A5084814704- Chromatography in Natural Products
- Natural product bioactivities and synthesis
- Analytical Chemistry and Chromatography
- Fungal Biology and Applications
- Traditional Chinese Medicine Analysis
- Phytochemistry and Biological Activities
- Food Quality and Safety Studies
- Neurological Disease Mechanisms and Treatments
- Pharmacological Effects of Natural Compounds
- Metabolomics and Mass Spectrometry Studies
- Ginkgo biloba and Cashew Applications
- Protein Hydrolysis and Bioactive Peptides
- Chemical synthesis and alkaloids
- Advanced Chemical Sensor Technologies
- Berberine and alkaloids research
- Phytochemical compounds biological activities
- Aquatic life and conservation
- Mass Spectrometry Techniques and Applications
- Bioactive Compounds and Antitumor Agents
- Essential Oils and Antimicrobial Activity
- Analytical chemistry methods development
- Graphene research and applications
- Herbal Medicine Research Studies
- Seaweed-derived Bioactive Compounds
- Free Radicals and Antioxidants
Qilu University of Technology
2018-2024
Shandong Academy of Sciences
2015-2024
Wuhan University
2022
Renmin Hospital of Wuhan University
2022
Committee on Publication Ethics
2022
Creative Commons
2022
Analysis and Testing Centre
2016
National Center for Toxicological Research
2015
First Institute of Oceanography
2008-2014
Chinese Academy of Sciences
2014
CE-based techniques with DAD and detection ESI-TOF-MS have been developed for the analysis of seven protoberberine alkaloids one aporphinoid alkaloid in Huanglian (Rhizoma coptidis), a well-known traditional Chinese herbal medicine. One aqueous BGE nonaqueous were CE-DAD CE-MS analyses, CE-ESI-TOF-MS conditions including nebulizer gas pressure, sheath-liquid composition, its flow rate, etc. optimized. Eight main R. coptidis could be separated baseline resolution by these two different BGEs,...
A method combining hydrophilic interaction chromatography (HILIC) and electrospray ionization mass spectrometry (ESI-MS) was developed for the characterization determination of natural Cordyceps. Separation achieved on a Waters Xbridge Amide column with gradient elution. Identification 15 target nucleosides nucleobases based retention time, UV spectra measurements protonated molecules ([M+H]⁺) main fragment ions (ESI-TOF/MS). Eight non-target compounds were tentatively identified by...
In this work, flavonoid fraction from the leaves of Crataegus pinnatifida was separated into its seven main constituents using a combination HSCCC coupled with pre-HPLC. first step, total extract subjected to two-solvent system chloroform/methanol/water/n-butanol (4:3:2:1.5, v/v), yielding four pure compounds, namely (–)-epicatechin (1), quercetin-3-O-(2,6-di-α-l-rhamnopyranosyl)-β-d-galactopyranoside (2), 4′′-O-glucosylvitexin (3) and 2′′-O-rhamnosylvitexin (4) as well mixture three further...
We put forward an efficient strategy based on bioassay guidance for the rapid screening, identification, and purification of neuraminidase inhibitors from traditional Chinese medicines, apply to discovery anti‐influenza components Lithospermiun erythrorhizon Sieb.et Zucc. Ultrafiltration with high‐performance liquid chromatography electrospray ionization time‐of‐flight mass spectrometry was employed screening preliminarily identification Zicao. Semipreparative used separation target...
Goji berries are now becoming increasingly popular in the human diet due to their potential health benefits. Unscrupulous traders deliberately mislabel with certain origins gain illegal profits, which seriously affected consumers' In this study, an online ultra-performance liquid chromatography-2,2-diphenyl-1 -picrylhydrazyl-photodiode array detector-electrospray ionization-quadrupole time of flight mass was developed for rapid screening and identification antioxidants from berry; then,...
Headspace gas chromatography-ion mobility spectrometric (HS-GC-IMS) fingerprint of volatile organic compounds (VOCs) in Lonicerae japonicae flos (LJF, Jinyinhua Chinese) was developed. This method, combined with chemometrics analysis, explored the identification authentic LJF. Seventy VOCs were identified from LJF, including aldehydes, ketones, esters, etc. The developed volatile-compound based on HS-GC-IMS coupled PCA analysis can successfully discriminate LJF its adulterant: japonicae(LJ,...
Pyrrolizidine alkaloids (PAs) are hepatotoxic, genotoxic, and carcinogenic in experimental animals. Because of their widespread distribution the world, PA-containing plants probably most common poisonous affecting livestock, wildlife, humans. Upon metabolism, PAs generate reactive dehydro-PAs other pyrrolic metabolites that lead to toxicity. Dehydro-PAs known react with glutathione (GSH) form 7-GSH-(+/-)-6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine (7-GS-DHP) vivo vitro 7,9-diGS-DHP...
An improved method based on HPLC-TOF/MS was developed to catalog the antioxidants in five species of Chaenomeles (Mugua). Forty-four fractions from Mugua extracts show appreciable levels antioxidative activity scavenging stable free-radical 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals. Twelve major antioxidant's chemical structures are identified. Antioxidant activities differ between species, but intra-species level antioxidants, regardless their ripeness, similar. C. sinensis has...
Abstract A new method has been developed based on ultrasound‐assisted extraction (UAE) followed by hydrophilic interaction chromatography (HILIC) and electrospray ionization time‐of‐flight mass spectrometry (ESI‐TOF/MS) for the simultaneous determination of 16 nucleosides nucleobases in medicinal extracts various marine organisms. The separation was achieved a Venusil HILIC column (250×4.6 mm id, 5 μm) gradient elution using solution acetonitrile buffer (0.20% formic acid 20 mmol/L ammonium...
In this paper, a method based on HPLC coupled with ESI-TOF/MS and on-line to stable free radical scavenging detection system has been developed rapidly screen identify the major antioxidants in water extract of Hippocampus japonicus Kaup, kind marine medicinal organism. The from H. Kaup revealed presence three compounds, which were simultaneously identified as hypoxanthine, phenylalanine tryptophane by high-resolution TOF-MS. To best our knowledge, it is original demonstrate rapid successful...
Compared with the conventional HSCCC, PZRCCC is an efficient and rapid method for separation of quaternary ammonium alkaloids because high concentration fractions large sample loading capacity.
Abstract Introduction Lycium barbarum is an edible fruit widely used in herbal medicines and as a functional food. Polysaccharide one of the most important active ingredients. Only L. grown Ningxia region China are officially recognised suitable for use traditional Chinese medicine, but systematic comparison polysaccharide between other growing regions has been rarely reported. Objective To compare difference from different China. Methods A chemical fingerprint hydrolysates was established...