A5025114354- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Supramolecular Chemistry and Complexes
- Advanced Proteomics Techniques and Applications
- Traditional Chinese Medicine Analysis
- Advanced Chemical Sensor Technologies
- Natural product bioactivities and synthesis
- Cancer, Hypoxia, and Metabolism
- Chromatography in Natural Products
- Analytical chemistry methods development
- Supramolecular Self-Assembly in Materials
- Molecular Sensors and Ion Detection
- Ginseng Biological Effects and Applications
- Crystallography and molecular interactions
- Plant biochemistry and biosynthesis
- Phytochemical Studies and Bioactivities
- Ion-surface interactions and analysis
- Dendrimers and Hyperbranched Polymers
- Adipose Tissue and Metabolism
- Cancer, Lipids, and Metabolism
- Porphyrin and Phthalocyanine Chemistry
- Traditional Chinese Medicine Studies
- Phytochemistry and biological activities of Ficus species
- Microfluidic and Capillary Electrophoresis Applications
Minzu University of China
2016-2025
State Ethnic Affairs Commission
2021-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2024
Peking Union Medical College Hospital
2008-2020
Xinjiang Institute of Materia Medica
2017-2020
Academy of Medical Sciences
2020
Tsinghua University
2011
Peking University
2009
Hunan University
2009
Beijing National Laboratory for Molecular Sciences
2009
Characterization of tumor metabolism with spatial information contributes to our understanding complex cancer metabolic reprogramming, facilitating the discovery potential vulnerabilities that might be targeted for therapy. However, given variability and flexibility tumors, it is still challenging characterize global alterations in heterogeneous cancer. Here, we propose a spatially resolved metabolomics approach discover tumor-associated metabolites enzymes directly their native state. A...
By the introduction of trimethylammonium groups at both upper and lower rims, a cationic water-soluble pillar[5]arene was prepared, which forms stable 1 : host–guest complex with sodium 1-octanesulfonate in water.
Despite considerable efforts toward the development of various sophisticated spiropyrans for metal ion sensing, less attention has been paid to organic molecule sensing. One major difficulties detection molecules using a spiropyran is weak and nonspecific interaction between target. Here, we report synthesis molecular recognition characterization two bis-spiropyrans dipolar their application in vivo glutathione (GSH) fluorescent probes. Unlike mono-spiropyrans, newly designed bis-spiropyran...
Flavonol 3,7-di-O-glycosides were investigated by negative ion electrospray ionization tandem mass spectrometry using a quadrupole linear trap (LIT) spectrometer. The results indicate that the fragmentation behavior of flavonol is substantially different from their isomeric mono-O-diglycosides. In order to characterize flavonoid as 3,7-di-O-glycoside, both [Y3(0) - H]-* and [Y(0) 2H]- ions should be present in [M H]- product spectrum. MS(3) spectra Y3(0)-, Y7(0)- generated provide sufficient...
A metabonomics strategy based on rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS), multivariate statistics and metabolic correlation networks has been implemented to find biologically significant metabolite biomarkers in breast cancer. RRLC-MS/MS analysis by electrospray ionization (ESI) both positive negative ion modes was employed investigate human urine samples. The resulting data matrices were analyzed using analysis. Application of orthogonal projections...
A new water-soluble pillar[5]arene containing ten imidazolium groups was prepared. It can be used as a stabilizer to fabricate gold nanoparticles smaller than 6 nm in water.
Whole-body molecular imaging is able to directly map spatial distribution of molecules and monitor its biotransformation in intact biological tissue sections. Imaging mass spectrometry (IMS), a label-free method, can be used image multiple single measurement with high specificity. Herein, novel easy-to-implement, whole-body IMS method was developed air flow-assisted ionization desorption electrospray mode. The effectively large section open without sample pretreatment, such as chemical...
In this article, we present the design and construction of a series supramolecular poly(benzyl ether) metallodendrimers featuring well-defined hexagonal metallacycle at their cores via coordination-driven self-assembly. It was found that second generation metallodendrimer 3c able to hierarchically self-assemble into regular vesicle-like structures. These nanoscale vesicles were monodisperse in shape relatively size as detected SEM, TEM, AFM, DLS experiments. Notably, kind formed...
Histological examination with a deep link between functional metabolites and tissue structure biofunctions will provide important in situ biochemical information, then essentially reveal what has happened at the molecular level. However, due to complexity heterogeneity of samples large number metabolites, it is still challenge globally map diverse especially for those low-abundance ones. Here, sensitive air flow-assisted desorption electrospray ionization mass spectrometry imaging method...
Diagnostic and therapeutic biomarkers useful for esophageal squamous cell carcinoma (ESCC) have the ability to increase long term survival of cancer patients. A metabolomics study, using plasma from four groups including ESCC patients before, during, after chemoradiotherapy (CRT) healthy controls, was originally carried out by LC-MS determine global alterations in metabolic profiles find potentially applicable diagnosis monitoring treatment effects. It is worth pointing that a clear...
Detailed knowledge on tissue-specific metabolic reprogramming in diabetic nephropathy (DN) is vital for more accurate understanding the molecular pathological signature and developing novel therapeutic strategies. In present study, a spatial-resolved metabolomics approach based air flow-assisted desorption electrospray ionization (AFADESI) matrix-assisted laser (MALDI) integrated mass spectrometry imaging (MSI) was proposed to investigate alterations kidneys of high-fat diet-fed...
