A5049735042- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Bone fractures and treatments
- Bone health and osteoporosis research
- Proteoglycans and glycosaminoglycans research
- Oceanographic and Atmospheric Processes
- Cytokine Signaling Pathways and Interactions
- Uterine Myomas and Treatments
- Ocean Waves and Remote Sensing
- Immune Cell Function and Interaction
- Endometriosis Research and Treatment
- MicroRNA in disease regulation
- Gynecological conditions and treatments
- Mesenchymal stem cell research
- Virus-based gene therapy research
- Glycosylation and Glycoproteins Research
- Bone Metabolism and Diseases
- Hip and Femur Fractures
- Estrogen and related hormone effects
- Cancer-related molecular mechanisms research
- Vitamin D Research Studies
- Climate variability and models
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
Nanjing Drum Tower Hospital
2012-2025
Nanjing University of Chinese Medicine
2024-2025
Qingdao University of Science and Technology
2023-2024
Institute of Oceanology
2023-2024
Chinese Academy of Sciences
2023-2024
Nanjing University
2009-2023
China Pharmaceutical University
2012-2023
Hainan Provincial Hospital of Traditional Chinese Medicine
2023
Nanjing Medical University
2011-2022
Bozhou People's Hospital
2020
Mesenchymal stem cells (MSCs) are attractive candidates for clinical therapeutic applications. Recent studies indicate MSCs express active Toll-like receptors (TLRs), but their effect on and the underlying mechanisms remain unclear. In this study, we found that, after treating human umbilical cord with various TLR ligands, only TLR3 ligand, poly(I:C), could significantly increase expression of cyclooxygenase-2 (COX-2). Furthermore, poly(I:C) enhance MSCs' anti-inflammatory macrophages. Next,...
Intrauterine adhesions (IUA), characterized by endometrial fibrosis, is a common cause of uterine infertility. We previously demonstrated that partial epithelial-mesenchymal transition (EMT) and the loss epithelial homeostasis play vital role in development fibrosis. As pro-survival strategy maintaining cell tissue homeostasis, macroautophagy/autophagy, conversely, may participate this process. However, autophagy fibrosis remains unknown. Here, we defective endometria IUA patients, which...
Abstract Macrophages are a key and heterogeneous cell population involved in endometrial repair regeneration during the menstrual cycle, but their role development of intrauterine adhesion (IUA) sequential fibrosis remains unclear. Here, we reported that CD301 + macrophages were significantly increased showed most active interaction with profibrotic cells endometria IUA patients compared normal by single‐cell RNA sequencing, bulk experimental verification. Increasing promoted differentiation...
Vaginal epithelial and smooth muscle cells were isolated expanded successfully. A collagen scaffold loaded with vaginal successfully reconstructed the vagina in a absence pig model.
Abstract Background Stanniocalcin-1(STC-1) is up-regulated in several cancers including gastric cancer. Evidences suggest that STC-1 associated with carcinogenesis and angiogenic process. However, it unclear on the exact role for inducing angiogenesis tumorigeneisis. Method BGC/STC cells (high-expression of STC-1) BGC/shSTC (low- expression were constructed to investigate effect xenograft tumor growth vitro vivo . ELISA assay was used detect vascular endothelial factor (VEGF) supernatants....
Macrophages exhibit a high degree of plasticity and play pivotal roles both in the normal physiological cycle endometrium its regeneration following injury. Although some new subsets endometrial macrophages have been identified, their origins functions remain to be further explored. In this study, we employed single-cell sequencing analyze patients with intrauterine adhesion (IUA) caused by We identified unique subset distinguished expression GATA6, marker indicative cavity macrophages....
Intrauterine adhesions (IUAs), also known as Asherman's syndrome (AS), represent a significant cause of uterine infertility for which effective treatment remains elusive. The endometrium's ability to regenerate cyclically depends heavily on the growth and regression its blood vessels. However, trauma endometrial basal layer can disrupt subepithelial capillary plexus, impeding regeneration. This damage results in replacement native cells with fibroblasts myofibroblasts, ultimately leading...
Problem Uterine natural killer cells (u NK ) do not express progesterone receptor, but glucocorticoid receptor ( GR ). So, we speculate that may regulate u through a ‐mediated process. Method of Study After mouse were stimulated with C p G or without IL ‐12 in the presence absence pre‐treatment progesterone, effects on via investigated by using RU 486 (progesterone and antagonist) CDB ‐2914 antagonist). The expressions CD 69 IFN ‐γ determined flow cytometry q PCR . Phosphorylation I κ B STAT...
In humans, a subset of placental cytotrophoblasts (CTBs) invades the uterus and its vasculature, anchoring pregnancy ensuring adequate blood flow to fetus. Appropriate depth is critical. Shallow invasion increases risk complications, e.g., severe preeclampsia. Overly deep invasion, hallmark placenta accreta spectrum (PAS), preterm delivery, hemorrhage, death. Previously rare condition, incidence PAS has increased 1:731 pregnancies, likely due rise in uterine surgeries (e.g., Cesarean...
Epithelial-mesenchymal transition (EMT) plays a specific role in the migration of tumor cells. Both estrogen and midkine (MK) have been thought to be important factors promoting progression non-small-cell lung cancer (NSCLC) can enhance EMT. Some evidence indicated correlation between estradiol (E2) MK, but precise mechanism on their interreaction is unknown. Here, we try clarify whether how E2 regulates MK expression promote We found that increased mRNA adenocarcinoma cells LTEP-a2 A549...
Abstract Intrauterine adhesions (IUAs), the leading cause of uterine infertility, are characterized by endometrial fibrosis. The management IUA is challenging because pathogenesis disease largely unknown. In this study, we demonstrate that mRNA and protein levels high mobility group AT-hook 2 (HMGA2) were increased nearly 3-fold (P < 0.0001) 5-fold = 0.0095) in epithelial cells (EECs) patients (n 18) compared to controls. vivo vitro models fibrosis also confirmed overexpression HMGA2...
Doxorubicin (DOX) was conjugated to a single-chain variable fragment (scFv) against human midkine (MK), and the conjugate (scFv-DOX) used target chemotherapeutic agent mouse solid tumor model in which cells expressed high levels of MK. The His-tagged recombinant scFv bacteria, purified by metal affinity chromatography, then DOX using oxidative dextran (Dex) as linker. molecular formula this immunoconjugate scFv(Dex)1.3(DOX)20. In vitro apoptosis assays showed that scFv-DOX more cytotoxic...
To investigate the effects of glossy ganoderma spore oil on proliferation, apoptosis, expression miR-21 and its target genes human lung adenocarcinoma SPC-A1 cell line, to explore possible mechanism.The cells were treated with for 24 48 hours. The inhibition growth efficacy was determined using count kit (CCK-8). Cell morphological changes observed by light microscopy. apoptosis analyzed flow cytometry. miR-21, PTEN PDCD4 Real-time PCR.Glossy concentration-dependently inhibited cell's...
A more comprehensive understanding of wave characteristics and their variability, based on reliable long-term data, is essential in the design, construction, operation management offshore coastal applications. This study aims to downscale multivariate data Chinese marginal seas at different time scales by employing two weather-type statistical downscaling models. The calibration both models involves usage historical from ERA5 reanalysis, with sea level pressure squared gradient as predictors...