Acute myocardial infarction (AMI) accounts for the majority of deaths caused by coronary artery disease (CAD). Early warning AMI, especially patients with stable (sCAD), is urgently needed. Our previous study showed that alterations in gut microbiota were correlated CAD severity. Herein, we tried to discover accurate and convenient biomarkers AMI combination fecal/blood/urinary metabolomics. We recruited 190 volunteers including 93 sCAD patients, 49 48 subjects normal (NCA), measured their...

The hamster has been previously found to be a suitable model study the changes associated with diet-induced hyperlipidemia in humans. Traditionally, studies of utilize serum- or plasma-based biochemical assays and histopathological evaluation. However, unbiased metabonomic technologies have potential identify novel biomarkers disease. Thus, obtain better understanding progression discover biomarkers, we used proton nuclear magnetic resonance spectroscopy (1H-NMR)-based metabonomics approach...

Thirteen new thiodiketopiperazines, epicoccin I (1), ent-epicoccin G (2), and epicoccins J−T (3−13), together with six known diketopiperazines (14−19), have been isolated from the endophytic fungus Epicoccum nigrum. The structures of 1, 2, 10 were confirmed by X-ray crystallography, absolute configurations 4, 6, 8 assigned using Moshers' method. Compounds 12, 17 showed potent activities in vitro against release β-glucuronidase rat polymorphonuclear leukocytes induced platelet-activating...

A norditerpene glucopyranoside with a novel carbon skeleton (1), eight new aromatic glycosides (2−9), and 25 known have been isolated from H2O-soluble portion of an ethanolic extract the stem bark Fraxinus sieboldiana. Their structures were determined by spectroscopic chemical methods. Based on analysis NMR data threo- erythro-arylglycerols in different solvents, application ΔδC8-C7 values to distinguish threo-arylglycerol erythro-arylglycerol isomers was proposed. In vitro assays, compound...

A quantitative nuclear magnetic resonance (qNMR) method to measure the content of Orlistat in tablets was studied and found be efficient, accurate, reliable, simple. In this paper, phloroglucinolanhydrous dimethylsulfoxide-d₆ (DMSO-d₆) served as internal standard solvent, respectively. The qNMR methodology, including linearity, range, limit detection (LOD) quantification (LOQ), stability, precision, accuracy, validated seriatim, results were very favorable. determination three batches almost...

Three novel sesquinlignans, tatanans A (1), B (2), and C (3), have been isolated from the rhizomes of Acorus tatarinowii Schott. Their structures were established by spectroscopic techniques single-crystal X-ray analysis. Tatanans A−C potently increase GK enzymatic activity with EC1.5 values in range 0.16−1.85 μM. The potent unique structural features make them promising leads for therapeutic development antihyperglycemic drugs.

Five new stilbene dimers, gnetuhainins A−E (1−5), were isolated together with resveratrol trans-dehydrodimer (6), resveratrol, oxyresveratrol, and (−)-ε-viniferin from the lianas of Gnetum hainanense. Their structures stereochemistry determined on basis their chemical spectral data. Compounds 1−5 are dimers formed by a unit an oxyresveratrol belong to type oligostilbenes polymerized two different units.

Bioassay-guided fractionation of 95% EtOH extract from the roots Dipsacus asper lead to isolation some phenolic acids (caffeic acid, 2,6-dihydroxycinnamic vanillic 2′-O-caffeoyl-D-glucopyranoside ester, and caffeoylquinic acid) as major active components, five new iridoid glucoside dimers (1—5) one monomer (6), other known glycosides loganin, cantleyoside, triplostoside A, lisianthioside, 6′-O-β-D-apiofuranosyl sweroside, well triterpenoids oleanic acid akebiasaponin D. The structures...

Atherosclerosis (AS) is a progressive disease that contributes to cardiovascular and shows complex etiology, including genetic environmental factors. To understand systemic metabolic changes identify potential biomarkers correlated with the occurrence perpetuation of diet-induced AS, we applied 1H NMR-based metabolomics detect time-related profiles plasma, urine, liver extracts from male hamsters fed high fat cholesterol (HFHC) diet. Conventional biochemical assays histopathological...

In this study, 1H NMR-based metabonomics was applied to evaluate the beneficial effects of cordycepin (3′-deoxyadenosine), a natural monomer compound, on endogenous metabolic profiles liver and plasma from hyperlipidemic Syrian golden hamsters. Hyperlipidemia successfully established in hamsters fed by high-fat diet for 2 weeks. The were treated with an oral administration simvastatin (2 mg kg− 1) or (140 consecutive 4 intact tissues using NMR spectroscopy. results showed higher contents...

