A5100649680- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Natural product bioactivities and synthesis
- Cancer therapeutics and mechanisms
- Cancer Treatment and Pharmacology
- Synthesis and biological activity
- Quinazolinone synthesis and applications
- Bioactive Compounds and Antitumor Agents
- Phytochemistry and Biological Activities
- Microbial Natural Products and Biosynthesis
- Phytochemical compounds biological activities
- PI3K/AKT/mTOR signaling in cancer
- Peptidase Inhibition and Analysis
- Natural Compounds in Disease Treatment
- Traditional and Medicinal Uses of Annonaceae
- Cancer, Hypoxia, and Metabolism
- PARP inhibition in cancer therapy
- Sphingolipid Metabolism and Signaling
- Synthetic Organic Chemistry Methods
- Computational Drug Discovery Methods
- Marine Sponges and Natural Products
- Chronic Lymphocytic Leukemia Research
- Phytochemistry and Bioactive Compounds
- Histone Deacetylase Inhibitors Research
- Cytokine Signaling Pathways and Interactions
China University of Petroleum, East China
2025
Henan University of Science and Technology
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2024
Peking Union Medical College Hospital
2008-2023
Academia Sinica
2022-2023
Academy of Medical Sciences
1999-2023
Changchun University of Technology
2023
Donghua University
2022-2023
Macau University of Science and Technology
2022
Animal Science Research Institute
2022
Abstract The present study investigates the induction of neurogenesis, reduction apoptosis, and promotion basic fibroblast growth factor (bFGF) expression as possible mechanisms by which treatment stroke with bone marrow stromal cells (MSCs) improves neurological functional recovery. Additionally, for first time, we treated cerebral ischemia in female rats intraveneous administration MSCs. Female were subjected to 2 hr middle artery occlusion (MCAo), followed an injection 3 × 10 6 male (for...
The amino acid antiporter system Xc- is important for the synthesis of glutathione (GSH) that functions to prevent lipid peroxidation and protect cells from nonapoptotic, iron-dependent death (i.e., ferroptosis). While activity often positively correlates with expression level its light chain encoded by SLC7A11, inhibition small molecules (e.g., erastin) causes a decrease in intracellular GSH level, leading ferroptotic cell death. How regulated during ferroptosis remains largely unknown....
Intravenous administration of human bone marrow stromal cells (hMSCs) after middle cerebral artery occlusion (MCAo) in rats provides functional benefit. We tested the hypothesis that these benefits are derived part from hMSC production growth and trophic factors. Quantitative sandwich enzyme‐linked immunosorbent assay (ELISA) hMSCs cultured with normal MCAo brain extracts were performed. supernatant ischemic increased brain‐derived neurotrophic factor (BDNF), nerve (NGF), vascular...
Abstract Treatment of traumatic brain injury (TBI) with bone marrow stromal cells (MSCs) improves functional outcome in the rat. However, specific mechanisms by which introduced MSCs provide benefit remain to be elucidated. Currently, ability therapeutically transplanted replace injured parenchymal CNS tissue appears limited at best. Tissue replacement, however, is not only possible compensatory avenue cell transplantation therapy. Various growth factors have been shown mediate repair and...
Bone marrow stromal cells (MSCs) administered intravenously are effective in reducing neurological deficits after stroke the rodent. These appear to selectively migrate and express neural phenotypes ischemic brain. To elucidate mechanisms targeting MSC migration into brain, we measured, using a microchemotaxis chamber, effect of select chemotactic factors cytokines expressed injured monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1α (MIP-1α) interleukin-8 (IL-8),...
Abstract Epithelial-to-mesenchymal transition (EMT), a crucial developmental program, contributes to cancer invasion and metastasis. In this study, we show that death-effector domain-containing DNA-binding protein (DEDD) attenuates EMT acts as an endogenous suppressor of tumor growth We found expression levels DEDD were conversely correlated with poor prognosis in patients breast colon cancer. Both vitro vivo, overexpression attenuated the invasive phenotype highly metastatic cells, whereas...
Abstract Glioblastoma is an aggressive tumor that associated with distinctive infiltrating microglia/macrophages populations. Previous studies demonstrated chlorogenic acid (5-caffeoylquinic acid, CHA), a phenolic compound low molecular weight, has anti-tumor effect in multiple malignant tumors. In the present study, we focused on macrophage polarization to investigate mechanisms behind anti-glioma response of CHA vitro and vivo . We found treatment increased expression M1 markers induced by...
Tribbles homolog 3 (TRB3, also known as TRIB3, NIPK and SKIP3), a human of Drosophila Tribbles, has been found to interact with variety signaling molecules regulate diverse cellular functions. Here, we report that TRB3 is novel SMAD3-interacting protein. Expression exogenous enhanced the transcriptional activity SMAD3, whereas knocking down endogenous reduced SMAD3. The kinase-like domain (KD) was responsible for interaction SMAD3 regulation SMAD3-mediated activity. In addition, TGF-β1...
Whole-body molecular imaging is able to directly map spatial distribution of molecules and monitor its biotransformation in intact biological tissue sections. Imaging mass spectrometry (IMS), a label-free method, can be used image multiple single measurement with high specificity. Herein, novel easy-to-implement, whole-body IMS method was developed air flow-assisted ionization desorption electrospray mode. The effectively large section open without sample pretreatment, such as chemical...
