Mass spectrometry-based lipidomics is the primary tool for structural analysis of lipids but effective localization carbon-carbon double bonds (C=C) in unsaturated to distinguish C=C location isomers remains challenging. Here, we develop a large-scale lipid platform by coupling online derivatization through Paternò-Büchi reaction with liquid chromatography-tandem mass spectrometry. This provides rich information on isomers, revealing locations more than 200 glycerophospholipids bovine liver...

Abstract A lipidome comprises thousands of lipid species, many which are isomers and isobars. Liquid chromatography-tandem mass spectrometry (LC-MS/MS), although widely used for lipidomic profiling, faces challenges in differentiating isomers. Herein, we address this issue by leveraging the orthogonal separation capabilities hydrophilic interaction liquid chromatography (HILIC) trapped ion mobility (TIMS). We further integrate isomer-resolved MS/MS methods onto HILIC-TIMS, enable pinpointing...

As a major class of mammalian lipids, phosphatidylcholines (PCs) often contain mixtures structural isomers, resulting from different lipogenesis pathways. Profiling PCs at the isomer level, however, remains challenging in lipidomic settings, especially for characterizing positions fatty acyls on glycerol backbone (sn-positions) and locations carbon-carbon double bonds (C[double bond, length as m-dash]Cs) unsaturated acyl chains. In this work, we have developed workflow profiling down to sn-...

Background: Associations between ulcerative colitis (UC) and ankylosing spondylitis (AS) have been reported in multiple studies, but the common etiologies of UC AS remain unknown. Thus, current study, we aimed to investigate shared genes relevant mechanisms AS. Methods: Using datasets for (GSE113079) (GSE1797879), initially identified differentially expressed (DEGs) through differential expression analysis. The DEGs from both were intersected identify DEGs, AS, which used receiver operating...

Abstract Mass spectrometry imaging (MSI) of lipids in biological tissues is useful for correlating molecular distribution with pathological results, which could provide information both research and disease diagnosis. It well understood that the lipidome not be clearly deciphered without tandem mass analysis, but this challenging to achieve MSI due limitation sample amount at each image spot. Here we develop a multiplexed MS 2 (MS I) method can images 10 lipid species or more sampling spot,...

The study of lipid metabolism relies on the characterization lipidome, which is quite complex due to structure variations species. New analytical tools have been developed recently for characterizing fine structures lipids, with C=C location identification as one major improvements. In this study, we studied reprograming by analyzing glycerol phospholipid compositions in breast cancer cell lines structural specification extended level. Inhibition desaturase, stearoyl-CoA desaturase 1,...

Abstract Glycosphingolipids (GSLs) are essential components of cell membranes, particularly enriched in the nervous system. Altered molecular distributions GSLs increasingly associated with human diseases, emphasizing significance lipidomic profiling. Traditional GSL analysis methods hampered by matrix effect from phospholipids and difficulty distinguishing structural isomers. Herein, we introduce a highly sensitive workflow that harnesses magnetic TiO 2 nanoparticle-based selective...

Plasmanyl and plasmenyl glycerophosphocholine are ether lipids featuring the 1-O-alkyl or 1-O-alk-1'-enyl linkage at sn-1 position of glycerol backbone, respectively. Aberrant levels glycerophosphocholines (ether PCs) have been correlated with cellular dysfunctions various human diseases. Profiling PCs accurate structural information is challenging because common presence isomeric isobaric species in a lipidome. The Paternò-Büchi (PB) reaction, double bond (C[double bond, length as m-dash]C)...

Phosphatidylethanolamines (PEs), ether-PEs, and phosphatidylserines (PSs) are glycerophospholipids harboring a primary amino group in their headgroups. They key components of mammalian cell membranes play pivotal roles signaling apoptosis. In this study, liquid chromatography–mass spectrometry (LC–MS) workflow for deep profiling PEs, PSs has been developed by integrating two orthogonal derivatizations: (1) derivatization the 4-trimethylammoniumbutyryl-N-hydroxysuccinimide (TMAB-NHS) enhanced...

The Paternò–Büchi (PB) reaction is a carbon–carbon double bond (C═C)-specific derivatization that can be used to pinpoint the location(s) of C═C(s) in unsaturated lipids and quantitate location isomers when coupled with tandem mass spectrometry (MS/MS). As data PB-MS/MS are increasingly generated, establishment corresponding analysis tool highly needed. Herein, LipidOA, machine-learning prior-knowledge-based tool, developed analyze generated by liquid chromatography–mass workflows. LipidOA...

Building energy consumption is the main urban component, which mainly serves people-centered work and living demands. Based on physical requirements of humans in buildings to determine comfortable body temperature each season, this paper establishes a sizing optimization model building-type integrated systems (IES) for sustainable development, where cooling heating loads required maintain indoor somatosensory comfort are calculated. Depending external price, internal power balance, other...

In-depth structural characterization of lipids provides a new means to investigate lipid metabolism. In this study, we have conducted deep profiling total fatty acids (FAs) from RAW 264.7 macrophages by utilizing charge-tagging Paternò-Büchi derivatization carbon-carbon double bond (C=C) and reversed-phase liquid chromatography-tandem mass spectrometry. A series FAs exhibiting unusual site(s) unsaturation was unearthed, with their identities being confirmed observing anticipated...

Abstract Mass spectrometry imaging (MSI) of lipids in biological tissues is useful for correlating molecular distribution with pathological results, which could provide information both research and disease diagnosis. It well understood that the lipidome not be clearly deciphered without tandem mass analysis, but this challenging to achieve MSI due limitation sample amount at each image spot. Here we develop a multiplexed MS 2 (MS I) method can images 10 lipid species or more sampling spot,...

To build a multi-energy cloud platform with the distributed generation, energy storage, micro-grid, flexible load, electric vehicle piles for high efficiency application is of great significance. In order to manage resources dispatching and trading in platform, this paper solves three problems. Firstly, present planning method. The modelling linear optimization algorithm prosumer’s self-balanced minimize cost quantity random bidding price are proposed. Secondly, key technologies realize...

Under the new electricity price policy mechanism, China's pumped storage units will enter spot market to participate in mediation and profit. At present, are strictly managed by dispatching orders. This paper establishes a profit model of under call on demand mode. By integrating their power curves real-time curves, operation simulation participating mode is carried out. The results indicate that normal trend curve, have certain profitability when operating according conventional pumping...

Condition based maintenance (CBM) is more targeted than regular and accident maintenance. Pumped-storage enterprises can effectively reduce the waste of manpower, material resources, financial resources in equipment by using condition This paper presents a system architecture design for pumped-storage units decision model considering probabilistic cost comparison, which verified an example. The results indicate that condition-based strategy after entering market influenced short-term...

With the increasing scale and complexity of power system operation, in order to further improve efficiency accuracy electromagnetic transient modeling large-scale systems, this paper proposes a set fast conversion methods from electromechanical simulation software BPA RTDS for electrical models. The key technologies model are introduced detail data parsing verification, pre-processing, sub-system division, automatic layout wiring, is applied study automation province Southern Power Grid....

With the increasing number and scale of VSCHVDC connected to power grid, impact VSC-HVDC on AC short-circuit current level needs be accurately calculated. Existing studies generally use simplified processing methods, it is difficult evaluate calculation error. This paper summarizes process mechanism contribution, establishes a typical electromagnetic transient model analyze influence current, analyzes error source existing method, puts forward an improved method considering both active...