A5046967677- Metabolomics and Mass Spectrometry Studies
- Phytochemistry and Biological Activities
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Single-cell and spatial transcriptomics
- Natural product bioactivities and synthesis
- Fungal Biology and Applications
- Drilling and Well Engineering
- Essential Oils and Antimicrobial Activity
- Cell Image Analysis Techniques
- Immune Response and Inflammation
- Cancer, Lipids, and Metabolism
- Herbal Medicine Research Studies
- Cancer-related molecular mechanisms research
- Tunneling and Rock Mechanics
- Lipid metabolism and biosynthesis
- Phytochemicals and Antioxidant Activities
- Milk Quality and Mastitis in Dairy Cows
- Plant Genetic and Mutation Studies
- Signaling Pathways in Disease
- NF-κB Signaling Pathways
- Analytical chemistry methods development
- Genetics and Neurodevelopmental Disorders
- Silymarin and Mushroom Poisoning
- Granular flow and fluidized beds
Texas A&M University
2024
Yangzhou University
2024
Southwest Petroleum University
2023-2024
Yanbian University
2023
Tonghua Normal University
2023
Tsinghua University
2018-2023
Tianjin First Center Hospital
2021-2023
Tianjin Medical University
2021-2023
South China University of Technology
2021
Northwest A&F University
2018-2021
Lipids play a pivotal role in biological processes and lipid analysis by mass spectrometry (MS) has significantly advanced lipidomic studies. While the structure specificity of proves to be critical for studying functions lipids, current mainstream methods large-scale can only identify classes fatty acyl chains, leaving C=C location sn-position unidentified. In this study, combining photochemistry tandem MS we develop simple but effective workflow enable near-complete characterization with...
Abstract Objectives The correlations between long non‐coding RNAs (lncRNAs) and diverse mammal diseases have been clarified by many researches, but the cognition about bovine mastitis‐related lncRNAs remains limited. This study aimed to investigate potential role of lncRNA X‐inactive specific transcript (XIST) in inflammatory response mammary epithelial cells. Materials methods Two alveolar cell‐T (MAC‐T) models were established infecting cells with Escherichia coli (E. coli) Staphylococcus...
Comprehensive analysis of single-cell metabolites is critical since differences in cellular chemical compositions give rise to specialized biological functions. Herein, we propose a label-free mass cytometry by coupling flow ESI-MS (named CyESI-MS) for high-coverage and high-throughput detection metabolites. Cells suspension were isolated, online extracted sheath fluid, lysed during gas-assisted electrospray, followed real-time MS analysis. Hundreds metabolites, including nucleotides, amino...
We have combined droplet extraction and a pulsed direct current electrospray ionization mass spectrometry method (Pico-ESI-MS) to obtain information-rich metabolite profiling from single cells. studied normal human astrocyte cells glioblastoma cancer Over 600 tandem spectra (MS2) of metabolites cell were recorded, allowing the successful identification more than 300 phospholipids. found ratios unsaturated phosphatidylcholines (PCs) saturated PCs significantly higher in compared In addition,...
Changes of metabolite concentrations in single cells are significant for exploring the dynamic regulation important biological processes, such as cell development and differentiation. Accurate quantitation metabolites is essential analysis. In this work, we proposed a quantitative method single-cell by combining microwell array with droplet microextraction-mass spectrometry. The can confine both extraction solvent defined space, avoiding irregular spread trace internal standard solution...
The disruption of mitochondrial redox homeostasis in endothelial cells (ECs) can cause chronic inflammation, a substantial contributor to the development atherosclerosis. Chronic sympathetic hyperactivity enhance oxidative stress induce dysfunction. We determined if renal denervation (RDN), strategy reducing tone, protect ECs by ameliorating reactive oxygen species (ROS)-induced inflammation reduce ApoE deficient (ApoE−/-) mice were conducted RDN or sham operation before 20-week high-fat...
The study of lipid metabolism relies on the characterization lipidome, which is quite complex due to structure variations species. New analytical tools have been developed recently for characterizing fine structures lipids, with C=C location identification as one major improvements. In this study, we studied reprograming by analyzing glycerol phospholipid compositions in breast cancer cell lines structural specification extended level. Inhibition desaturase, stearoyl-CoA desaturase 1,...