An integrated ionization approach of electrospray (ESI), atmospheric pressure chemical (APCI), and photoionization (APPI) combining with rapid resolution liquid chromatography mass spectrometry (RRLC−MS) has been developed for performing global metabonomic analysis on complex biological samples. This was designed to overcome the low efficiencies endogenous metabolites due diverse physicochemical properties as well ion suppression, obtain comprehensive metabolite profiles in LC−MS analysis....
Abstract The facile and efficient preparation of pillar[ n ]arenes ( = 5 or 6) was achieved by cyclooligomerization 2,5‐dialkoxybenzyl alcohols bromides with an appropriate Lewis acid catalyst at room temperature. mechanism for this is presumed to be a Friedel–Crafts alkylation.
Abstract Ambient ionization methods are an important research area in mass spectrometry (MS) analysis. Under ambient conditions, the gas flow and atmospheric pressure significantly affect transfer focusing of ions. The design implementation air assisted (AFAI) as a novel effective, remote sampling method for described herein. AFAI benefits from high extracting rate. A systematic investigation system has been carried out, it demonstrated not only that plays role effective capture transport...
The toxicities of polycyclic aromatic hydrocarbons (PAHs) have been extensively explored due to their carcinogenic and mutagenic potency; however, little is known about the metabolic responses chronic environmental PAH exposure among general population. In present study, 566 healthy volunteers were dichotomized into exposed control groups investigate PAH-induced perturbations in profiles. Nine urine metabolites measured by a sensitive LC-MS/MS method comprehensively evaluate level each...
By the introduction of 14 anionic carboxylate groups at its two rims, a water-soluble pillar[7]arene (WP7) was synthesized. Its pH-controlled complexation with paraquat G1 in water investigated. Host WP7 and guest formed 1:1 [2]pseudorotaxane high association constant (2.96 ± 0.31) × 10(9) M(-1) water. Furthermore, we took advantage this novel molecular recognition motif to fabricate supra-amphiphile based on an amphiphilic derivative G2. The morphologies sizes self-assemblies G2 WP7⊃G2 were...
We have combined droplet extraction and a pulsed direct current electrospray ionization mass spectrometry method (Pico-ESI-MS) to obtain information-rich metabolite profiling from single cells. studied normal human astrocyte cells glioblastoma cancer Over 600 tandem spectra (MS2) of metabolites cell were recorded, allowing the successful identification more than 300 phospholipids. found ratios unsaturated phosphatidylcholines (PCs) saturated PCs significantly higher in compared In addition,...
Elucidation of the mechanism action for drug candidates is fundamental to development, and it strongly facilitated by metabolomics. Herein, we developed an imaging metabolomics method based on air-flow-assisted desorption electrospray ionization mass spectrometry (AFADESI-MSI) under ambient conditions. This was subsequently applied simultaneously profile a novel anti-insomnia candidate, N6-(4-hydroxybenzyl)-adenosine (NHBA), various endogenous metabolites in rat whole-body tissue sections...
Abstract Sensitive and spatial exploration of the metabolism tumors at metabolome level is highly challenging. In this study, we developed an in situ metabolomics method based on ambient mass spectrometry imaging using air flow-assisted desorption electrospray ionization (AFADESI), which can spatially explore alteration global metabolites tissues with high sensitivity. Using method, discovered potential histopathological diagnosis biomarkers (including lipids, amino acids, choline, peptides...
Understanding of the nephrotoxicity induced by drug candidates is vital to discovery and development. Herein, an in situ metabolomics method based on air flow-assisted desorption electrospray ionization mass spectrometry imaging (AFADESI-MSI) was established for direct analysis metabolites renal tissue sections. This subsequently applied investigate spatially resolved metabolic profile changes rat kidney after administration aristolochic acid I, a known nephrotoxic drug, aimed discover...
Abstract Cardiovascular and metabolic disease (CMD) remains a main cause of premature death worldwide. Berberine (BBR), lipid-lowering botanic compound with diversified potency against disorders, is promising candidate for ameliorating CMD. The liver the target BBR so that liver-site accumulation could be important fulfilling its therapeutic effect. In this study rational designed micelle (CTA-Mic) consisting α-tocopheryl hydrophobic core on-site detachable polyethylene glycol-thiol shell...
Metabolic networks and their dysfunction in the brain are closely associated with central nervous function many psychogenic diseases. Thus, it is of utmost importance to develop a high-throughput imaging method for metabolic network mapping. Here, we developed mapping discover contexts alterations spatially resolved information from microregion by ambient-air flow-assisted desorption electrospray ionization mass spectrometry metabolomics analysis, which can be performed without any chemical...
Diabetes cardiomyopathy (DCM) is a metabolic disease and leading cause of heart failure among people with diabetes. Mass spectrometry imaging (MSI) versatile technique capable combining the molecular specificity mass spatial information imaging. In this study, we used MSI to visualize metabolites in rat high resolution sensitivity. We optimized air flow-assisted desorption electrospray ionization (AFADESI)-MSI platform detect wide range metabolites, then matrix-assisted laser (MALDI)-MSI for...
Metabonomics is an important platform for investigating the metabolites of integrated living systems and their dynamic responses to changes caused by both endogenous exogenous factors. A metabonomics strategy based on liquid chromatography-mass spectrometry/mass spectrometry in positive negative ion modes was applied investigate short-term long-term stability plasma. Principal components analysis ten types identified were used summarize time-dependent change rules systematically at different...