A5089759016- Ferroptosis and cancer prognosis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Renal cell carcinoma treatment
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- CNS Lymphoma Diagnosis and Treatment
- Sexual function and dysfunction studies
- Cholesterol and Lipid Metabolism
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Hormonal and reproductive studies
- Cancer Immunotherapy and Biomarkers
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Metabolomics and Mass Spectrometry Studies
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Sphingolipid Metabolism and Signaling
- Acute Myeloid Leukemia Research
- Peroxisome Proliferator-Activated Receptors
- Brucella: diagnosis, epidemiology, treatment
- Adipose Tissue and Metabolism
- Cancer, Lipids, and Metabolism
- Acute Lymphoblastic Leukemia research
Fudan University
2014-2025
Kettering University
2023-2025
Memorial Sloan Kettering Cancer Center
2021-2025
Changhai Hospital
2009-2024
Second Military Medical University
2009-2024
University of California, Los Angeles
2014-2024
Anhui Medical University
2024
BGI Group (China)
2015-2024
BGI Genomics
2024
Jiangnan University
2024
Mapping the mammalian brain The diverse physiology of is reflected in its complex organization at regional, cellular, and subcellular levels. Sjöstedt et al. combined data—both newly acquired from other large-scale mapping projects—from transcriptomics, single-cell genomics, situ hybridization, antibody-based protein profiling to map molecular profiles human, pig, mouse brain. analysis consistent with a conserved basic architecture during evolution, but it does show differences regional gene...
INTRODUCTION Although much effort has been devoted to studying yeast in the past few decades, our understanding of this model organism is still limited. Rapidly developing DNA synthesis techniques have made a “build-to-understand” approach feasible reengineer on genome scale. Here, we report completion 770-kilobase synthetic chromosome II (synII). SynII was characterized using extensive Trans-Omics tests. Despite considerable sequence alterations, synII virtually indistinguishable from wild...
Abstract Genetic causes of many familial arrhythmia syndromes remain elusive. In this study, whole‐exome sequencing ( WES ) was carried out on patients from three different families that presented with life‐threatening arrhythmias and high risk sudden cardiac death SCD ). Two French Canadian probands identical homozygous rare variant in TECRL gene (p.Arg196Gln), which encodes the trans ‐2,3‐enoyl‐CoA reductase‐like protein. Both had arrest, stress‐induced atrial ventricular tachycardia, QT...
Cancers arise through an evolutionary process in which cell populations are subjected to selection; however, date, the of bladder cancer, is one most common cancers world, remains unknown at a single-cell level. We carried out exome sequencing 66 individual tumor cells from muscle-invasive transitional carcinoma (TCC). Analyses somatic mutant allele frequency spectrum and clonal structure revealed that were derived single ancestral cell, but subsequent evolution occurred, leading two...
Objective To determine the effects of gastric bypass on myocardial lipid deposition and function plasma lipidome in women with obesity heart failure preserved ejection fraction (HFpEF). Methods A primary cohort ( N = 12) HFpEF underwent echocardiography magnetic resonance spectroscopy both before 3 months 6 after bariatric surgery. Plasma lipidomic analysis was performed surgery were confirmed a validation 22). Results After surgery‐induced weight loss, Minnesota Living Heart Failure...
Abstract Pigs are valuable large animal models for biomedical and genetic research, but insights into the tissue- cell-type-specific transcriptome heterogeneity remain limited. By leveraging single-cell RNA sequencing, we generate a multiple-organ transcriptomic map containing over 200,000 pig cells from 20 tissues/organs. We comprehensively characterize of in tissues identify 234 cell clusters, representing 58 major types. In-depth integrative analysis endothelial reveals high degree...
The extent of cell-to-cell variation in tumor mitochondrial DNA (mtDNA) copy number and genotype, the phenotypic evolutionary consequences such variation, are poorly characterized. Here we use amplification-free single-cell whole-genome sequencing (Direct Library Prep (DLP+)) to simultaneously assay mtDNA nuclear (nuDNA) 72,275 single cells derived from immortalized cell lines, patient-derived xenografts primary human tumors. Cells typically contained thousands copies, but was extensive...
