The present study was conducted to evaluate the chemical composition and antioxidant activity of essential oils ethanol extracts Ocimum basilicum L. obtained from Assiut, Minia BeniSuef Egypt. major constituents sweet basil three locations were linalool, estragole, methyl cinnamate, bicyclosesquiphellandrene, eucalyptol, α-bergamotene, eugenol, γ-cadinene germacrene D by method GC–MS. relative concentration these compounds varies. contained appreciable levels total phenolic contents...

Detailed knowledge on tissue-specific metabolic reprogramming in diabetic nephropathy (DN) is vital for more accurate understanding the molecular pathological signature and developing novel therapeutic strategies. In present study, a spatial-resolved metabolomics approach based air flow-assisted desorption electrospray ionization (AFADESI) matrix-assisted laser (MALDI) integrated mass spectrometry imaging (MSI) was proposed to investigate alterations kidneys of high-fat diet-fed...

Diabetes cardiomyopathy (DCM) is a metabolic disease and leading cause of heart failure among people with diabetes. Mass spectrometry imaging (MSI) versatile technique capable combining the molecular specificity mass spatial information imaging. In this study, we used MSI to visualize metabolites in rat high resolution sensitivity. We optimized air flow-assisted desorption electrospray ionization (AFADESI)-MSI platform detect wide range metabolites, then matrix-assisted laser (MALDI)-MSI for...

In the Chinese traditional medicine, plant of Agastache rugosa (Fisch. & C.A. Mey.) Kuntze (A. rugosa) has been used to treat nausea, vomiting and dispel damp. However, currently, few reports about chemical constituents, especially non-volatile components A. are available.Through separation with various column chromatographies elucidate constituents rugosa, biological activities major were investigated. The extracts main evaluated for their anticoagulant effects by assaying activated partial...

Human health is threatened by foodborne illness and Staphylococcus aureus a common pathogenic bacteria. It can cause food poisoning when we are infected, therefore, it necessary to detect The virulence genes detection methods of S. summarized from literatures. Traditional simple but need bacteria enrichment prolong time. Immunological technology has high specificity, false positive results easy occur in its detection. In recent years, molecular biology have developed rapidly, various PCR...

Spatially resolved metabolic profiling of brain is vital for elucidating tissue-specific molecular histology and pathology underlying diabetic encephalopathy (DE). In this study, a spatially metabolomic method based on air-flow-assisted desorption electrospray ionization–mass spectrometry imaging (AFADESI–MSI) was developed investigating the region-specific disturbances in DE model rats induced by high-fat diet combination with streptozotocin administration. A total 19 discriminating...

Nigella sativa is a valuable herb for its functional compositions in both food and medication. N. seeds can enhance immunity, anti-inflammation analgesia hypoglycemia, but most of the related researches are to volatile oil extracts, activity mechanism compounds not clear. In this study, Ethyl-α-D-galactopyranoside (EG), Methyl-α-D-glucoside (MG), 3-O-[β-D-xylopyranose-(1 → 3)-α-L-rhamnose-(1 2)-α-L-arabinose]-28-O-[α-L-rhamnose-(1 4)-β-D-glucopyranose-L-(1 6)-β-D-glucopyranose]-hederagenin...

Ionic liquid 1-butyl-3-methylimidazolium bromide-methanol-based ultrasonic-assisted extraction (ILUAE) was used to extract tilianin and acacetin from the aerial parts of Agastache rugose (A. rugose), simultaneously determined by reversed phase high performance chromatographic (RP-HPLC) method with ultraviolet detection (RP-HPLC-UV). An InertSustain RP-C18 column mobile consisting methanol 0.2% acetic acid as gradient elution at wavelength 332 nm. The flow rate 0.8 mL/min, temperature 30 °C....

