A5100640267- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Monoclonal and Polyclonal Antibodies Research
- Mast cells and histamine
- Identification and Quantification in Food
- Contact Dermatitis and Allergies
- Transgenic Plants and Applications
- Asthma and respiratory diseases
- Retinoids in leukemia and cellular processes
- Animal Genetics and Reproduction
- Arsenic contamination and mitigation
- Genomics, phytochemicals, and oxidative stress
- Pineapple and bromelain studies
- Drug-Induced Adverse Reactions
- Probiotics and Fermented Foods
- Biopolymer Synthesis and Applications
Gannan Medical University
2018-2024
Nanchang University
2014-2023
State Key Laboratory of Food Science and Technology
2014-2023
Abstract BACKGROUND Bovine β ‐lactoglobulin (BLG) is the major allergen in cows' milk, and specific epitope plays a key role food allergy. Developing method specifically bind to IgE necessary for testing BLG its allergenic residues. RESULTS The monoclonal antibody (1G9) linear was identified as high affinity specificity. Based on 1G9, sensitive fluorescent sandwich enzyme‐linked immunosorbent assay (sELISA) successfully developed using catalase‐mediated fluorescence quenching of thiolated...
Cow's milk allergy is one of the most frequent and severe IgE-induced food allergies for children, demanding sensitive analytical methods, β-lactoglobulin (BLG) can be as an important biomarker detection protein. In this study, a highly sandwich enzyme-linked immunosorbent assay (sELISA) based on specific polyclonal antibody against human IgE linear epitopes BLG anti-BLG antibody-platinum nanoparticles probe was described BLG. This sELISA exhibited ultrawide range 0.49–1.6 × 104 ng/mL,...
Abstract BACKGROUND β ‐Lactoglobulin is recognised as one of major allergens in milk and its epitopes include linear conformational contributed to allergy. RESULTS In our work, two types have been identified. Linear identified by using SPOT™ peptide arrays approach three common sequences AA77 –82 ( KIPAVF ), AA126 –131 PEVDNE ) AA142 –147 ALPMHI were obtained reacting with specific sera from rabbits. At the same time, mimotopes screened panning a phage display library corresponding...
Bovine β-lactoglobulin (BLG) is a common allergen found in milk, and the immunoglobulin E (IgE) epitope plays crucial role cow milk allergy. Therefore, targeting IgE could be useful accurately detecting BLG assessing its allergenicity. However, producing an epitope-specific antibody (IgE-EsAb) through traditional methods requires complex time-consuming procedures. Here, IgE-EsAb was purified from rabbit anti-BLG sera by immunomagnetic beads one step. Then, sandwich ELISA (sELISA) based on...
Background Peanut is an important source of dietary protein for human beings, but it also recognized as one the eight major food allergens. Binding IgE antibodies to specific epitopes in peanut allergens plays roles initiating peanut-allergic reactions, and Ara h 2 widely considered most potent allergen best predictor allergy. Therefore, can serve useful biomarkers prediction IgE-binding variations foods. This study aimed develop validate epitope-specific (IgE-EsAbs)-based sandwich ELISA...
Arsenic (As) is a ubiquitous environmental and industrial toxin with known correlates of oxidative stress cognitive deficits in the brain. Nuclear factor erythroid 2-related 2 (Nrf2) transcriptional that represents central cellular antioxidant defense mechanism transcribes many genes. Peroxisome proliferator-activated receptor-gamma (PPARγ) well-known nuclear receptor to regulate lipid metabolism tissues, it has been also associated control stress, neuronal death, neurogenesis...
Bovine αS1-casein (αS1-CN) is the main allergen in cow's milk, and immunoglobulin E (IgE) epitopes are material basis to trigger allergic reactions. Thus, IgE could be as key targets for precise αS1-CN detection allergenicity assessment. Here, we designed a tandem containing nine linear of (tαS1-CN), expressed recombinant tαS1-CN Escherichia coli, prepared rabbit polyclonal antibody against (pAb-tαS1-CN). The obtained had purity 94.43% retained antigenicity potential epitopes. pAb-tαS1-CN...
Bovine β-lactoglobulin (BLG) is one of the major allergens in cow's milk. Epitopes play a key role food allergy, while most antibodies developed do not specifically recognize IgE-binding epitopes. Here, tandem derived from human IgE linear epitopes BLG (tBLG) was designed according to principles epitope-based vaccine methodology, expressed Escherichia coli, and polyclonal antibody (pAb-tBLG) developed. The recombinant tBLG protein preserved antigenicity potential allergenicity pAb-tBLG...
Although αs1-casein is the main allergens in cow’s milk and make severe effects on multiple systems even cause anaphylactic shock, reliable quantification method of this allergen still limited until now. We therefore developed an immunomagnetic beads-based immunoassay (IMBs-ELISA) for quantitative detection based epitopes. The monoclonal antibody (mAb) agaist epitope bound to magnetic beads was used as capture probe a horseradish peroxidase (HRP) labelled polyclonal (pAb) employed detector...