A5084614665- Gestational Diabetes Research and Management
- Maternal and Perinatal Health Interventions
- Pregnancy and preeclampsia studies
- Maternal and fetal healthcare
- Birth, Development, and Health
- Pregnancy-related medical research
- Neonatal and fetal brain pathology
- Reproductive System and Pregnancy
- Neonatal Respiratory Health Research
- Reproductive Biology and Fertility
- Cardiovascular Issues in Pregnancy
- Preterm Birth and Chorioamnionitis
- Endometriosis Research and Treatment
- Assisted Reproductive Technology and Twin Pregnancy
- Emergency and Acute Care Studies
- Neuroendocrine regulation and behavior
- Endometrial and Cervical Cancer Treatments
- Non-Invasive Vital Sign Monitoring
- Bariatric Surgery and Outcomes
- Estrogen and related hormone effects
- Ovarian function and disorders
- Hemodynamic Monitoring and Therapy
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Trauma and Emergency Care Studies
- Cardiac Arrest and Resuscitation
Washington University in St. Louis
2016-2025
European Brain Council
2024
Robert Wood Johnson Foundation
2023
MedStar Washington Hospital Center
2023
University of Washington
2023
University of Utah
2023
Cleveland Clinic
2023
Harborview Medical Center
2023
American Diabetes Association
2023
Island Institute
2022
The adverse effects of maternal diabetes on embryo development and pregnancy outcomes have recently been shown to occur as early the one-cell zygote stage. hypothesis this study was that maternally inherited mitochondria in oocytes from diabetic mice are abnormal thus responsible part for latency developmental compromise. In ovulated mice, transmission electron microscopy revealed an alteration mitochondrial ultrastructure, quantitative analysis DNA copy number demonstrated increase. levels...
The mechanism responsible for poor reproductive outcomes in type 1 diabetic males is not well understood. In light of new evidence that the Sertoli cells testis secrete insulin, it currently unclear whether subfertility result deficiency pancreatic testicular or both. this study, Akita mouse model, which expresses a mutant, nonfunctional form ins2 testes and pancreas, was used to distinguish between systemic local effects insulin on process spermatogenesis fertility. We determined homozygous...
Impaired oocyte quality has been demonstrated in diabetic mice; however, the potential pathways by which maternal diabetes exerts its effects on are poorly understood. Cumulus cells direct contact with via gap junctions and provide essential nutrients to support development. In this study, we investigated of mitochondrial status cumulus cells. We found an increased frequency fragmented mitochondria, a decreased transmembrane aggregated distribution mitochondria from mice. Furthermore, while...
Recurrent miscarriages affect about 1–2% of couples trying to conceive; however, mechanisms leading this complication are largely unknown. Most studies focus on the early embryo, but proper development and implantation blastocyst also dependent optimal endometrial progression into a receptive state. One key steps in uterine preparation for embryo receptivity, known as decidualization, is differentiation stromal cells (ESCs) decidual cells. During transition, ESCs undergo drastic change...
Free fatty acids (FFAs) are energy substrates for many cell types, but in excess, some FFAs can accumulate nonadipose cells, inducing apoptosis. Also known as lipotoxicity, this phenomenon may play a role the development of obesity-related disease. Obesity is common among reproductive age women and associated with adverse pregnancy fetal outcomes; however, little about effects excess on embryos subsequent development. To address knowledge gap, murine blastocysts were cultured palmitic acid...
We previously identified a coregulator, repressor of estrogen receptor activity (REA), that directly interacts with (ER) and represses ER transcriptional activity. Decreasing the intracellular level REA by using small interfering RNA knockdown or antisense approaches in cells culture resulted significant increase up-regulation estrogen-stimulated genes. To elucidate functional activities vivo, we have used targeted disruption to delete gene mice. The targeting vector eliminated, homologous...
Embryo implantation is a highly synchronized event between an activated blastocyst and receptive endometrium. The success of this process relies on the dynamic interplay estrogen (E2) progesterone (P4), however, details interaction are not entirely clear. Recent data implicate E2 P4 in regulation glucose utilization by affecting facilitative transporter (GLUT) expression. In study we examine GLUT1 expression murine human endometrial stromal cells (ESCs) using primary culture system. We show...
Endometrial stromal cells (ESC) must undergo a hormone-driven differentiation to form decidual as requirement of proper embryo implantation. Recent studies from our laboratory have demonstrated that decidualizing require glucose transporter 1 expression and an increase in use complete this step. The present study focuses on the glucose-dependent molecular metabolic pathways, which are required by ESC for decidualization. Inhibition glycolysis had no effect However, blockade pentose phosphate...
Polyunsaturated fatty acids (PUFAs) and their metabolism may be important in normal reproductive function fertility. Associations between physiologic PUFAs pregnancy have not been established women. The purpose of this study was to investigate associations serum levels embryo implantation women undergoing vitro fertilization (IVF). This a prospective cohort conducted 2010 2012. at the Washington University Reproductive Medicine Center. Participants were 200 IVF participating an ongoing...
To characterize the duration of third stage labor and association with postpartum hemorrhage in a contemporary cohort.We performed secondary analysis cohort 7,121 women who had vaginal delivery at or beyond 37 weeks 0 days gestation single tertiary care center from April 2010 to August 2014. Active management was routinely used during study period. The mean, median, interquartile range, 90th percentile, 95th 99th percentile were calculated. Odds ratios calculated estimate between increased...
Women with polycystic ovary syndrome (PCOS) and hyperandrogenism have altered hormone levels suffer from ovarian dysfunction leading to subfertility. We attempted generate a model of by feeding mice chow supplemented dehydroepiandrosterone (DHEA), an androgen precursor that is often elevated in women PCOS. Treated had ovaries, low ovulation rates, disrupted estrous cycles, levels. Because DHEA inhibitor glucose-6-phosphate dehydrogenase, the rate-limiting enzyme pentose phosphate pathway, we...
Disruptive sleep is a well-established predictor of preterm birth. However, the exact relationship between behavior and birth outcomes remains unknown, in part because prior work has relied on self-reported data. With advent smartwatches, it possible to obtain more reliable accurate data, which can be utilized evaluate impact specific behaviors concert with machine learning. We motion actigraphy data collected from cohort participants undergoing pregnancy, train several learning models based...
Abstract Estrogens regulate multiple activities in breast cancer cells, including proliferation. Whereas these hormones are most commonly known to gene transcription through direct interaction with estrogen receptors (ERs) and specific DNA sequences of target genes, recent studies show that ER also activates a number rapid signaling events initiated at the cell membrane. To study membrane-initiated effects separate them from by nuclear localized human we generated MDA-MB-231 lines have...
This study compares surgical and survival outcomes of women with stage IV uterine serous carcinoma (USC) treated neoadjuvant chemotherapy (NAC) interval cytoreduction to primary cytoreductive surgery (PCS) followed by adjuvant chemotherapy.This retrospective dual cohort included diagnosed USC at a single academic institution. Kruskal-Wallis Fisher exact tests were used compare demographics outcomes. Progression-free (PFS) overall (OS) estimated using Kaplan-Meier methods. Comparison between...
Oxytocin acts through the oxytocin receptor (OXTR) to modulate uterine contractility. We previously identified OXTR genetic variants and showed that, in HEK293T cells, two of protein localized cell surface less than wild-type OXTR. Here, we sought measure more native human myometrial smooth muscle (HMSMC) line on both cell-surface across whole cell, used CRISPR editing add an HA tag endogenous gene for anti-HA measurement. Quantitative flow cytometry revealed that these cells possessed...