A5023466237- Pancreatic function and diabetes
- Liver physiology and pathology
- Mesenchymal stem cell research
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Machine Learning in Bioinformatics
- Electrospun Nanofibers in Biomedical Applications
- Genetics and Neurodevelopmental Disorders
- Nitric Oxide and Endothelin Effects
- Adipose Tissue and Metabolism
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Genomics and Phylogenetic Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Single-cell and spatial transcriptomics
- Lipid metabolism and biosynthesis
- Pregnancy and preeclampsia studies
- Hippo pathway signaling and YAP/TAZ
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Circular RNAs in diseases
- Organ Transplantation Techniques and Outcomes
- Genetics, Aging, and Longevity in Model Organisms
Tsinghua University
2022-2025
Beijing Tsinghua Chang Gung Hospital
2020-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2024
Gansu Provincial Hospital
2014-2024
Academy of Military Medical Sciences
2006-2021
Hebei Medical University
2021
Xingtai People's Hospital
2021
Qingdao University
2001-2020
Qingdao Municipal Hospital
2018-2020
Southern Medical University
2018
Abstract Multipotent stem/progenitors are present in peribiliary glands of extrahepatic biliary trees from humans all ages and high numbers hepato-pancreatic common duct, cystic hilum. They express endodermal transcription factors (e.g., Sox9, SOX17, FOXA2, PDX1, HES1, NGN3, PROX1) intranuclearly, stem/progenitor surface markers (EpCAM, NCAM, CD133, CXCR4), sometimes weakly adult liver, bile pancreatic genes (albumin, fibrosis transmembrane conductance regulator [CFTR], insulin)....
Current protocols for differentiation of stem cells make use multiple treatments soluble signals and/or matrix factors and result typically in partial to mature with under- or overexpression adult tissue-specific genes. We developed a strategy rapid efficient using substrata biomatrix scaffolds, extracts enriched extracellular matrix, associated growth cytokines, combination serum-free, hormonally defined medium (HDM) tailored the cell type interest. Biomatrix scaffolds were prepared by...
Peribiliary glands (PBGs) in bile duct walls, and pancreatic (PDGs) associated with ducts, humans of all ages, contain a continuous, ramifying network cells overlapping maturational lineages. We show that proximal (PBGs)-to-distal lineages start near the duodenum expressing markers pluripotency (NANOG, OCT4, SOX2), proliferation (Ki67), self-replication (SALL4), early hepato-pancreatic commitment (SOX9, SOX17, PDX1, LGR5), transitioning to PDG no expression or markers, maintenance genes...
In human aging, liver aging per se not only increases susceptibility to diseases but also vulnerability of other organs given its central role in regulating metabolism. Total function tends be well maintained the healthy elderly, so is generally difficult identify early. response this critical challenge, Aging Biomarker Consortium China has formulated an expert consensus on biomarkers by synthesizing latest scientific literature, comprising insights from both scientists and clinicians. This...
Abstract Islet cell replacement is considered as the optimal treatment for type I diabetes. However, availability of human pancreatic islets transplantation limited. Here, we show that bone marrow‐derived mesenchymal stem cells (hMSCs) could be induced to differentiate into functional insulin‐producing by introduction duodenal homeobox‐1 (PDX‐1). Recombinant adenoviral vector was used deliver PDX‐1 gene hMSCs. After being infected with Ad‐PDX‐1, hMSCs were successfully insulin‐secreting...
The high incidence rate of hepatocellular carcinoma (HCC) is mainly the result frequent metastasis and tumor recurrence. Unfortunately, underlying molecular mechanisms driving HCC are still not fully understood. It has been demonstrated that stroma cells contribute to primary growth metastasis. Within environment, activated hepatic stellate (HSCs) can release a number molecules enhance cancer cell proliferation invasiveness in paracrine manner. Here, for first time, we demonstrate epimorphin...
Axon degeneration is a common and often early feature of neurodegeneration that correlates with the clinical manifestations progression neurological disease. Nicotinamide mononucleotide adenylytransferase (NMNAT) neuroprotective factor delays axon following injury in models neurodegenerative diseases suggesting converging molecular pathway self-destruction. The underlying mechanisms have been under intense investigation recent reports suggest central role for axonal mitochondria both...
Preeclampsia, especially preterm preeclampsia and early-onset preeclampsia, is a life-threating pregnancy disorder, the heterogeneity complexity of make it difficult to predict risk develop treatments. Plasma cell-free RNA carries unique information from human tissue may be useful for noninvasive monitoring maternal, placental, fetal dynamics during pregnancy.
Non-alcoholic fatty liver disease is associated with hepatic microangiopathy and inflammation caused by type 2 diabetes mellitus. Oxidised LDL (oxLDL) involved in proinflammatory cytotoxic events various microcirculatory systems. The lectin-like oxLDL receptor 1 (LOX1) plays a crucial role oxLDL-induced pathological transformation. However, the underlying mechanism of oxLDL's effects on microcirculation disturbances remains unclear. In this study, we investigated LOX1 (OLR1) expression...
Abstract Retinal ganglion cells (RGCs), the sole output of retina, are a heterogeneous population neurons that project axons to visual targets in brain. Like most CNS neurons, RGCs considered incapable mounting long distance axon regeneration. Using immunolabeling-enabled 3D imaging solvent-cleared organs (iDISCO) transgenic mice, we tracked entire paths individual RGC and show adult highly capable spontaneous long-distance regeneration, even without any treatment. Our results Thy1-H-YFP...
Lenvatinib is the first-line treatment for hepatocellular carcinoma (HCC), most common type of primary liver cancer; however, some patients become refractory to lenvatinib. The underlying mechanism lenvatinib resistance (LR) in with advanced HCC remains unclear. We focused on exploring potential LR and novel treatments lenvatinib-resistant HCC. In particular, we established a Huh7 cell line performed vitro, bioinformatic, biochemical assays. Additionally, used Huh7-LR cell-derived xenograft...
The purpose of this study was to determine whether retrovirus-mediated transfer the murine macrophage inducible nitric oxide synthase (iNOS) gene can inhibit tumorigenicity and metastasis human renal cancer cells. Retroviral vectors encoding iNOS were constructed in pLXSN retroviral vector with under control a long terminal repeat promoter neomycin resistance an internal simian virus 40 promoter. Highly metastatic carcinoma SN12PM6 cells infected or retrovirus. Expression confirmed by...
Androgens provide survival signals to prostate epithelial cells, and androgen ablation induces apoptosis in the gland. However, molecular mechanisms of actions androgen-signaling pathway these processes are not fully understood. Here, we report that androgens induced expression cellular Fas/FasL-associated death domain protein-like inhibitory protein (c-FLIP) gene, which is a potent inhibitor Fas/FasL-mediated apoptosis. The receptor was recruited promoter c-FLIP gene presence androgens. We...
Gap junction-mediated cell-cell interactions are highly conserved and play essential roles in cell survival, proliferation, differentiation patterning. We report that Connexin 32 (Cx32)-mediated gap junctional intercellular communication (GJIC) is necessary for human embryonic stem cell-derived hepatocytes (hESC-Heps) during step-wise hepatic lineage restriction maturation. Vitamin K2, previously shown to promote Cx32 expression mature hepatocytes, up-regulated GJIC activation maturation,...