A5101514354- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Plant biochemistry and biosynthesis
- Protist diversity and phylogeny
- Enzyme Catalysis and Immobilization
- Plant Gene Expression Analysis
- Plant Disease Resistance and Genetics
- Abdominal vascular conditions and treatments
- Click Chemistry and Applications
- Cancer Research and Treatments
- Lipid metabolism and biosynthesis
- Central Venous Catheters and Hemodialysis
- Genomics and Phylogenetic Studies
- Advanced Biosensing Techniques and Applications
- Cocoa and Sweet Potato Agronomy
- Legume Nitrogen Fixing Symbiosis
- Biological Stains and Phytochemicals
- Nitrogen and Sulfur Effects on Brassica
- Biofuel production and bioconversion
- Biochemical Acid Research Studies
- Botanical Research and Chemistry
Sweet Potato Research Institute
2022-2025
Nanyang Technological University
2025
Uppsala University
2018-2023
Northeast Forestry University
2018-2022
Chinese Academy of Agricultural Sciences
2022
Ministry of Agriculture and Rural Affairs
2022
Shanghai Normal University
2021
Third Hospital of Hebei Medical University
2020
Hebei Medical University
2020
China Pharmaceutical University
2012
Elevated temperatures limit plant growth and reproduction pose a growing threat to agriculture. Plant heat stress response is highly conserved fine-tuned in multiple pathways. Spinach (Spinacia oleracea L.) cold tolerant but sensitive green leafy vegetable. In this study, adaptation mechanisms spinach sibling inbred heat-tolerant line Sp75 were investigated using physiological, proteomic, phosphoproteomic approaches. The abundance patterns of 911 stress-responsive proteins, phosphorylation...
Cyanobacteria, oxygenic photoautotrophic prokaryotes, can be engineered to produce various valuable chemicals from solar energy and CO2 in direct processes. The concept of photosynthetic production isobutanol, a promising chemical drop-in biofuel, has so far been demonstrated for Synechocystis PCC 6803 Synechococcus elongatus 7942. In 6803, heterologous expression α-ketoisovalerate decarboxylase (Kivd) Lactococcus lactis resulted an isobutanol 3-methyl-1-butanol producing strain. Kivd was...
Protein engineering is a powerful tool to modify e.g. protein stability, activity and substrate selectivity. Heterologous expression of the enzyme α-ketoisovalerate decarboxylase (Kivd) in unicellular cyanobacterium Synechocystis PCC 6803 results cells producing isobutanol 3-methyl-1-butanol, with Kivd identified as potential bottleneck. In present study, we used improve production 6803. Isobutanol flammable compound that can be biofuel due its high energy density suitable physical chemical...
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a newly discovered lipid class known for their potential anti-inflammatory and insulin-sensitizing properties. A sustainable efficient synthesis route is essential to realize the FAHFAs enable cost-effective, large-scale production. Enzymatic synthesis, favored its scalability environmental impact, preferred approach. Candida (Moesziomyces) antarctica lipase (CalA), previously thermostability limited ability catalyze FAHFA esterification,...
Arctium lappa L. is a medicinal edible homologous plant, commonly known as burdock or bardana, which belongs to the Asteraceae family and abundant all over world. Genetic diversity assessment essential for A. germplasm resource conservation breeding. The techniques include morphological, biochemical, DNA marker analysis. However, limited number of available markers insufficient conduct related genetic studies. In this study, we conducted RNA sequencing cultivar 'Yanagikawa Ideal' developed...
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is an accurate and convenient method for mRNA quantification. Selection of optimal reference gene(s) important step in RT-qPCR experiments. However, the stability housekeeping genes spinach (Spinacia oleracea) under various abiotic stresses unclear. Evaluating candidate determining normalization gene expression are necessary to investigate patterns during development stress response. In this study, ten genes,...
Cyanobacteria are emerging as green cell factories for sustainable biofuel and chemical production, due to their photosynthetic ability use solar energy, carbon dioxide water in a direct process. The model cyanobacterial strain Synechocystis sp. PCC 6803 has been engineered the isobutanol 3-methyl-1-butanol production by introducing synthetic 2-keto acid pathway. However, achieved productions still remained low. In present study, diverse metabolic engineering strategies were implemented...
Stress-associated protein (SAP) genes-encoding A20/AN1 zinc-finger domain-containing proteins-play pivotal roles in regulating stress responses, growth, and development plants. They are considered suitable candidates to improve abiotic tolerance However, the SAP gene family sweetpotato (Ipomoea batatas) its relatives is yet be investigated. In this study, 20 SAPs sweetpotato, 23 26 wild diploid Ipomoea triloba trifida were identified. The chromosome locations, structures, physiological...
Abstract The photosynthetic cyanobacterium Synechocystis sp. PCC 6803 offers a promising sustainable solution for simultaneous CO 2 fixation and compound bioproduction. While various heterologous products have now been synthesised in , limited genetic tools hinder further strain engineering efficient production. Here, we present versatile CRISPR activation (CRISPRa) system enabling robust multiplexed of both endogenous targets. Following tool characterisation, applied CRISPRa to explore...
Cyanobacteria, photosynthetic microorganisms, are promising green cell factories for chemical production, including biofuels. Isobutanol, a four-carbon alcohol, is considered as superior candidate biofuel its high energy density with suitable and physical characteristics. The unicellular cyanobacterium Synechocystis PCC 6803 has been successfully engineered isobutanol production from CO2 solar in direct process.Heterologous expression of α-ketoisovalerate decarboxylase (KivdS286T) sufficient...
Doxorubicin (DOX) was conjugated to a single-chain variable fragment (scFv) against human midkine (MK), and the conjugate (scFv-DOX) used target chemotherapeutic agent mouse solid tumor model in which cells expressed high levels of MK. The His-tagged recombinant scFv bacteria, purified by metal affinity chromatography, then DOX using oxidative dextran (Dex) as linker. molecular formula this immunoconjugate scFv(Dex)1.3(DOX)20. In vitro apoptosis assays showed that scFv-DOX more cytotoxic...
Abstract Acyl-CoA-binding proteins (ACBPs) are important for the transport of acyl groups macromolecular biosynthesis involved in plant growth, development, and diverse stress (e.g., cold, drought, salinity, heavy metals) responses. Here, we report phylogeny characteristics ACBP family woody Populus trichocarpa . Eight genes encoding were identified, they distributed on eight chromosomes P. trichocarpa. These PtACBP divided into four subgroups according to gene structure, conserved motifs...