 (56%) patients recovered, 14 (1%) remain hospitalised and 543 (43%) died. This study aimed to determine epidemiological, demographic clinical characteristics that distinguished MERS contracted during from those sporadically (ie, non-outbreak) between 2012 2015 in Arabia. Design Data confirmed...
The genome of severe acute respiratory coronavirus-2 (SARS-CoV-2), the virus responsible for coronavirus disease 2019 (COVID-19), has undergone a rapid evolution, resulting in emergence multiple SARS-CoV-2 variants with amino acid changes. This study aimed to sequence whole and detect present specimens from Saudi Arabia. Furthermore, we sought analyze characterize changes various proteins identified variants. A total 1161 samples patients diagnosed COVID-19 Arabia, between 1 April 2021 31...
Abstract This study introduces an innovative electrochemical aptasensor designed for the highly sensitive and rapid detection of Legionella pneumophila serogroup 1 ( L. SG1 ), a particularly virulent strain associated with Legionellosis. Employing rigorous selection process utilizing cell-based systematic evolution ligands by exponential enrichment (cell-SELEX), we identified new high-affinity aptamers specifically tailored . The encompassed ten rounds cell-SELEX cycles live , including...
Human papillomavirus (HPV) is a causative agent of cervical and other cancers. Sexually transmitted Infections (STIs) may play crucial role in HPV persistence, leading to serious complications, including cancer. This study investigated the association HPV/STI co-infection samples with dysplasia among women Saudi Arabia. HPV-positive (n = 142) were obtained from previous studies newly collected 209) aged 19-83 years. For detection genotyping, PCR Genoflow assay kits used. STIs detected using...
Syncytiotrophoblast derived Extracellular Vesicles (STBEV) from normal pregnancy (NP) have previously been shown to interact with circulating monocytes and B cells induce pro-inflammatory cytokine release. Early-onset preeclampsia (EOPE) is associated an exacerbated inflammatory response, yet there little data regarding late-onset PE (LOPE) immune function. Here, using a macrophage/monocyte cell line THP-1, we investigated the potential of STBEV, comprising medium/large-STBEV (>200nm)...
SARS-CoV-2 genomic mutations outside the spike protein that may increase transmissibility and disease severity have not been well characterized. This study identified in nucleocapsid their possible association with patient characteristics. We analyzed 695 samples from patients confirmed COVID-19 Saudi Arabia between 1 April 2021, 30 2022. Nucleocapsid were through whole genome sequencing. 𝜒2 tests t assessed associations Logistic regression estimated risk of intensive care unit (ICU)...