Seven new indole alkaloids, bruceollines H–N (1–7), three quassinoids, yadanziolides T–V (10–12), and four known analogues, bruceolline E (8), F (9), bruceine D (13), yadanziolide B (14), were isolated from an ethanol extract of the stems Brucea mollis. The absolute configurations compounds 2 5 determined by comparison their experimental calculated ECD spectra. configuration compound 9 was using Mo2(OAc)4-induced CD analysis for first time. Compounds 10, 13, 14 exhibited cytotoxic activities...
Tumor-associated macrophages (TAMs) generally display an immunosuppressive M2 phenotype and promote tumor progression metastasis, suggesting their potential value as a target in cancer immunotherapy. Chlorogenic acid (CHA) has been identified potent immunomodulator that promotes the polarization of TAMs from to M1 phenotype. However, rapid clearance vivo low accumulation have compromised immunotherapeutic efficacy CHA clinical trials. In this study, mannosylated liposomes are developed for...
Skin aging is categorized as chronological and photo-aging that affected by intrinsic extrinsic factors. The present study aimed to investigate the anti-aging ability its underlying mechanism of chlorogenic acid (CGA) on human dermal fibroblasts (HDFs). In this study, CGA specifically up-regulated collagen I (Col1) mRNA protein expressions increased secretion in supernatant HDFs without affecting cell viability, latter was also demonstrated BioMAP HDF3CGF system. Under ultraviolet A...
Chlorogenic acid (CGA) is a natural product that effectively inhibits tumor growth, demonstrated in many preclinical models, and phase II clinical trials for patients with glioma. However, its direct proteomic targets anticancer molecular mechanisms remain unknown. Herein, we developed novel bi-functional photo-affinity probe PAL/CGA discovered mitochondrial acetyl-CoA acetyltransferase 1 (ACAT1) was one of the main target proteins CGA by using affinity-based protein profiling (AfBPP)...
Three unique pyrano[4,3-c][2]benzopyran-1,6-dione derivatives and a new furo[3,2-c]pyran-4-one, named phelligridins C-F (2-5), together with hispolon (8), (E)-4-(3,4-dihydroxyphenyl)but-3-en-2-one (9), 4-hydroxybenzaldehyde, protocatechualdehyde, syringic acid, protocatechuic caffeic isoergosterone, octadecyl ferulate were isolated identified from the ethanolic extract of Phellinus igniarius. Their structures determined by spectroscopic methods including IR, MS, 1D 2D NMR experiments. The...
Treatment of rodents after stroke with bone marrow stromal cells (BMSCs) improves functional outcome. However, the mechanisms underlying this benefit have not been ascertained. This study focused on contribution neurotrophic and growth factors produced by BMSCs to therapeutic benefit. Rats were subjected middle cerebral artery occlusion ischemic brain extract supernatant was collected prepare conditioned medium. The counterpart normal from non‐ischemic rats employed as experimental control....
Thirteen new thiodiketopiperazines, epicoccin I (1), ent-epicoccin G (2), and epicoccins J−T (3−13), together with six known diketopiperazines (14−19), have been isolated from the endophytic fungus Epicoccum nigrum. The structures of 1, 2, 10 were confirmed by X-ray crystallography, absolute configurations 4, 6, 8 assigned using Moshers' method. Compounds 12, 17 showed potent activities in vitro against release β-glucuronidase rat polymorphonuclear leukocytes induced platelet-activating...
Bone marrow stromal cells (MSCs) improve neurologic recovery after middle cerebral artery occlusion (MCAo). To examine whether in vivo blockage of the endogenous sonic hedgehog (Shh) pathway affects grafted MSC-induced benefits, MCAo mice were administered: vehicle (control); cyclopamine (CP)- a specific Shh inhibitor; MSC; and MSC (MSC-CP). Neurologic function was evaluated MCAo. Electron microscopy immunofluorescence staining employed to measure synapse density, protein expression tissue...
Polypharmacology is a promising paradigm in modern drug discovery. Herein, we have discovered series of novel PI3K and HDAC dual inhibitors which the hydroxamic acid moiety as zinc binding functional group was introduced to quinazoline-based pharmacophore through an appropriate linker. Systematic structure–activity relationship studies resulted lead compounds 23 36 that simultaneously inhibited with nanomolar potencies demonstrated favorable antiproliferative activities. Compounds...
Background Septic acute kidney injury (AKI) causes high mortality in critical care units, and no effective therapy exists clinical treatment. In the current study, water ethanol extracts of Hydrangea paniculata (HP), a traditional Chinese medicinal plant, were used to test its renoprotective effects lipopolysaccharide (LPS)-induced murine model septic AKI. Methods C57BL/6 mice orally pretreated with HP three times, then intraperitoneal LPS injection was induce Blood from animals collected...
A general and practical protocol was developed for the regioselective C-H azolation of phenol aniline derivatives by electrooxidative cross-coupling. The reaction occurs under metal-, oxidant-, reagent-free conditions, allowing access to a wide variety synthetically useful heteroarene derivatives. also tolerates broad range functional groups is amenable gram-scale synthesis. Finally, preliminary mechanistic study indicated that radical-radical-combination pathway might be involved in...