Abstract Purpose: NOTCH signaling pathway is essential in T-cell development and NOTCH1 mutations are frequently present acute lymphoblastic leukemia (T-ALL). To gain insight into its clinical significance, mutation was investigated 77 patients with T-ALL. Experimental Design: Detection of done using reverse transcription-PCR amplification direct sequencing, thereby compared according to the clinical/biological data patients. Results: Thirty-two were identified 29 (with dual 3 cases),...
Metabolic variations occur during normal pregnancy to provide the growing fetus with a supply of nutrients required for its development and ensure health woman gestation. Mass spectrometry-based metabolomics was employed study metabolic phenotype in maternal plasma that are induced by each three trimesters. Nontargeted analysis showed significantly altered profile metabolites plasma. The levels six were found change throughout pregnancy, related pathway observed biopterin metabolism,...
The activation of liver X receptor (LXR) promotes cholesterol efflux and repression inflammatory genes with anti-atherogenic consequences. mechanisms underlying the repressive activity LXR are controversial have been attributed to or transrepression activator protein-1 (AP-1) activity. Here, we find that contributes repression, while direct functions also play a key role but independent AP-1. We use assay for transposase-accessible chromatin using sequencing (ATAC-seq) show reduces...
Rationale: Hepatocellular carcinoma (HCC) is a highly heterogeneous and malignant disease with the complex immune microenvironment, which ultimately influence clinic outcomes of patients.However, spatial expression patterns diverse cells among tumor microenvironment remain to be further deciphered.Methods: Spatial transcriptomics sequencing (ST) was implemented on two portions HCC specimens.Differentially expressed genes, cell cycle phases, epithelial-mesenchymal features, pseudo-time...
Preeclampsia, especially preterm preeclampsia and early-onset preeclampsia, is a life-threating pregnancy disorder, the heterogeneity complexity of make it difficult to predict risk develop treatments. Plasma cell-free RNA carries unique information from human tissue may be useful for noninvasive monitoring maternal, placental, fetal dynamics during pregnancy.
Background Mutations in the PHF6 gene were recently described patients with T-cell acute lymphoblastic leukemia and those myeloid leukemia. The present study was designed to determine prevalence of alterations leukemia.Design Methods We analyzed incidence prognostic value mutations 96 Chinese deletions screened by real-time quantitative polymerase chain reaction array-based comparative genomic hybridization. Patients also investigated for NOTCH1, FBXW7, WT1, JAK1 together CALM-AF10,...
Polyunsaturated fatty acids (PUFAs) and their metabolism may be important in normal reproductive function fertility. Associations between physiologic PUFAs pregnancy have not been established women. The purpose of this study was to investigate associations serum levels embryo implantation women undergoing vitro fertilization (IVF). This a prospective cohort conducted 2010 2012. at the Washington University Reproductive Medicine Center. Participants were 200 IVF participating an ongoing...
Abstract Hepatic de novo lipogenesis (DNL) converts carbohydrates into triglycerides and is known to influence systemic lipid homoeostasis. Here, we demonstrate that the zinc finger protein Zbtb20 required for DNL. Mice lacking in liver exhibit hypolipidemia reduced levels of triglycerides, along with impaired hepatic lipogenesis. The expression genes involved glycolysis DNL, including two ChREBP isoforms, decreased livers knockout mice. binds enhances activity ChREBP-α promoter, suggesting...
Technological innovation and increased affordability have contributed to the widespread adoption of genome sequencing technologies in biomedical research. In particular large cancer research consortia embraced next generation sequencing, used technology define somatic mutation landscape multiple types. These studies primarily utilised Illumina HiSeq platforms. this study we performed whole three malignant pleural mesothelioma matched normal samples using a new platform, BGISEQ-500, compared...
Massively-parallel-sequencing, coupled with sample multiplexing, has made genetic tests broadly affordable. However, intractable index mis-assignments (commonly exceeds 1%) were repeatedly reported on some widely used sequencing platforms. Here, we investigated this quality issue BGI sequencers using three library preparation methods: whole genome (WGS) PCR, PCR-free WGS, and two-step targeted PCR. BGI's utilize a unique DNA nanoball (DNB) technology which uses rolling circle replication for...