Neurons depend on proper localization of neurotrophic receptors in their distal processes for function. The Trk family neurotrophin controls neuronal survival, differentiation, and remodeling are well known to function as retrograde signal carriers transported from the axon toward cell body. However, mechanism driving anterograde trafficking into is not established. We used microfluidic compartmental devices inducible secretion assay systematically investigate routes TrkB receptor along rat...

ABSTRACT In neurons, the microtubule (MT) cytoskeleton forms basis for long-distance protein transport from cell body into and out of dendrites axons. To maintain neuronal polarity, axon initial segment (AIS) serves as a physical barrier, separating somatodendritic compartment acting filter axonal cargo. Selective trafficking is further instructed by enrichment MT post-translational modifications, which affect dynamics activity motor proteins. Here, we compared two knockout mouse lines...

The metabolic cross-talk between tumor and immune cells plays key roles in cell function checkpoint blockade therapy. However, the characterization of immunometabolism its spatiotemporal alterations during response a complex microenvironment is challenging. Here, 3D tumor-immune coculture spheroid model was developed to mimic interactions, combined with mass spectrometry imaging-based spatially resolved metabolomics visualize immunometabolic response. inhibition T simulated by coculturing...

Members of the SH3- and ankyrin repeat (SHANK) protein family are considered as master scaffolds postsynaptic density glutamatergic synapses. Several missense mutations within canonical SHANK3 isoform have been proposed causative for development autism spectrum disorders (ASDs). However, there is a surprising paucity data linking mutation-induced changes in structure dynamics to occurrence ASD-related synaptic phenotypes. In this proof-of-principle study, we focus on two ASD-associated point...

Article22 December 2021Open Access Source DataTransparent process Caldendrin and myosin V regulate synaptic spine apparatus localization via ER stabilization in dendritic spines Anja Konietzny RG Optobiology, Institute of Biology, Humboldt Universität zu Berlin, Germany Guest Group Neuronal Protein Transport, Center for Molecular Neurobiology, ZMNH, University Medical Hamburg-Eppendorf, Hamburg, Search more papers by this author Jasper Grendel Alan Kadek orcid.org/0000-0002-7953-5870 Leibniz...

In mammalian axon-carrying–dendrite (AcD) neurons, the axon emanates from a basal dendrite, instead of soma, to create privileged route for action potential generation at initial segment (AIS). However, it is unclear how such unusual morphology established and whether structure function AIS in AcD neurons are preserved. By using dissociated hippocampal cultures as model, we show that development can occur prior synaptogenesis independently vivo environment. A single precursor neurite first...

The home treatment of elderly patients with chronic heart failure (CHF) is often accompanied by malnutrition, which increases the risk re-hospitalisation and affects prognosis. Therefore, how to effectively improve nutritional self-management a current focus medical research. This study aims test effect home-based intervention method on improving status CHF.

Eclipta prostrata L. has been used in traditional medicine and known for its liver-protective properties centuries. Wedelolactone (WEL) demethylwedelolactone (DWEL) are the major coumarins found L.. However, comprehensive characterization of these two compounds on non-alcoholic fatty liver disease (NAFLD) still remains to be explored. Utilizing a well-established zebrafish model thioacetamide (TAA)-induced injury, present study sought investigate impacts mechanisms WEL DWEL NAFLD through...

Arsenic (As) is a ubiquitous environmental and industrial toxin with known correlates of oxidative stress cognitive deficits in the brain. Nuclear factor erythroid 2-related 2 (Nrf2) transcriptional that represents central cellular antioxidant defense mechanism transcribes many genes. Peroxisome proliferator-activated receptor-gamma (PPARγ) well-known nuclear receptor to regulate lipid metabolism tissues, it has been also associated control stress, neuronal death, neurogenesis...

