A5065400012- Ubiquitin and proteasome pathways
- Renal cell carcinoma treatment
- RNA and protein synthesis mechanisms
- Sunflower and Safflower Cultivation
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- interferon and immune responses
- Peptidase Inhibition and Analysis
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Genomics and Phylogenetic Studies
- Mass Spectrometry Techniques and Applications
- Iron Metabolism and Disorders
- Heavy Metal Exposure and Toxicity
- Histone Deacetylase Inhibitors Research
- Island Studies and Pacific Affairs
- Hops Chemistry and Applications
- Caveolin-1 and cellular processes
- Medicinal Plants and Bioactive Compounds
- Chemical Reactions and Isotopes
- Extracellular vesicles in disease
Shanghai Customs College
2024
National Engineering Research Center for Nanotechnology
2023
State Key Laboratory of Biotherapy
2016-2022
Sichuan University
2010-2022
Chengdu Center for Disease Control and Prevention
2022
Northwest A&F University
2018-2019
State Key Laboratory of Molecular Oncology
2018
Nanjing Medical University
2018
West China Hospital of Sichuan University
2018
Chinese Academy of Medical Sciences & Peking Union Medical College
2018
Abstract Objectives The correlations between long non‐coding RNAs (lncRNAs) and diverse mammal diseases have been clarified by many researches, but the cognition about bovine mastitis‐related lncRNAs remains limited. This study aimed to investigate potential role of lncRNA X‐inactive specific transcript (XIST) in inflammatory response mammary epithelial cells. Materials methods Two alveolar cell‐T (MAC‐T) models were established infecting cells with Escherichia coli (E. coli) Staphylococcus...
// Bing Chen 1, * , Xiao Zeng Yu He 1 Xixi Wang Ziwei Liang Jingjing Liu Peng Zhang 2 Hongxia Zhu 3 Ningzhi Xu Shufang State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P.R. Department Urinary Surgery, Sichuan, P. R. Cell Molecular Biology & Oncology, Institute Chinese Academy Medical Sciences, Beijing, 100034, These authors contributed equaly to this work Correspondence to:...
Phosphoglycerate kinase 1 (PGK1) has complicated and multiple functions in cancer occurrence, tumor progression drug resistance. Sorafenib is the first-line treatment targeted for patients with kidney renal clear cell carcinoma (KIRC) as a tyrosine inhibitor, but sorafenib resistance extremely common to retard therapy efficiency. So far, it unclear whether how PGK1 involved pathogenesis of KIRC. Herein, molecular mechanisms PGK1-mediated KIRC have been explored by comprehensively integrative...
Hepatocellular carcinoma (HCC) is a major cause of cancer-related death worldwide. Up to date, HCC pathogenesis has not been fully understood. The aim the present study was identify crucial genes and pathways associated with by bioinformatics methods. differentially expressed (DEGs) between 14 tissues corresponding non-cancerous were identified using limma package. Gene Ontology (GO) KEGG pathway enrichment analysis DEGs performed clusterProfiler protein-protein interaction (PPI) network...
The SUMO-activating enzyme SAE1 is indispensable for protein SUMOylation. A dysregulation of expression involves in progression several human cancers. However, its biological roles glioma are unclear by now. differential proteome between tissues and para-cancerous brain were identified LC-MS/MS. was further assessed immunohistochemistry. patient overall survival versus level evaluated Kaplan–Meier method. cell growth migration under overexpression or inhibition the CCK8, transwell assay...
We previously reported the inhibitory effects of microRNA-26a (miR-26a) on conversion pyruvate to acetyl coenzyme A in glucose metabolism by directly targeting dehydrogenase protein X component colorectal cancer (CRC) cells (Chen B et al., BMC Cancer 2014). Here, using microRNA situ hybridization, we confirmed that miR-26a levels were elevated 77 human CRC tissue samples and further investigated key miR-26a-mediated metabolic regulation elements signaling pathways through quantitative...
In tumor microenvironment, interactions among multiple cell types are critical for cancer progression. To understand the molecular mechanisms of these complex interplays, secreted protein analysis between malignant cells and surrounding nonmalignant stroma is a good viewpoint to investigate cell-cell interactions. Here, we developed two stable isotope labeling amino acids in culture (SILAC)-based mass spectrometry (MS)/MS approaches termed spike-in SILAC triple-SILAC quantify changes...
Long noncoding RNAs (lncRNAs) play multiple key roles during inflammatory processes. In this study, a novel lncRNA identified by the high-throughput sequencing analysis was found significantly down-regulated in Escherichia coli-introduced cell model of bovine mastitis. Given that consists antisense leucine-rich repeat-containing protein 75A (LRRC75A), it named LRRC75A lncRNA1 (LRRC75A-AS1). The expression LRRC75A-AS1 mammary epithelial cells and tissues under condition. Knockout (KO)...
Progesterone receptor membrane component 1 (PGRMC1) is widely observed with an elevated level in multiple human cancers. However, the roles of PGRMC1 renal cancer are not clear and merit further study. In this report, we made a systematic, integrative biological assessment for cell carcinoma (RCC) by quantitative proteomic identification, immunohistochemical detection, its clinic pathologic significance analysis. We found that abundance increased 3.91-fold RCC tissues compared autologous...
Aberrant expression of Na+/K+-ATPase α1 subunit (ATP1A1) is widely observed in multiple types tumors, and its tissue-specific relates to cancer development. However, the functions molecular mechanisms renal cell carcinoma (RCC) are not fully understood. We investigated ATP1A1 changes possible roles RCC through a quantitative proteomic approach an integrative biochemical assessment. detected with LC–MS/MS, further validated immunohistochemical analyses 80 pairs tumor non-tumor tissues...
The protein ARHGDIA has been found to play distinct roles in cancer progression for several tumors. However, it remains elusive whether and how plays functions human glioma. In this study, we discovered that is much downregulated glioma; meanwhile, its expression negatively correlates with glioma malignancy positively relates prognosis of patients. It independent predictive value level overall survival Glioma patients ARHGDIA-positive have a longer time than ARHGDIA-negative Knockdown...
Context It has been found that hydroxysafflor-yellow A (HSYA) inhibits angiogenesis and the proliferation of abnormal human umbilical vein endothelial cells (HUVECs) in our previous study; however, mechanism is still unclear.Objective This study investigates mechanisms HSYA inhibiting HUVECs through detecting expression vascular growth factor (VEGF) its receptor (KDR), protein Ras-Raf-MEK-ERK-signalling pathway.Materials methods HepG2 cell cultural supernatant was used to culture make...
Summary Daptomycin (DAP) is a novel microbial lipopeptide antibiotic synthesized by the DAP biosynthetic gene cluster dpt of Streptomyces roseosporus (S. roseosporus) . DptP locates upstream and confers resistance to ambofaciens ambofaciens) So far, biological functions dptP for S. growth are still completely uncovered. We performed label‐free quantification proteomic dissections with loss‐ gain‐of‐function experiments decipher ‐involved functions. Deletion activated energy metabolism...
Abstract Background There have been reports of increased glutamate pyruvate transaminase 2 (GPT2) expression in certain cancers including breast cancer. Although the role GPT2 as a metabolic enzyme is well understood cancer progression, little known about other roles GPT2, especially exosomal GPT2. Methods BT549 and BT474 Cells were cultured their exosomes isolated by using ultracentrifugation. migrated through membrane stained with crystal violet, then observed microscope. Total RNA was...