Panax quinquefolius L. (PQ), a commonly used traditional Chinese medicine and food, is usually processed into various products, including white PQ, red PQ (two- or three-time steamed PQ), black (nine-time PQ). Previous studies demonstrated that volatile components (VOCs) were the important active substances of which had antibacterial, antiviral, anti-leukemia activities. However, most research focused on ginsenosides, few PQ. This study gas chromatography-ion mobility spectrometry to analyze...

Deep eutectic solvents (DESs) play important roles in the extraction of active constituents traditional Chinese medicine. Ultrasound-assisted DES has been used to extract flavonoids from

This study demonstrates the feasibility of producing three polysaccharides (neutral LJP-1, acidic LJP-2 and LJP-3) with significant <em>in vitro</em> vivo</em> anti-inflammatory activities from flowers <em>Lonicera japonica</em>. The differed in chemical composition, Mw distribution, glycosidic linkage pattern, functional groups morphology. They exhibited excellent protective effects (in a dose-dependent manner) lipopolysaccharide-injured RAW264.7 macrophages CuSO₄-damaged...

ABSTRACT Gancao Fuzi decoction (GCFZT) is a traditional Chinese formula, which has been commonly used in clinical practice to treat inflammatory diseases. However, the active substance of GCFZT treatment inflammation not fully clarified. In this study, we orthogonal experiments design different formulations, resulting total 16 formulations. Subsequently, UPLC‐Q‐TOF‐MS/MS was analyze chemical composition and anti‐inflammatory activity differences these formulations were evaluated through an...

American ginseng (AG) has long been used as an ingredient in the food and pharmaceutical industries because of its nutritional economic value. AG is rich nutrients, quality greatly affected by how it processed. However, there a relative paucity research on comprehensive evaluation different processing techniques AG. This study evaluated differences formation properties low-temperature softened, blanched, steamed followed hot air drying, vacuum freeze-dried (LTS-HAD, BL-HAD, ST-HAD, VFD,...

Burdock roots are healthy dietary supplements and a kind of famous traditional Chinese medicine, which contains large amounts caffeoylquinic acid derivatives. However, little research has been reported on the preparative separation these compounds from burdock roots. In present study, combinative method HSCCC semi-preparative HPLC was developed for derivatives The ethyl acetate extract first fractionated by MCI macroporous resin chromatography give three fractions (Fr. 1-3) elution 40%...

Diaphragma juglandis fructus contains various bioactive constituents. Fourteen compounds were isolated from by preparative high performance liquid chromatography (pre-HPLC) and high-speed counter-current (HSCCC). Their structures identified nuclear magnetic resonance (NMR) electrospray ionization mass spectrometry (ESI-MS). Compounds (+)-dehydrovomifoliol (12), (6R,9R)-9-hydroxymegastigman-4-en-3-one (13) (6R,9S)-9-hydroxymegastigman-4-en-3-one (14) are found Juglans regia L. for the first...

Non-alcoholic fatty liver disease (NAFLD) is the main cause of chronic disease, and its pathological development closely related to gut-liver axis. The intestinal barrier, an important component axis, can prevent gut microbes endotoxins from entering liver. Intestinal barrier function impaired in patients with NAFLD. Baicalein, which flavonoid Scutellariae Radix, improve However, whether baicalein alleviates NAFLD by ameliorating dysfunction remains unclear. In this study, a...

Gastrodia elata Blume (G. elata) is a prominent traditional herb and its dry tuber officially listed in the Chinese Pharmacopoeia. To ensure quality of dried G. elata, establishment nondestructive convenient method to monitor drying process necessary. In this study, low-field nuclear magnetic resonance (LF-NMR) imaging (MRI) was introduced elata. Three water states (bound, immobilized, free) samples were investigated through multiexponential fitting inversion NMR data. The variation...

Small-molecule (m/z<500) natural products have rich biological activity and significant application value thus need to be effectively detected. Surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) has become a powerful detection tool for small-molecule analysis. However, more efficient substrates developed improve the efficiency of SALDI MS. Thus, platinum nanoparticle-decorated Ti3C2 MXene (Pt@MXene) was synthesized in this study as an ideal substrate MS positive ion...

Triterpene acids were extracted from the epidermis of Poria cocos (Schw.) Wolf. These found to inhibit growth lung cancer cells in vitro and vivo. An efficient method for preparative separation antitumor triterpene was established that involves combination pH-zone-refining counter-current chromatography conventional high-speed chromatography. We used concentrate using a two-phase solvent system composed petroleum ether/ethyl acetate/methanol/water (3:7:5:5, v/v/v/v), trifluoroacetic acid (10...

Myricetin is a bioactive compound in many edible plants with anti-inflammatory and anticarcinogenic activity. The current study aimed to determine the protective effects mechanism of myricetin against ulcerative colitis (UC).Myricetin was orally administered at doses 40 80 mg/kg C57BL/6 mice UC induced using dextran sulfate sodium. disease-associated index colon length were determined end experiment, proportion Treg, Th1 Th17 analysed by cytometry, cytokines detected ELISA.Myricetin (80...

The dried roots of Scutellaria baicalensis are important traditional Chinese medicine used to treat liver and lung inflammation. An anomalous structure, hollowed root, was discovered in perennial cultivated baicalensis. presence the hollow may change contents bioactive metabolites, such as baicalein, other 4'-hydroxyflavones roots, but relationship between root metabolite is poorly understood. In this study, we identified anatomical structure detected differentially accumulating flavonoid...

Maren Runchang pill (MRRCP) is a Chinese patent medicine used to treat constipation in clinics. It has multi-component and multi-target characteristics, there an urgent need screen markers ensure its quality. The aim of this study was quality MRRCP based on "differential compounds-bioactivity" strategy using machine learning network pharmacology the effectiveness stability MRRCP. In study, UPLC-Q-TOF-MS/MS identify chemical compounds algorithms were applied differential compounds. further...