A5102939142- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Pregnancy and preeclampsia studies
- Gynecological conditions and treatments
- Uterine Myomas and Treatments
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Ginseng Biological Effects and Applications
- Respiratory and Cough-Related Research
- Cancer, Hypoxia, and Metabolism
- Electrospun Nanofibers in Biomedical Applications
- Analytical chemistry methods development
- Eating Disorders and Behaviors
- Phytochemistry and biological activities of Ficus species
- Ion-surface interactions and analysis
- Neuroblastoma Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Diet and metabolism studies
- Adolescent and Pediatric Healthcare
- Mass Spectrometry Techniques and Applications
- Asthma and respiratory diseases
- Mesenchymal stem cell research
- Connective tissue disorders research
- Intestinal and Peritoneal Adhesions
Yale University
2023-2024
Nanjing Drum Tower Hospital
2018-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2012-2022
Hangzhou Medical College
2021
Southeast University
2018
Thin endometrium has been widely recognized as a critical cause of infertility, recurrent pregnancy loss, and placental abnormalities; however, access to effective treatment is formidable challenge due the rudimentary understanding pathogenesis thin endometrium. Here, we profiled transcriptomes human endometrial cells at single-cell resolution characterize cell types, their communications, underlying mechanism growth in normal during proliferative phase. Stromal were most abundant type...
Whole-body molecular imaging is able to directly map spatial distribution of molecules and monitor its biotransformation in intact biological tissue sections. Imaging mass spectrometry (IMS), a label-free method, can be used image multiple single measurement with high specificity. Herein, novel easy-to-implement, whole-body IMS method was developed air flow-assisted ionization desorption electrospray mode. The effectively large section open without sample pretreatment, such as chemical...
Intrauterine adhesions (IUA), characterized by endometrial fibrosis, is a common cause of uterine infertility. We previously demonstrated that partial epithelial-mesenchymal transition (EMT) and the loss epithelial homeostasis play vital role in development fibrosis. As pro-survival strategy maintaining cell tissue homeostasis, macroautophagy/autophagy, conversely, may participate this process. However, autophagy fibrosis remains unknown. Here, we defective endometria IUA patients, which...
Abstract Macrophages are a key and heterogeneous cell population involved in endometrial repair regeneration during the menstrual cycle, but their role development of intrauterine adhesion (IUA) sequential fibrosis remains unclear. Here, we reported that CD301 + macrophages were significantly increased showed most active interaction with profibrotic cells endometria IUA patients compared normal by single‐cell RNA sequencing, bulk experimental verification. Increasing promoted differentiation...
Intrauterine adhesion (IUA) is a common cause of uterine infertility and its histopathologic characteristic endometrial fibrosis. A shortage stem cells in the basalis has been recognized as IUA development because approximately 90% patients suffer from after injury. In this study, we provide evidence that persistent inflammation main contributor to fibrosis patients. We further found treating an IUA-like mouse model with ITI-hUC-MSCs (hUC-MSCs reprogrammed by IL-1β, TNF-α IFN-γ)...
Abstract Epithelial homeostasis plays an essential role in maintaining endometrial function. But the epithelial fibrosis has been less studied. Previously, we showed that ectopic expression of ΔNp63α is associated with process and dysfunction endometria patients intrauterine adhesions (IUAs). Since profoundly involved homeostasis, hereby focused on its roles regulating function phenotype cells (EECs) context fibrosis. We identified a typical type 2 epithelial-to-mesenchymal transition (EMT)...
Emerging evidence demonstrates the important role of circular RNAs (circRNAs) in regulating pathological processes various diseases including organ fibrosis. Endometrium fibrosis is leading cause uterine infertility, but circRNAs its pathogenesis largely unknown. Here, we provide that upregulation circPTPN12 endometrial epithelial cells (EECs) fibrotic endometrium functions as endogenous sponge miR-21–5 p to inhibit expression and activity, which turn results ΔNp63α induce mesenchymal...
The TET family of dioxygenases promote DNA demethylation by oxidizing 5-methylcytosine to 5-hydroxymethylcytosine (5hmC). Hypothalamic agouti-related peptide-expressing (AGRP-expressing) neurons play an essential role in driving feeding, while also modulating nonfeeding behaviors. Besides AGRP, these produce neuropeptide Y (NPY) and the neurotransmitter GABA, which act concert stimulate food intake decrease energy expenditure. Notably, NPY, GABA can elicit anxiolytic effects. Here, we report...
FAS-mediated apoptosis of hepatocytes and aberrant TGF-β signaling are major drivers liver fibrosis. Decreased miRNA let-7 expression in the livers patients animals with fibrosis suggests a mechanistic link to hepatic fibrogenesis. Using transient transfection we tested effects overexpression TET3 siRNA knockdown on FAS TGF-β1 human mouse primary hepatocytes. We assessed therapeutic activity delivered via adeno-associated viral vectors models carbon tetrachloride (CCl4)-induced bile duct...