Nuclear magnetic resonance (NMR)-based metabolomics can be used directly to identify a variety of metabolites in biological fluids and tissues. Metabolite analysis is an important part life science research. However, the identification some using NMR spectroscopy remains big challenge owing low abundance or signal overlap. It develop method measure these compounds accurately. Two-dimensional spectroscopy, metabolite prediction software packages, spike-in experiments with authentic standards...

Two unusual glycosidic triterpene alkaloids, machilaminosides A (1) and B (2), have been isolated from the stem barks of Machilus yaoshansis. Their structures were elucidated by detailed spectroscopic analysis. possible biogenetic origin 1 2 mediated coupling 2-O-β-d-glucopyranosyl-cucurbitacin I, respectively, with urea adenosine was postulated. showed nonselective cytotoxic activities against several human cancer cell lines as well TNF-α secretion inhibitory activities.

Four new iridoid glycosides, 10-O-trans-p-coumaroylscandoside (1), 10-O-cis-p-coumaroylscandoside (2), 10-O-trans-p-coumaroyldesacetyl asperulosidic acid (3), and 10-O-cis-p-coumaroyldesacetyl (4), two grayanane diterpenoids, 14β-O-(2S,3S-nilyl)-2α,3β,5β,6β,16α-pentahydroxygrayanane (5) 14β-O-(2S,3S-nilyl)-2α,3β,5β,6β,16α-pentahydroxygrayan-10(20)-ene (6), have been isolated from Craibiodendron henryi. The structures of these compounds were determined by chemical spectroscopic methods...

Fractionation of the ethanolic extract stem bark Morus macroura resulted in isolation four new Diels-Alder type adducts, named guangsangons K--N (1, 2, 5, 6), together with two known compounds, mulberrofuran G (3) and K (4). Their structures were determined on basis spectroscopic analyses chemical methods. Furthermore, by means (1)H-NMR variable temperature experiments Cotton curves circular dichroism (CD) spectra, stereochemistry compounds was elucidated. The isolated showed good activity...

Pharmaceutical research of hyperlipidemia has been commonly pursued using traditional approaches. However, unbiased metabonomics attempts to explore the metabolic signature in a high-throughput manner understand pathophysiology disease process.As new way, we performed (1)H NMR-based evaluate beneficial effects 2',3',5'-tri-acetyl-N(6)- (3-hydroxylaniline) adenosine (WS070117) on plasma and liver from hyperlipidemic Syrian golden hamsters. Both profiles provided clearer distinction between...

Hydroxysafflor yellow A (HSYA) is the main active component of Chinese herb Carthamus tinctorius L.. Purified HSYA used as a neuroprotective agent to prevent cerebral ischemia. Injectable safflor (50 mg, containing 35 mg HSYA) widely treat patients with ischemic cardiocerebrovascular disease. However, it unknown how exerts protective effect on ischemia at molecular level. systematical integrated study, including histopathological examination, neurological evaluation, blood-brain barrier...

A simple, rapid, accurate, and selective quantitative nuclear magnetic resonance method for the determination of tadalafil in bulk drugs its tablets was established evaluated. Spectra were obtained dimethylsulfoxide-d6 using 2,4-dinitrotoluene as internal standard. In this study, method's linearity, range, limit quantification, stability, precision, accuracy validated. The results consistent with those from high-performance liquid chromatography analysis. Thus, proposed is a useful practical...

From the lianas of Gnetum montanum Markgr. f. megalocarpum Markgr., four new stilbenoids, gnetumontanins A - D (1 4) were obtained together with 16 known compounds. Gnetumontanin (1) is first oligostilbene dimerized from two oxyresveratrol units, gnetumontanin B (2) a stilbene trimer oligomerized and one resveratrol C (3) stilbenoid novel skeleton having δ-lactone moiety (4) glucoside 3. Their structures determined by means spectroscopic evidence, especially 2D NMR techniques. showed potent...

Three new anthraquinones, 1-hydroxy-2,3-dimethoxy-7-methyl-9,10-anthraquinone, 1,3-dihydroxy-5,6-dimethoxy-2-methyl-9,10-anthraquinone, and 3-hydroxy-1,5,6-trimethoxy-2-methyl-9,10-anthraquinone, along with five known anthraquinones were isolated from the roots of Prismatomeris tetrandra. Their structures determined on basis spectroscopic data.

Two new iridoid glucoside tetramers, dipsanosides A (1) and B (2), the first-reported tetramers with four glucosides, were isolated from Dipsacus asper. Their structures determined by analysis of 1D 2D NMR data as well comparison model compounds. cytotoxicities tested, but neither them showed obvious activity.