A5029577068- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Analytical Chemistry and Chromatography
- Ginseng Biological Effects and Applications
- Cancer, Lipids, and Metabolism
- Natural product bioactivities and synthesis
- Phytochemicals and Antioxidant Activities
- Traditional Chinese Medicine Analysis
- Analytical Methods in Pharmaceuticals
- Microfluidic and Capillary Electrophoresis Applications
- Image and Object Detection Techniques
- Advanced Nanomaterials in Catalysis
- Antibiotics Pharmacokinetics and Efficacy
- Immune cells in cancer
- Industrial Vision Systems and Defect Detection
- Analytical chemistry methods development
- Electrochemical sensors and biosensors
- Advancements in Transdermal Drug Delivery
- Advanced Photocatalysis Techniques
- Marine Biology and Environmental Chemistry
- Cholesterol and Lipid Metabolism
- Ocular Surface and Contact Lens
- Chromatography in Natural Products
Shandong Academy of Sciences
2019-2025
Qilu University of Technology
2019-2025
Peking University
2024-2025
Hunan Normal University
2025
Southwest Jiaotong University
2024
Anhui Provincial Hospital
2024
Anhui Medical University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2008-2023
Second Hospital of Shandong University
2017
China Pharmaceutical University
1989-2015
Characterization of tumor metabolism with spatial information contributes to our understanding complex cancer metabolic reprogramming, facilitating the discovery potential vulnerabilities that might be targeted for therapy. However, given variability and flexibility tumors, it is still challenging characterize global alterations in heterogeneous cancer. Here, we propose a spatially resolved metabolomics approach discover tumor-associated metabolites enzymes directly their native state. A...
Abstract Mapping tumor metabolic remodeling and their spatial crosstalk with surrounding non-tumor cells can fundamentally improve our understanding of biology, facilitates the designing advanced therapeutic strategies. Here, we present an integration mass spectrometry imaging-based metabolomics lipidomics microarray-based transcriptomics to hierarchically visualize intratumor heterogeneity cell interactions in same gastric cancer sample. Tumor-associated reprogramming is imaged at...
Histological examination with a deep link between functional metabolites and tissue structure biofunctions will provide important in situ biochemical information, then essentially reveal what has happened at the molecular level. However, due to complexity heterogeneity of samples large number metabolites, it is still challenge globally map diverse especially for those low-abundance ones. Here, sensitive air flow-assisted desorption electrospray ionization mass spectrometry imaging method...
New insights into tumor-associated metabolic reprogramming have provided novel vulnerabilities that can be targeted for cancer therapy.Here, we propose a mass spectrometry imaging (MSI)-based metabolomic strategy to visualize the spatially resolved of carnitine metabolism in heterogeneous breast cancer.Methods: A wide coverage MSI method was developed investigate spatial alternations carnitines tissues xenograft mouse models and human samples.Spatial expression key enzymes are closely...
Mass spectrometry imaging (MSI) has become a powerful tool to probe molecule events in biological tissue. However, it is widely held viewpoint that one of the biggest challenges an easy-to-use data processing software for discovering underlying information from complicated and huge MSI dataset. Here, user-friendly full-featured including three subsystems, Solution, Visualization Intelligence, named MassImager, developed focusing on interactive visualization, in-situ biomarker discovery...
Carboxyl-containing metabolites (CCMs) play indispensable roles in cell energy metabolism and cell-cell signaling. Profiling tissue CCMs with spatial signatures is significant for the understanding of molecular histology may provide new clues to uncover complex metabolic reprogramming organisms response external or internal stimuli. Here, we develop a sensitive on-tissue derivatization method, coupled matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI),...
It is highly challenging to quantitatively map multiple analytes in biotissues without specific chemical labeling. Quantitative mass spectrometry imaging (QMSI) has this potential but still poses technical issues for its variant ionization efficiency across a complicated, heterogeneous biomatrices. Herein, self-developed air-flow-assisted desorption electrospray (AFADESI) introduced present proof of concept method, virtual calibration (VC) QMSI. This method screens and utilizes analyte...
Panax notoginseng is a highly valued medicinal herb used widely in China and many Asian countries. Its root rhizome have long been for the treatment of cardiovascular hematological diseases. Imaging spatial distributions dynamics metabolites heterogeneous plant tissues significant characterizing metabolic networks notoginseng, this will also provide informative approach to understand complex molecular changes processing notoginseng.Here, high-sensitive MALDI-MS imaging method was developed...
A photoelectrochemical enzymatic biofuel cell consisting of a (methyl violet/Au nanoparticles) 25 /fluorine-doped tin oxide (FTO) photoanode without conventional semiconductor(s) and bilirubin oxidase/CuCo 2 O 4 /FTO bio-photocathode is developed.
The multi-omics approach combining MSI-based metabolomics and N -glycomics visualized spatial distributions of metabolites -glycans in heterogeneous colorectal cancer tissues.
Abstract The water oxidation reaction (WOR) with sluggish kinetics usually fails to adequately furnish protons oxygen reduction (ORR), which ultimately constrains the overall efficiency of photocatalytic synthesis H 2 O , particularly in absence sacrificial agents. Herein, a class hydroxyl groups (─OH) functionalized covalent organic framework (COF) is reported as proton reservoir compensate ORR for greatly improving photosynthesis. It has been demonstrated that incorporation ─OH into...
Salvia miltiorrhiza Bge (SMB) has long been used in traditional Chinese medicine to treat cardiovascular and cerebrovascular diseases. Growing clinical usage led a huge demand for artificial planting of SMB. Thus, continuous cropping SMB is an important challenge that needs be addressed. Continuous can alter the metabolic profile plants, resulting poor growth low yield. In this study, we tried image spatial location variation endogenous metabolites continuously cropped using matrix-assisted...
The pathological diagnosis of benign and malignant follicular thyroid tumors remains a major challenge using the current histopathological technique. To improve accuracy, spatially resolved metabolomics analysis based on air flow-assisted desorption electrospray ionization mass spectrometry imaging (AFADESI-MSI) technique was used to establish molecular diagnostic strategy for discriminating four types tumor. Without any specific labels, numerous metabolite features with their spatial...
The metabolic cross-talk between tumor and immune cells plays key roles in cell function checkpoint blockade therapy. However, the characterization of immunometabolism its spatiotemporal alterations during response a complex microenvironment is challenging. Here, 3D tumor-immune coculture spheroid model was developed to mimic interactions, combined with mass spectrometry imaging-based spatially resolved metabolomics visualize immunometabolic response. inhibition T simulated by coculturing...
Ion suppression from the tissue matrix has a severe effect on mass spectrometry imaging (MSI) of drugs. This problem hinders further applications MSI in preclinical drug research and development. In this study, an situ hydrogel conditioning method was developed to enhance sensitivity air-flow-assisted desorption electrospray ionization (AFADESI)-MSI. Instead traditional washing or digestion treatment solvent, used solid phase "wash" sections. It demonstrated that improved signal by as much...