A5100605594- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Child and Adolescent Psychosocial and Emotional Development
- Advanced Chemical Sensor Technologies
- Cancer, Hypoxia, and Metabolism
- Advanced Proteomics Techniques and Applications
- Traditional Chinese Medicine Analysis
- MicroRNA in disease regulation
- Chromatography in Natural Products
- Epigenetics and DNA Methylation
- Vitamin D Research Studies
- Psychological Well-being and Life Satisfaction
- Medical Research and Treatments
- Ginseng Biological Effects and Applications
- Impact of Technology on Adolescents
- Early Childhood Education and Development
- Genomics and Rare Diseases
- Microbial Metabolism and Applications
- Genetics and Neurodevelopmental Disorders
- Adipose Tissue and Metabolism
- Plant Reproductive Biology
- Bullying, Victimization, and Aggression
- Microfluidic and Capillary Electrophoresis Applications
- Analytical chemistry methods development
Shanxi Medical University
2009-2025
Tianjin Children's Hospital
2019-2024
China-Japan Friendship Hospital
2015-2024
Tianjin University
2023-2024
Zhengzhou University
2015-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2023
Tianjin Beichen Hospital
2023
Tianjin Medical University
2017-2023
Shanxi Academy of Medical Sciences
2023
State Ethnic Affairs Commission
2023
Characterization of tumor metabolism with spatial information contributes to our understanding complex cancer metabolic reprogramming, facilitating the discovery potential vulnerabilities that might be targeted for therapy. However, given variability and flexibility tumors, it is still challenging characterize global alterations in heterogeneous cancer. Here, we propose a spatially resolved metabolomics approach discover tumor-associated metabolites enzymes directly their native state. A...
Flavonol 3,7-di-O-glycosides were investigated by negative ion electrospray ionization tandem mass spectrometry using a quadrupole linear trap (LIT) spectrometer. The results indicate that the fragmentation behavior of flavonol is substantially different from their isomeric mono-O-diglycosides. In order to characterize flavonoid as 3,7-di-O-glycoside, both [Y3(0) - H]-* and [Y(0) 2H]- ions should be present in [M H]- product spectrum. MS(3) spectra Y3(0)-, Y7(0)- generated provide sufficient...
A metabonomics strategy based on rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS), multivariate statistics and metabolic correlation networks has been implemented to find biologically significant metabolite biomarkers in breast cancer. RRLC-MS/MS analysis by electrospray ionization (ESI) both positive negative ion modes was employed investigate human urine samples. The resulting data matrices were analyzed using analysis. Application of orthogonal projections...
Whole-body molecular imaging is able to directly map spatial distribution of molecules and monitor its biotransformation in intact biological tissue sections. Imaging mass spectrometry (IMS), a label-free method, can be used image multiple single measurement with high specificity. Herein, novel easy-to-implement, whole-body IMS method was developed air flow-assisted ionization desorption electrospray mode. The effectively large section open without sample pretreatment, such as chemical...
Vitamin D deficiency, which is usually detected by using immunoassays or the more reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) methods, has recently been considered a public health problem worldwide. However, vitamin status in Chinese populations, as measured LC-MS/MS method, not available. The objective of this multicenter study was to determine and prevalence deficiency method 5 large cities China. From May 1 September 31, 2013, we conducted on 2173 apparently healthy...
Diagnostic and therapeutic biomarkers useful for esophageal squamous cell carcinoma (ESCC) have the ability to increase long term survival of cancer patients. A metabolomics study, using plasma from four groups including ESCC patients before, during, after chemoradiotherapy (CRT) healthy controls, was originally carried out by LC-MS determine global alterations in metabolic profiles find potentially applicable diagnosis monitoring treatment effects. It is worth pointing that a clear...
An integrated ionization approach of electrospray (ESI), atmospheric pressure chemical (APCI), and photoionization (APPI) combining with rapid resolution liquid chromatography mass spectrometry (RRLC−MS) has been developed for performing global metabonomic analysis on complex biological samples. This was designed to overcome the low efficiencies endogenous metabolites due diverse physicochemical properties as well ion suppression, obtain comprehensive metabolite profiles in LC−MS analysis....