Endometriosis is a debilitating, chronic inflammatory disease affecting approximately 10% of reproductive-age women worldwide with no cure. While macrophages have been intrinsically linked to the pathophysiology endometriosis, targeting them therapeutically has extremely challenging due their high heterogeneity and because these disease-associated (DAMs) can be either pathogenic or protective. Here, we report identification characterized by TET3 overexpression in human endometriosis lesions....
Thin endometrium (TE) is a challenging clinical issue in the reproductive medicine characterized by inadequate endometrial thickness, poor response to estrogen and no effective treatments currently. At present, precise pathogenesis of thin endometria remains be elucidated. We aimed explore related molecular mechanism TE comparing transcriptome profiles late-proliferative phase between matched controls.We performed bulk RNA-Seq (RNA-sequencing) tissues 7 controls for first time. Differential...
The aim of this study was to investigate the effects melatonin on oxidative stress, expression transient receptor potential melastatin-2 (TRPM2) in guinea pig brains, and influence stress lungs airway inflammation induced by particulate matter 2.5 (PM2.5). A particle suspension (0.1 g/ml) nasally administered pigs prepare a PM2.5 exposure model. Cough frequency cough incubation period were determined through RM6240B biological signal collection disposal system. Oxidative markers, including...
Preeclampsia (PE) is a pregnancy-related syndrome characterized by new-onset hypertension and proteinuria after gestational 20 weeks. Oxidative stress, resulting from the imbalance between production of oxidants antioxidants in placentas, recognized as key pathology PE. To date, molecules that regulate remain unclear. CD151, member tetraspanins, an important regulator many physiological functions. However, function CD151 oxidative stress its association with complications are currently...
Abstract Problem Asherman's syndrome (AS) is characterized by endometrial fibrosis leading to intrauterine adhesions and symptoms like hypomenorrhea, infertility, recurrent pregnancy loss. Macrophages are key regulators of inflammation, tissue repair, regeneration, fibrosis. However, the role macrophages in AS remains unclear. Method study Endometrial biopsies patients controls were collected during late proliferating phase menstrual cycle. Fibrosis proliferation markers detected Masson's...
Anorexia nervosa (AN) is a psychiatric illness with the highest mortality. Current treatment options have been limited to psychotherapy and nutritional support, low efficacy high relapse rates. Hypothalamic AgRP (agouti-related peptide) neurons that coexpress AGRP neuropeptide Y (NPY) play critical role in driving feeding while also modulating other complex behaviors. We previously reported genetic ablation of Tet3 , which encodes member TET family dioxygenases, specifically mice, activates...
Severe uterine injury is a major cause of endometrial scar formation and female infertility. At present, the methods for accelerating injured healing are still lacking. Genetic engineering modification mesenchymal stem cells (MSCs) has been shown great promise in preclinical studies on regeneration. Here, we constructed type umbilical cord MSCs (UC-MSCs) with overexpressed basic fibroblast growth factor (UCMSC-bFGF) investigated effects UCMSC-bFGF/scaffold functional regeneration...
Abstract Endometrial fibrosis is the main pathological feature of Asherman’s syndrome (AS), which leading cause uterine infertility. Much known about expression VEGF165 in luminal/glandular epithelial cells and stromal endometrium normal menstrual cycles; however, less role mechanism endometrial fibrosis. Herein, we report that a key regulator to inhibit α-SMA collagen 1 expression. Compared human control subjects, patients with AS exhibited decreased along increased fibrotic marker...
Preeclampsia is a complicated syndrome with marked heterogeneity. The biomarker-based classification for this more constructive to the targeted prevention and treatment of preeclampsia. It has been reported that preeclamptic patients had elevated microRNA-155 (miR-155) in placentas or circulation. Here, we investigated characteristics high placental miR-155 (pl-miR-155).Based on 95th percentile (P95) pl-miR-155 controls, were divided into group (≥P95) normal (<P95). changes pathology,...
Abstract Macrophages are a key and heterogeneous cell population involved in endometrial repair regeneration during the menstrual cycle, but their role development of intrauterine adhesion (IUA) sequential fibrosis remains unclear. Here, we reported that CD301+ macrophages were significantly increased showed most active interaction with profibrotic cells endometria IUA patients compared normal by single-cell RNA sequencing, bulk sequencing experimental verification. Increasing promoted...
Abstract Emerging evidence demonstrates the important role of circular RNAs (circRNAs) in regulating pathological processes various diseases including organ fibrosis. Endometrium fibrosis is leading cause uterine infertility, but circRNAs its pathogenesis largely unknown. Here, we provide that upregulation circPTPN12 endometrial epithelial cells (EECs) fibrotic endometrium functions as endogenous sponge miR-21-5p to inhibit expression and activity, which turn results ΔNp63α induce...