Microplastics (MPs) are universally present in the ecosystem and pose great threats to environment living organisms. Research studies have shown that small MPs (<50 μm diameter) especially toxic account for more than half of all collected Atlantic Ocean. Nevertheless, current methods detection analysis incapable achieving rapid situ biota ultimately enable study their biological effects. In this work, we report a method allows identification spatial mapping directly from paramecia with high...
Changes in the levels of lipid
Maslinic acid (MA) is a pentacyclic triterpene obtained from the peel of olives that exhibits anti-inflammatory and antioxidant properties in several conditions. Our previous study revealed MA exerted cardioprotective effect by repressing inflammation apoptosis during myocardial ischemia-reperfusion injury (MIRI). However, data regarding antioxidative effects on MIRI remains limited. This aims to elucidate roles underlying mechanisms MIRI. The left anterior descending coronary artery rats...
MicroRNA-199a-5p (miR-199a-5p) and -3p are enriched in the myocardium, but it is unknown whether miR-199a-5p co-expressed cardiac remodeling what roles they have hypertrophy fibrosis. We show that co-upregulated mouse human myocardium with Ang-II-treated neonatal ventricular cardiomyocytes (NMVCs) fibroblasts (CFs). could aggravate fibrosis vivo vitro. PPAR gamma coactivator 1 alpha (Ppargc1a) sirtuin (Sirt1) were identified as target genes to mediate promoting both However, miR-199a-3p...
Aims: The inflammatory response and apoptosis are the major pathological features of myocardial ischemia/reperfusion injury (MI/RI). Maslinic acid (MA), a natural pentacyclic triterpene with various bioactivities, plays critical roles in multiple cellular biological processes, but its protective effects on pathophysiological processes MI/RI have not been extensively investigated. Our study aimed to determine whether MA treatment alleviate (I/R)-induced inflammation both vitro vivo, further...
Discriminating various leukocyte subsets with specific functions is critical due to their important roles in the development of many diseases. Here, we proposed a general strategy unravel leukocytes heterogeneity and screen differentiated metabolites as biomarker candidates for subtypes using label-free mass cytometry (CyESI-MS) combined homemade data processing workflow. Taking leukemia cells an example, metabolic fingerprints single were obtained from 472 HL-60, 416 THP-1, 313 U937, 356...
Euphorbia fischeriana has a long-standing history of use in traditional medicine for the treatment tuberculosis diseases. However, plant’s therapeutic potential extends beyond this specific ailment. The present study aimed to investigate antioxidant properties and lay groundwork further research on its applications. Phytochemical tests were performed plant, 11 types phytochemicals identified. Ultraviolet–visible spectrophotometry was used evaluate active components eight different solvent...
Laportea bulbifera (Sieb. et Zucc.) Wedd., a plant with long history of medicinal use, possesses uncertainly defined medicament portions while its antioxidant capacity remains largely unexplored. To gain better understanding value, this study focused on investigating the aboveground part (LBAP) and root (LBR). Through an assessment bioactive compound content, significant finding emerged: LBR exhibited notably higher levels these phytochemicals compared to LBAP. This observation was further...
<i>Objective</i>: The increasing demand for unraveling cellular heterogeneity has boosted single cell metabolomics studies. However, current analytical methods are usually labor-intensive and hampered by lack of accuracy efficiency. <i>Methods</i>: we developed a first-ever automated mass spectrometry system (named SCMS) that facilitated the metabolic profiling cells. In particular, extremely small droplets sub nano-liter were generated to extract cells, underlying mechanism was verified...
While studies of collective cell migration and bacteria swarming have tremendously promoted our fundamental knowledge the complex systematic phenomena, quantitative characterization organelles movement at subcellular level is yet to be fully explored. Here we tagged lysosomes in live cells with fluorescent probe imaged their spatial motion wide field microscopy. To quantitatively characterize lysosomal behavior high spatiotemporal heterogeneity dynamics, developed particle analysis (PECAN)...
Omega-3 polyunsaturated fatty acids (n-3 PUFAs), essential for humans and animals, have been reported to play a beneficial role in variety of inflammatory diseases. In this study, we investigated the inhibitory effects potential molecular mechanisms n-3 PUFAs on response lipopolysaccharide (LPS)-stimulated mammary alveolar cell line (MAC-T). Results showed that could abate LPS-induced secretions tumor necrosis factor-α, interleukin (IL)-6 IL-1β MAC-T cells through nuclear transcription...