A5085652628- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Nuclear Materials and Properties
- Advanced Proteomics Techniques and Applications
- Fusion materials and technologies
- High Temperature Alloys and Creep
- Cancer, Hypoxia, and Metabolism
- Advanced Photocatalysis Techniques
- Single-cell and spatial transcriptomics
- Metal-Organic Frameworks: Synthesis and Applications
- Immune Cell Function and Interaction
- Total Knee Arthroplasty Outcomes
- Orthopedic Infections and Treatments
- Nanoparticle-Based Drug Delivery
- Blood transfusion and management
- ZnO doping and properties
- Atmospheric chemistry and aerosols
- Autophagy in Disease and Therapy
- Corrosion Behavior and Inhibition
- Immune cells in cancer
- Intermetallics and Advanced Alloy Properties
- Luminescence and Fluorescent Materials
- Plant Pathogens and Fungal Diseases
- Microfluidic and Capillary Electrophoresis Applications
- Covalent Organic Framework Applications
National Institute of Metrology
2022-2025
Harbin Medical University
2025
Huaihua University
2024
Xi'an Jiaotong University
2021-2023
The Affiliated Yongchuan Hospital of Chongqing Medical University
2023
Chongqing Medical University
2023
China West Normal University
2022-2023
Tsinghua University
2019-2022
North University of China
2022
Division of Chemistry
2022
Comprehensive analysis of single-cell metabolites is critical since differences in cellular chemical compositions give rise to specialized biological functions. Herein, we propose a label-free mass cytometry by coupling flow ESI-MS (named CyESI-MS) for high-coverage and high-throughput detection metabolites. Cells suspension were isolated, online extracted sheath fluid, lysed during gas-assisted electrospray, followed real-time MS analysis. Hundreds metabolites, including nucleotides, amino...
It is generally believed that the electron-withdrawing cyano group in olefin linkage would inhibit stability and π-conjugation of covalent organic frameworks (COFs), which raises concerns about their optoelectronic properties. However, structure–activity relationship between structure linkages properties COFs still inconclusive. In this work, imine-, vinylene-, acrylonitrile-linked with identical triphenyltriazine building blocks were designed synthesized. Our work demonstrated construction...
Cancer stem cells (CSCs) are a small subset of malignant cells, possessing stemness, with strong tumorigenic capability, conferring resistance to therapy and leading the relapse nasopharyngeal carcinoma (NPC). Our previous study suggested that cyclooxygenase-2 (COX-2) would be novel target for CSCs-like side population (SP) in NPC. In present study, we further found COX-2 maintained stemness NPC by enhancing activity mitochondrial dynamin-related protein 1 (Drp1), fission mediator, studying...
While single-cell mass spectrometry can reveal cellular heterogeneity and the molecular mechanisms of intracellular biochemical reactions, its application is limited by insufficient detection sensitivity resulting from matrix interference sample dilution. Herein, we propose an intact living-cell electrolaunching ionization (ILCEI-MS) method. A capillary emitter with a narrow-bore, constant-inner-diameter ensures that entire living cell enters MS ion-transfer tube. Inlet improves utilization,...
Chemoresistance to triple-negative breast cancer (TNBC) is a critical issue in clinical practice. Lipid metabolism takes unique role cells; especially, unsaturated lipids involving cell membrane fluidity and peroxidation are highly remarked. At present, for the lack of high-resolution molecular recognition platform at single-cell level, it still hard systematically study chemoresistance heterogeneity based on lipid unsaturation proportion. By designing mass spectrometry workflow CyESI-MS, we...
Single-cell mass spectrometry (MS) is an essential technology for sensitive and multiplexed analysis of metabolites lipids cell phenotyping pathway studies. However, the structural elucidation from single cells remains a challenge, especially in high-throughput scenario. Technically, there contradiction between inadequate sample amount (
Label-free mass cytometry is utilized to study the dynamic metabolic change during apoptosis in HepG2 cells induced by NK92 MI at single-cell level. The heterogeneity of individual was revealed.
