A5103050591- Metabolomics and Mass Spectrometry Studies
- Traditional Chinese Medicine Analysis
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Advanced Chemical Sensor Technologies
- Natural product bioactivities and synthesis
- Tea Polyphenols and Effects
- Food Quality and Safety Studies
- Adipose Tissue and Metabolism
- Ginseng Biological Effects and Applications
- Metabolism and Genetic Disorders
- Plant biochemistry and biosynthesis
- Zebrafish Biomedical Research Applications
- Health, Environment, Cognitive Aging
- Gene expression and cancer classification
- Phytochemicals and Antioxidant Activities
- Physiological and biochemical adaptations
- Allelopathy and phytotoxic interactions
- Chromatography in Natural Products
- Biochemical Analysis and Sensing Techniques
- Protein Hydrolysis and Bioactive Peptides
- Ziziphus Jujuba Studies and Applications
- Phytochemistry and Biological Activities
- Rheumatoid Arthritis Research and Therapies
- Flavonoids in Medical Research
State Ethnic Affairs Commission
2021-2025
Minzu University of China
2018-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2017-2021
Peking Union Medical College Hospital
2017
Xinjiang Institute of Materia Medica
2017
Detailed knowledge on tissue-specific metabolic reprogramming in diabetic nephropathy (DN) is vital for more accurate understanding the molecular pathological signature and developing novel therapeutic strategies. In present study, a spatial-resolved metabolomics approach based air flow-assisted desorption electrospray ionization (AFADESI) matrix-assisted laser (MALDI) integrated mass spectrometry imaging (MSI) was proposed to investigate alterations kidneys of high-fat diet-fed...
Diabetes cardiomyopathy (DCM) is a metabolic disease and leading cause of heart failure among people with diabetes. Mass spectrometry imaging (MSI) versatile technique capable combining the molecular specificity mass spatial information imaging. In this study, we used MSI to visualize metabolites in rat high resolution sensitivity. We optimized air flow-assisted desorption electrospray ionization (AFADESI)-MSI platform detect wide range metabolites, then matrix-assisted laser (MALDI)-MSI for...
Quantitative metabolomics approaches can significantly improve the repeatability and reliability of investigations but face critical technical challenges, owing to vast number unknown endogenous metabolites lack authentic standards. The present study contributes development a novel method known as "data-independent targeted quantitative metabolomics" (DITQM), which was used investigate label-free multiple in biofluid samples. This approach initially involved acquisition MS/MS data for all...
Spatially resolved metabolic profiling of brain is vital for elucidating tissue-specific molecular histology and pathology underlying diabetic encephalopathy (DE). In this study, a spatially metabolomic method based on air-flow-assisted desorption electrospray ionization–mass spectrometry imaging (AFADESI–MSI) was developed investigating the region-specific disturbances in DE model rats induced by high-fat diet combination with streptozotocin administration. A total 19 discriminating...
Evaluating toxicity and decoding the underlying mechanisms of active compounds are crucial for drug development. In this study, we present an innovative, integrated approach that combines air flow-assisted desorption electrospray ionization mass spectrometry imaging (AFADESI-MSI), time-of-flight secondary ion (ToF-SIMS), spatial metabolomics to comprehensively investigate nephrotoxicity nitidine chloride (NC), a promising anti-tumor candidate. Our quantitive AFADESI-MSI analysis unveiled...
Polygala tenuifolia, commonly known as Yuanzhi (YZ) in Chinese, has been shown to possess anti-insomnia properties. However, the material basis and mechanism underlying its sedative-hypnotic effects remain unclear. Herein, we investigated active components neurochemical of YZ extracts using liquid chromatography tandem mass spectrometry (LC-MS/MS)-based pharmacometabolomics imaging (MSI)-based spatial resolved metabolomics. According results, 17 prototypes out 101 ingredients extract were...
In metabolomics research, the use of different blood collection methods may influence endogenous metabolites.Ultra HPLC coupled with MS/MS was applied together multivariate statistics to investigate differences in serum and plasma samples handled by anticoagulants. A total 135 known representative metabolites were assessed for comprehensive evaluation effects anticoagulants.Exogenous factors, including separation gel ingredients from tubes anticoagulants, affected mass spectrometer...
Fatty aldehydes (FALs) are involved in various biological processes, and their abnormal metabolism is related to the occurrence development of neurological diseases. Because low ionization efficiency, methods for situ detection mass spectrometry imaging (MSI) analysis FALs remain underreported. On-tissue chemical tagging hardly ionizable target analytes with easily ionized moieties can improve efficiency sensitivity MSI experiments. In this study, an on-tissue...
Zebrafish (Danio rerio) represent an effective model biological material for human disease research, even personalized precision medicine. Thus, it is necessary to fully characterize their molecular information in order obtain a global metabolic profile. Here, spatially resolved metabolomics method whole-body zebrafish analysis was established based on air-flow-assisted desorption electrospray ionization-mass spectrometry imaging (AFADESI-MSI) system. Using the optimized experimental...
Understanding the renal region-specific metabolic alteration in different animal models of diabetic nephropathy (DN) is critical for uncovering underlying mechanisms and developing effective treatments. In present study, spatially resolved metabolomics based on air flow-assisted desorption electrospray ionization mass spectrometry imaging (AFADESI-MSI) was used to compare local changes kidneys HFD/STZ-induced rats db/db mice. As a result, total 67 59 discriminating metabolites were...
Casper, a type of transparent mutant-line zebrafish, was generated to overcome the opaque trunk an adult zebrafish for tumor modeling realize real-time visualization transplanted cells in vivo. However, molecular information at metabolic level has not received much attention. Herein, spatially resolved metabolomics method based on airflow-assisted desorption electrospray ionization–mass spectrometry imaging (AFADESI-MSI) system whole-body used investigate small molecules and distribution...
A metabolomic study of three glioma cell lines with different stemness were conducted. The specific metabolite signatures associated SLC self-renewal and differentiation characterized.
Wuyi rock tea is one of the best oolong in China, and Yanyun key quality component tea. It has been well known that closely influenced by production region, but its underlying mechanism still unclear. In this study, 112 plantations were randomly selected from three main regions mountain area, including Zhengyan, Banyan Zhou region. total, 1120 fresh leaf samples 224 soil collected analyzed. The phenotype was identified based on metabolomic analysis through ultra-high performance liquid...