Abstract Ambient ionization methods are an important research area in mass spectrometry (MS) analysis. Under ambient conditions, the gas flow and atmospheric pressure significantly affect transfer focusing of ions. The design implementation air assisted (AFAI) as a novel effective, remote sampling method for described herein. AFAI benefits from high extracting rate. A systematic investigation system has been carried out, it demonstrated not only that plays role effective capture transport...
The toxicities of polycyclic aromatic hydrocarbons (PAHs) have been extensively explored due to their carcinogenic and mutagenic potency; however, little is known about the metabolic responses chronic environmental PAH exposure among general population. In present study, 566 healthy volunteers were dichotomized into exposed control groups investigate PAH-induced perturbations in profiles. Nine urine metabolites measured by a sensitive LC-MS/MS method comprehensively evaluate level each...
We have combined droplet extraction and a pulsed direct current electrospray ionization mass spectrometry method (Pico-ESI-MS) to obtain information-rich metabolite profiling from single cells. studied normal human astrocyte cells glioblastoma cancer Over 600 tandem spectra (MS2) of metabolites cell were recorded, allowing the successful identification more than 300 phospholipids. found ratios unsaturated phosphatidylcholines (PCs) saturated PCs significantly higher in compared In addition,...
Elucidation of the mechanism action for drug candidates is fundamental to development, and it strongly facilitated by metabolomics. Herein, we developed an imaging metabolomics method based on air-flow-assisted desorption electrospray ionization mass spectrometry (AFADESI-MSI) under ambient conditions. This was subsequently applied simultaneously profile a novel anti-insomnia candidate, N6-(4-hydroxybenzyl)-adenosine (NHBA), various endogenous metabolites in rat whole-body tissue sections...
A large body of literature has examined the relations between social support and depression. However, exact nature direction these are not well understood. This study explored specific types (peer teacher support) Adolescents (ages 11 to 17) for first time (N = 2453) participated in a two-wave, 6-month longitudinal study. Structural equation modeling was used test causation model (deficits increase likelihood depression), interpersonal theories depression (depression leads erosion),...
Metabolic networks and their dysfunction in the brain are closely associated with central nervous function many psychogenic diseases. Thus, it is of utmost importance to develop a high-throughput imaging method for metabolic network mapping. Here, we developed mapping discover contexts alterations spatially resolved information from microregion by ambient-air flow-assisted desorption electrospray ionization mass spectrometry metabolomics analysis, which can be performed without any chemical...
Metabonomics is an important platform for investigating the metabolites of integrated living systems and their dynamic responses to changes caused by both endogenous exogenous factors. A metabonomics strategy based on liquid chromatography-mass spectrometry/mass spectrometry in positive negative ion modes was applied investigate short-term long-term stability plasma. Principal components analysis ten types identified were used summarize time-dependent change rules systematically at different...
It is essential to choose one preprocessing method for liquid chromatography-mass spectrometry (LC-MS)-based metabolomics studies of urine samples in order overcome their variability. However, the commonly used normalization methods do not substantially reduce high variabilities arising from differences concentration, especially signal saturation (abundant metabolites exceed dynamic range instrumentation) or missing values. Herein, a simple preacquisition strategy based on differential...
Quantitative metabolomics approaches can significantly improve the repeatability and reliability of investigations but face critical technical challenges, owing to vast number unknown endogenous metabolites lack authentic standards. The present study contributes development a novel method known as "data-independent targeted quantitative metabolomics" (DITQM), which was used investigate label-free multiple in biofluid samples. This approach initially involved acquisition MS/MS data for all...
The development of improved or targeted drugs that discriminate between normal and tumor tissues is the key therapeutic issue in cancer research.However, an analytical method with a high accuracy sensitivity to achieve quantitative assessment targeting anticancer even intratumor heterogeneous distribution these at early stages drug research major challenge.Mass spectrometry imaging label-free molecular technique provides spatial-temporal information on metabolites organisms, its application...
It is highly challenging to quantitatively map multiple analytes in biotissues without specific chemical labeling. Quantitative mass spectrometry imaging (QMSI) has this potential but still poses technical issues for its variant ionization efficiency across a complicated, heterogeneous biomatrices. Herein, self-developed air-flow-assisted desorption electrospray (AFADESI) introduced present proof of concept method, virtual calibration (VC) QMSI. This method screens and utilizes analyte...