Changes of metabolite concentrations in single cells are significant for exploring the dynamic regulation important biological processes, such as cell development and differentiation. Accurate quantitation metabolites is essential analysis. In this work, we proposed a quantitative method single-cell by combining microwell array with droplet microextraction-mass spectrometry. The can confine both extraction solvent defined space, avoiding irregular spread trace internal standard solution...
Rapid and sensitive quantification of peptides plays an important role in clinical diagnosis. Fluorescence assay is one the most promising peptide detection tools, but it relies on intrinsic fluorescence or additional derivatization, resulting poor versatility. Covalent organic frameworks (COFs) have shown a good application prospect field detection, their scope limited to heavy metal ions some small polar molecules. Herein, we report COFs nanosheet for peptides. Fluorescent sp2...
Microplastics (MPs) are universally present in the ecosystem and pose great threats to environment living organisms. Research studies have shown that small MPs (<50 μm diameter) especially toxic account for more than half of all collected Atlantic Ocean. Nevertheless, current methods detection analysis incapable achieving rapid situ biota ultimately enable study their biological effects. In this work, we report a method allows identification spatial mapping directly from paramecia with high...
Background: Breast cancer has become the most common in women. Compare with other subtypes of breast cancer, triple-negative (TNBC) is more likely to relapse and metastasize. Highly effective therapeutic strategies are desperately needed be explored. In this study, a multifunctional nanoplatform expected mediate chemo-photothermal therapy, which can combine immunogenic cell death checkpoint blockade combat TNBC distant metastasis. Methods: Poly (lactic acid-glycolic acid)-Poly (ethylene...
Discrimination of cancer cells at the single-cell metabolic level is crucial for early diagnosis. However, some share similar information with normal cells, making them difficult to be distinguished using mass spectrometry. Herein, we propose a method by treating osteosarcoma and human osteoblasts mannose as stimulant, which greatly promotes discrimination level. The low PMI (mannose 6-phosphate isomerase) both cell lines compared reason abnormal pathway two treatment. We also found that...
FeCrAl alloys have been proposed as nuclear fuel cladding materials in hope of offering great potential accident tolerance for light water reactors. However, only a limited amount data has published on their creep properties. This work aims to provide an atomic-scale insight into the behavior and deformation mechanism nanocrystalline alloys, using molecular dynamics method. Both primary steady state creeps are observed all curves, tertiary occurs at stresses above 2.5 GPa time period present...
Discriminating various leukocyte subsets with specific functions is critical due to their important roles in the development of many diseases. Here, we proposed a general strategy unravel leukocytes heterogeneity and screen differentiated metabolites as biomarker candidates for subtypes using label-free mass cytometry (CyESI-MS) combined homemade data processing workflow. Taking leukemia cells an example, metabolic fingerprints single were obtained from 472 HL-60, 416 THP-1, 313 U937, 356...
To address the challenges in single-cell metabolomics (SCM) research, we have developed an open-source Python-based modular library, named SCMeTA, for SCM data processing. We designed standardized pipeline and inter-container communication format components to adapt diverse needs of studies. The validation was carried out on multiple experiment data. results demonstrated significant improvements batch effects, accuracy results, metabolic extraction rate, cell matching as well processing...
A schematic presentation of the mechanisms red-shifted and blue-shifted luminescence in (Ca<sub>1−x</sub>Ba<sub>x</sub>)<sub>5</sub>(PO<sub>4</sub>)<sub>3</sub>Cl:Eu<sup>2+</sup> (0 ≤ <italic>x</italic> 1) solid-solution phosphors.
Lymphocytes play crucial roles in the human immune system; however, detailed metabolite characteristics need to be further investigated. Herein, we propose a lymphocyte classification method based on profiling at single-cell level. The percentages of different types were calculated with low margin error, confirming that metabolites could serve as basis for classification. Furthermore, analyzed CD4/CD8 ratio peripheral blood verify feasibility this subtypes. proposed is expected potential...