A5100435914- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Nerve injury and regeneration
- Bone Tissue Engineering Materials
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA modifications and cancer
- Organ Transplantation Techniques and Outcomes
- Lung Cancer Treatments and Mutations
- Vascular Malformations and Hemangiomas
- Extracellular vesicles in disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenetic and Muscular Disorders Research
- Neonatal Respiratory Health Research
- Periodontal Regeneration and Treatments
- Oral and gingival health research
- Ear and Head Tumors
- Tumors and Oncological Cases
- Bone Metabolism and Diseases
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Vascular Malformations Diagnosis and Treatment
Air Force Medical University
2012-2024
Kunming Medical University
2023
Academy of Military Medical Sciences
2022
BGI Group (China)
2019-2020
China National GeneBank
2019-2020
Shanghai Jiao Tong University
2016-2020
Air Force Engineering University
2020
Shanghai Ninth People's Hospital
2016-2020
First People's Hospital of Yunnan Province
2020
Kunming University of Science and Technology
2020
Background and Purpose —We tested the hypothesis that intravenous infusion of bone marrow derived–marrow stromal cells (MSCs) enter brain reduce neurological functional deficits after stroke in rats. Methods —Rats (n=32) were subjected to 2 hours middle cerebral artery occlusion (MCAO). Test groups consisted MCAO alone (group 1, n=6); 1×10 6 MSCs at 24 2, or 3×10 3, n=7). Rats 1 3 euthanized 14 days MCAO. Group 4 (n=6) group 5, 7 (n=7). 5 35 For cellular identification, prelabeled with...
We tested the hypothesis that intravenous infusion of human bone marrow stromal cells (hMSCs) promotes vascular endothelial growth factor (VEGF) secretion, VEGF receptor 2 (VEGFR2) expression and angiogenesis in ischemic boundary zone (IBZ) after stroke. hMSCs (1×10 6 ) were intravenously injected into rats 24 hours middle cerebral artery occlusion (MCAo). Laser scanning confocal microscopy (LSCM), immunohistochemistry ELISA performed to assay levels rat host brain, respectively. In...
The authors transplanted adult bone marrow nonhematopoietic cells into the striatum after embolic middle cerebral artery occlusion (MCAO). Mice (n = 23; C57BL/6J) were divided four groups: (1) mice 5) subjected to MCAO and with (prelabeled by bromodeoxyuridine, BrdU) ischemic striatum, (2) alone 8), (3) injection of phosphate buffered saline 5), (4) injected normal 5). killed at 28 days stroke. BrdU reactive survived migrated a distance approximately 2.2 mm from grafting areas toward areas....
Intravenous administration of human bone marrow stromal cells (hMSCs) after middle cerebral artery occlusion (MCAo) in rats provides functional benefit. We tested the hypothesis that these benefits are derived part from hMSC production growth and trophic factors. Quantitative sandwich enzyme‐linked immunosorbent assay (ELISA) hMSCs cultured with normal MCAo brain extracts were performed. supernatant ischemic increased brain‐derived neurotrophic factor (BDNF), nerve (NGF), vascular...
Abstract Treatment of traumatic brain injury (TBI) with bone marrow stromal cells (MSCs) improves functional outcome in the rat. However, specific mechanisms by which introduced MSCs provide benefit remain to be elucidated. Currently, ability therapeutically transplanted replace injured parenchymal CNS tissue appears limited at best. Tissue replacement, however, is not only possible compensatory avenue cell transplantation therapy. Various growth factors have been shown mediate repair and...
Bone marrow stromal cells (MSCs) administered intravenously are effective in reducing neurological deficits after stroke the rodent. These appear to selectively migrate and express neural phenotypes ischemic brain. To elucidate mechanisms targeting MSC migration into brain, we measured, using a microchemotaxis chamber, effect of select chemotactic factors cytokines expressed injured monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1α (MIP-1α) interleukin-8 (IL-8),...