(Developmental Cell 56, 494–508.e1–e7; February 22, 2021) It has come to our attention that in the above article, Figure S4D, showing PSM values of proteins detected Bio-Kif-td pull-down, second column, “Bio-Kif16-td+BirA+GFP,” is missing, resulting a shift data table and absence last column for “Bio-Kif1a-td+BirA +GFP.” The corresponding raw excel file Table S2, from which S4D was created, no errors. authors regret these errors, have been corrected online version article. corrections do not...

Reprogrammed metabolic network is a key hallmark of cancer. Profiling cancer alterations with spatial signatures not only provides clues for understanding biochemical heterogeneity, but also helps to decipher the possible roles reprogramming in development.Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) technique was used characterize expressions fatty acids breast tissues. Specific immunofluorescence staining further carried out investigate acid...

The evolution of starch synthase (SS) has been extensively analysed in the model plant rice, but there have few detailed studies directed towards exploring intron splicing variant effect on divergence SS gene. We report here an example that alternative a GC-AG induced new isoform SSII-1 expressed Oryza sativa japonica cultivar Zhonghua-15. In addition, GT-AG flanking was simultaneously spliced at incorrect position, inducing another fragment gained mature RNA. Moreover, when from SSII-3...

Summary In mammalian axon-carrying-dendrite (AcD) neurons, the axon emanates from a basal dendrite, instead of soma, to create privileged route for action potential generation at initial segment (AIS). However, it is unclear how such unusual morphology established and whether structure function AIS in AcD neurons preserved. Here, we show that follow an intrinsically encoded developmental program where single precursor neurite first gives rise then AcD. The possesses similar cytoskeletal...

Abstract Members of the SH3- and ankyrin-rich repeat (SHANK) protein family are considered as master scaffolds post-synaptic density glutamatergic synapses. Several missense mutations within canonical SHANK3 isoform have been proposed causative for development autism spectrum disorders (ASDs). However, there is a surprising paucity data linking mutation-induced changes in structure dynamics to occurrence ASD-related synaptic phenotypes. In this work, we focus on two ASD-associated point...

In the light of problems traditional personal credit evaluation index system and model, such as less indexes small coverage, an based on a big data platform is established with model basis XGBoost (Extreme Gradient Boosting) proposed. By introducing Bayesian Optimization Algorithm(BOA), WOE IV indicators to optimize we try improve classification performance generalization ability finally verify availability through interpretability analysis by using SHAP(Shapley Additive expressions). Then...

Abstract In neurons, the microtubule (MT) cytoskeleton forms basis for long-distance protein transport from cell body into and out of dendrites axon. To maintain neuronal polarity, axon initial segment (AIS) serves as a physical barrier, separating somatodendritic compartment acting filter axonal cargo. Selective trafficking is further instructed by enrichment MT post-translational modifications, which affect dynamics activity motor proteins. Here, we compared two knockout mouse lines...

The development of the economy and society is inseparable from convenient transportation. In recent decades, road transportation industry has developed rapidly, number tunnels also increased accordingly. As one important components tunnels, load lighting system cannot be underestimated. that adopts maximum illuminance principle problems such as excessive energy consumption high operating costs. To address this, it necessary to promote implementation tunnel intelligent systems (TILS). This...

Traditional frequency droop control (TFDC) strategies can utilize deviation and DC voltage to provide support in source converter-based multi-terminal direct current transmission (VSC-MTDC) systems. However, the coefficient is fixed real-time operation status of grid converter station capacity are often neglected when unbalanced power should be allocated. This lead overload significant fluctuation weak grid. Therefore, a novel strategy proposed this paper. Firstly, allocation relationship...

This paper proposes an anti-disturbance controller for bridge cranes with two-stage swing effects (DPOC). Firstly, the transition process is arranged by tracking differential (TD), which not only enhances robustness of controller, but also solves contradiction between overshoot and rapidity. In addition, expansion state observer (ESO) designed to observe uncertain external disturbance system, then compensated nonlinear control law (NLSEF), so as realize positioning anti-swing double pendulum...