Antioxidant and α-glucosidase inhibitiory active compounds of Cercis chinensis flowers were investigated with bio-assay guiding method. Ethyl acetate fraction (CLEa) n-butanol (CLBu) exhibited antioxidant activity in vitro, the corresponding fractions, EaFr.3, EaFr.5 BuFr.1, higher than those other fractions. Eight compounds, ethyl gallate (1), stearic acid (2), docosanoic (3), 5α-stigmast-9(11)-en-3β-ol (4), kaempferol-3-O-α-rhamnopyranoside (5), vanillic (6), fisetin (7), β-sitosterol (8),...

The compounds of Rubus spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, their antithrombotic effects mechanism investigated with the acute blood stasis rat model. RSB extract was evaluated activated partial thromboplastin time (APTT), thrombin (TT), prothrombin (PT), fibrinogen (FIB) assays in vitro. Results indicated that exhibited anticoagulant activity. In addition to 1 6, other also activity Therefore, vivo antithrombosis measuring whole viscosity (WBV),...

Five polysaccharides, namely ADPs-1a, ADPs-1b, ADPs-2, ADPs-3a and ADPs-3b, were extracted from Angelicae dahuricae Radix, purified, identified by high performance gel permeation chromatography (HPSEC), gas (GC), Fourier transform infrared (FT-IR) spectrometer nuclear magnetic resonance spectra (NMR), including the determination of procoagulant activity in vitro. The average molecular weight (Mw) polysaccharides was 153,800, 8312, 111,700, 3766 96,680 g/mol, respectively. Coagulation assays...

The blackberry seed was typically removed as a byproduct and waste from fruits for juices. Developing value-added utilization of berry seeds will significantly expand the market products well improve benefit to producers. However, research on is limited. objective this paper antithrombotic mechanism polysaccharides in seeds. Polysaccharides were extracted, purified identified by high-performance gel permeation chromatography (HPSEC), gas (GC), fourier transform infrared (FT-IR) spectrometer...

Aim: Alizarin (AZ), that can be isolated from Rubia cordifolia, has biological activities such as antioxidation and anti-inflammatory. This study aimed to investigate the effect of AZ on glucose lipid metabolism disorders in alloxan-induced diabetic mice also explored insulin resistance 3T3-L1 adipocytes. Results: The research showed could decrease fasting postprandial blood glucose, TG, TC MDA, it increase liver glycogen levels SOD activity mice. significantly improve uptake adipocytes...

Cichorium endivia. L, consumed either cooked or eaten raw in salads, is a popular kind of vegetable cultivated all around the World. Its components have been widely used folk medicine anti-inflammatory therapy. However, anti-cancer activity has never reported. In this study, (3S)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (1), an amino isolated from C. was found for first time to show cytotoxic colorectal cancer cell line HCT-8. Compound 1 at concentrations 0.5–4 μM induced apoptosis...

Trelagliptin inhibits the enzyme dipeptidyl-4 (DPP-4) to treat type 2 diabetes and it may possess potential improve insulin resistance. However, molecular mechanism is not known. In this study, effect of trelagliptin succinate in improving resistance was investigated. The differentiation system 3T3-L1 mouse preadipocytes used determine content adipokines GLUT4 outer membrane. expression AKT, P-AKT, IRS-1 P-IRS-1 differentiated adipocytes determined by western blotting. Our results...

Three lignans, phillyrin, forsythia ester A, and rosin-β-D-furan glucose, were isolated from Forsythia suspensa which is a famous Traditional Chinese Medicine used for clearing heat detoxifying, reducing swelling dispersing knot, wind heat. In this study, the effects of glucose on insulin resistance 3T3-L1 adipocytes investigated by method oxidase-peroxidase (GOD-POD) mechanism was assayed western blot. The results indicated that could improve uptake in under (IR). Among them, phillyrin...

Fatty aldehydes (FALs) are involved in various biological processes, and their abnormal metabolism is related to the occurrence development of neurological diseases. Because low ionization efficiency, methods for situ detection mass spectrometry imaging (MSI) analysis FALs remain underreported. On-tissue chemical tagging hardly ionizable target analytes with easily ionized moieties can improve efficiency sensitivity MSI experiments. In this study, an on-tissue...

is an important plant pathogenic fungus of many crops. Our previous study identified the