This study assessed the potential therapeutic efficacy of adipose-derived stem cells (ASCs) on infarcted hearts. Myocardial infarction was induced in rat hearts by occlusion left anterior descending artery (LAD). One week after LAD occlusion, rats were divided into three groups and subjected to transplantation ASCs or cell culture medium (CCM) remained untreated. During a 1-mo recovery period, magnetic resonance imaging showed that ASC-treated had significantly greater ventricular (LV)...
Complete and predicable regeneration of complex periodontal structures, which include cementum, ligament (PDL), alveolar bone, has been a great challenge for researchers. It is generally believed that human PDL from the root surface contains stem cells (r-PDLSCs), can enhance cementum/PDL-like tissues in vivo. In this work, was found to possess asymmetrically distributed observed by long-term bromodeoxyuridine (BrdU) labeling. Putative on bone (a-PDLSCs) were then isolated characterized....
Abstract An inflammatory microenvironment may cause organ degenerative diseases and malignant tumors. However, the precise mechanisms of inflammation-induced are not fully understood. Here, we show that proinflammatory cytokines interferon-γ (IFN-γ) tumor necrosis factor α (TNF-α) synergistically impair self-renewal differentiation mesenchymal stem cells (MSCs) via nuclear κB (NFκB)-mediated activation mothers against decapentaplegic homolog 7 (SMAD7) in ovariectomized (OVX) mice. More...
Abstract Background Mesenchymal stem/stromal cells (MSCs) are multipotent with a promising application potential in regenerative medicine and immunomodulation. However, MSCs cultured vitro exhibit functional heterogeneity. The underlying molecular mechanisms that define MSC heterogeneity remain unclear. Methods We investigated the gene expression profile via single-cell RNA sequencing (scRNA-seq) of human primary Wharton’s jelly-derived (WJMSCs) from three donors. also isolated CD142 + −...
This study was designed to examine the effects of bone marrow stromal cells (MSCs) cultured in vitro with or without neurotrophic factors transplanted into adult male Wistar rats after traumatic brain injury (TBI). MSCs harvested from donor were either culture medium containing brain-derived factor (BDNF) and nerve growth (NGF) same media these factors. Control experimental animals then traumatized by a controlled cortical impact. One day impact, placebo washed (1 × 106) NGF BDNF adjacent...
Background Erythropoietin (EPO), a hematopoietic cytokine, enhances neurogenesis and angiogenesis during stroke recovery. In the present study, we examined effect of EPO on oligodendrogenesis in rat model embolic focal cerebral ischemia. Methodology Principal Findings Recombinant human (rhEPO) at dose 5,000 U/kg (n = 18) or saline was intraperitoneally administered daily for 7 days starting 24 h after onset. Treatment with rhEPO augmented actively proliferating oligodendrocyte progenitor...
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stroma which can provide a potential therapy for diabetes mellitus. But the mechanism is still controversial. Also, status of BM-MSCs under hyperglycemia not known. In present study, we investigated in experimental-diabetic rat and demonstrated rescue experimental by diabetic MSCs transplantation.BM-MSCs were cultured multiple-differentiation was identified through induction into osteoblasts. passage 3 used following...
In this study, a cell-free tissue-engineered tracheal substitute was developed, which is based on 3D-printed polycaprolactone scaffold coated with gelatin-methacryloyl (GelMA) hydrogel, transforming growth factor-β1 (TGF-β) and stromal cell-derived factor-1α (SDF-1) sequentially embedded, to facilitate cell recruitment differentiation toward chondrocyte-phenotype. TGF-β loaded onto polydopamine particles, then encapsulated into the GelMA together SDF-1, called G/S/P@T, used coat PCL form...
Myocardial infarction (MI) is one of the leading causes death worldwide and Mesenchymal Stem Cells (MSCs) transplantation has been considered a promising therapy. Recently, it was reported that therapeutic effectiveness MSCs dependent on age donor, yet underlying mechanism not thoroughly investigated. This study designed to investigate whether this impaired potency caused by an increased susceptivity from old donors reactive oxygen species (ROS). The were isolated subcutaneous inguinal...