A5051070739- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Lipid Membrane Structure and Behavior
- Lipid metabolism and biosynthesis
- Sphingolipid Metabolism and Signaling
- Chromatography in Natural Products
- IoT-based Smart Home Systems
- Computational Drug Discovery Methods
- Building Energy and Comfort Optimization
- Smart Grid Energy Management
- Advanced Multi-Objective Optimization Algorithms
- Genetics, Aging, and Longevity in Model Organisms
- Structural Health Monitoring Techniques
- Alcohol Consumption and Health Effects
- Endoplasmic Reticulum Stress and Disease
- Phytochemicals and Antioxidant Activities
- Fatty Acid Research and Health
- Alkaloids: synthesis and pharmacology
- Industrial Vision Systems and Defect Detection
Inner Mongolia University
2023-2025
Tsinghua University
2015-2022
As a major class of mammalian lipids, phosphatidylcholines (PCs) often contain mixtures structural isomers, resulting from different lipogenesis pathways. Profiling PCs at the isomer level, however, remains challenging in lipidomic settings, especially for characterizing positions fatty acyls on glycerol backbone (sn-positions) and locations carbon-carbon double bonds (C[double bond, length as m-dash]Cs) unsaturated acyl chains. In this work, we have developed workflow profiling down to sn-...
Sphingomyelins (SMs) are an essential class of lipids widely existing in different organisms. The sphingoid base and N-acyl chain two building blocks which can undergo types modifications during lipogenesis, including desaturation, hydroxylation, methyl branching. Current lipidomic analysis methods cannot provide detailed information on these structural motifs. Herein, we developed a tandem mass spectrometric method by harnessing radical-directed dissociation (RDD) from collision-induced...
Phosphatidylcholines (PCs) are the major structural components of plasma membrane mammalian cells, while lysophosphatidylcholines (LPCs) critical intermediates in lipid remodeling. Conventional tandem mass spectrometric (MSn) methods via collision-induced dissociation (CID) blind to intrachain modifications such as location carbon–carbon double bond (C═C) and methyl branching point. In this study, we demonstrate that almost complete information can be inferred from a single MS2 CID spectrum...
Lysophosphatidylcholine acyltransferase-1 (LPCAT1) plays a critical role in the remodeling of phosphatidylcholines (PCs) cellular lipidome. However, evidence is scarce regarding its sn-selectivity, viz. preference assembling acyl-Coenzyme A (CoA) at C1 or C2-hydroxyl on glycerol backbone because difficulty to quantify thus-formed PC sn-isomers. We have established multiplexed assay measure both sn- and acyl-chain selectivity LPCAT1 toward mixture acyl-CoAs by integrating isomer-resolving...
Chain modifications on fatty acyls, such as methyl branching, are important to modulate the biochemical and biophysical properties of lipids. The current lipid analysis workflows which mainly rely collisional-induced dissociation (CID) obtain structural information lipids often fail in locating chain modifications. Radical-directed (RDD) is a new type tandem mass spectrometry (MS/MS) method capable producing intrachain cleavages, thus allowing detailed characterization structures. In this...
Multiple isozymes are encoded in the Caenorhabditis elegans genome for various sphingolipid biosynthesis reactions, but contributions of individual characterized only part. We developed a simple effective reversed-phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) method that enables simultaneous identification and quantification ceramides (Cer), glucosylceramides (GlcCer), sphingomyelins (SM) from same MS run. Validating this profiling method, we show nearly all 47...
In this paper, three fatigue models are employed and proposed for modeling the life of different fiber-reinforced composite material systems. order to identify unknown parameters in these models, Genetic Algorithm (GA) is used estimating its values. This technique a stochastic process that leads straight S-N curves predicts trend experimental data without need any assumptions. The calculation results show especially nonlinear regression model, whose assigned by using GA all satisfied with...
Demand Side Energy Management System (DSEMS) manages energy demand by controlling end-use appliances in a refined, energy-saving, cost-efficient and user-friendly way. The DSEMS runs on an Android tablet computer, which serves as gateway to communicate with two types of controllers via ZigBee network. Smart sockets connected the network will monitor control plug-in loads IR remote controller (IRRC) for air-conditioner temperature setting. proposed system has been installed apartment over 24...
Koumiss is a fermented product with series of health benefit. Lipids, which are the essential nutrients, involved in diverse biological processes and associated physiological pathological states body. In previous studies, only fatty acid composition physico-chemical analysis koumiss were investigated. Little known concerning structures lipid species. this study, comprehensive profile home-made from Inner Mongolia region was achieved using high performance liquid chromatography-electrospray...
Abstract Lysophosphatidylcholine acyltransferase‐1 (LPCAT1) plays a critical role in the remodeling of phosphatidylcholines (PCs) cellular lipidome. However, evidence is scarce regarding its sn ‐selectivity, viz. preference assembling acyl‐Coenzyme A (CoA) at C1 or C2‐hydroxyl on glycerol backbone because difficulty to quantify thus‐formed PC ‐isomers. We have established multiplexed assay measure both ‐ and acyl‐chain selectivity LPCAT1 toward mixture acyl‐CoAs by integrating...