A5044533702- Angiogenesis and VEGF in Cancer
- Neuroendocrine regulation and behavior
- Neuroscience of respiration and sleep
- Adipose Tissue and Metabolism
- Receptor Mechanisms and Signaling
- Attachment and Relationship Dynamics
- Lymphatic System and Diseases
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- Mathematical Biology Tumor Growth
- Adipokines, Inflammation, and Metabolic Diseases
- Graphene and Nanomaterials Applications
- Advanced Proteomics Techniques and Applications
- Circadian rhythm and melatonin
- Diet and metabolism studies
- Cancer, Hypoxia, and Metabolism
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pancreatic and Hepatic Oncology Research
- Cardiovascular Disease and Adiposity
- Renal and related cancers
- Evolutionary Psychology and Human Behavior
- Nanoparticle-Based Drug Delivery
- Maternal and fetal healthcare
- Congenital heart defects research
- Nanoplatforms for cancer theranostics
University of Washington
2023-2025
Washington University in St. Louis
2020-2022
ABSTRACT Inadequate angiogenesis in obesogenic adipose tissue (AT) has been implicated disrupted adipogenesis and metabolic disorders. Yet, key cellular molecular regulators of AT remain largely unidentified. This study sought to identify the dysregulated elements within Vascular Endothelial Growth Factor (VEGF) Platelet-Derived (PDGF) systems during obesity progression. We employ a mouse model, comprising both male female mice, investigate changes VEGF/PDGF concentration their receptor...
Oxytocin acts through the oxytocin receptor (OXTR) to modulate uterine contractility. We previously identified OXTR genetic variants and showed that, in HEK293T cells, two of protein localized cell surface less than wild-type OXTR. Here, we sought measure more native human myometrial smooth muscle (HMSMC) line on both cell-surface across whole cell, used CRISPR editing add an HA tag endogenous gene for anti-HA measurement. Quantitative flow cytometry revealed that these cells possessed...
Purpose Receptor tyrosine kinase (RTK) concentrations on the plasma membrane correlate with angiogenic functions in vitro and rodent models. The intracellular RTK pool also regulates receptor availability signaling pathways. Organs have specialized essential to their distinct roles, supporting hypothesis that vary across endothelial cells (ECs) from different organs. Methods Using quantitative flow cytometry human ECs derived dermis, umbilical vein, kidney, liver, brain, we measured...
A recently discovered but unexplored mechanism of angiogenesis regulation is cross-family binding between platelet-derived growth factors (PDGFs) and vascular endothelial factor receptors (VEGFRs), which suggests a novel therapy for addressing dysregulation. This study elucidates the role PDGFs in cell (EC) signaling functions, focusing on VEGFR activation. Using human dermal microvascular ECs (HDMECs) with double knockout PDGFRalpha PDGFRbeta; brain (HBMECs), we show three key findings: (1)...
Abstract Angiogenesis, the formation of new vessels from existing vessels, is mediated by vascular endothelial growth factor (VEGF) and platelet-derived (PDGF). Despite discoveries supporting cross-family interactions between VEGF PDGF families, sharing binding partners them makes it challenging to identify factors that predominantly affect angiogenesis. Systems biology offers promises untangle this complexity. Thus, in study, we developed a mass-action kinetics-based computational model for...
Oxytocin is a potent uterotonic agent administered to nearly all patients during childbirth in the United States. Inadequate oxytocin response can necessitate Cesarean delivery or lead uterine atony and postpartum hemorrhage. Thus, it may be clinically useful identify at risk for poor develop strategies sensitize uterus oxytocin. Previously, we showed that V281M variant receptor (OXTR) gene impairs OXTR trafficking cell surface, leading decreased cells. Here, sought pharmacological...
Vascular endothelial growth factor receptors (VEGFRs) and Axl are receptor tyrosine kinases (RTK) that targeted in ovarian cancer therapy. Two-dimensional monolayer culture three-dimensional spheroids common models for RTK-targeted drug screening: monolayers simple economical while include several genetic histological tumor features. RTK membrane localization dictates signaling response, however, it is not characterized these models. We quantify plasma concentrations show differential...
Abstract Introduction Abnormal angiogenesis is central to vascular disease and cancer, noninvasive biomarkers of origin are needed evaluate patients therapies. Vascular endothelial growth factor receptors (VEGFRs) often dysregulated in these diseases, making them promising biomarkers, but the need for an invasive biopsy has limited biomarker research on VEGFRs. Here, we pioneer a blood approach quantify VEGFR plasma membrane localization two circulating proxies: cells (cECs) progenitor...
The hormone oxytocin is commonly administered during childbirth to initiate and strengthen uterine contractions prevent postpartum hemorrhage. However, patients have wide variation in the dose required for a clinical response. To begin uncover mechanisms underlying this variability, we screened 11 most prevalent missense genetic variants receptor (OXTR) gene. We found that five variants, V45L, P108A, L206V, V281M, E339K, significantly altered oxytocin-induced Ca2+ signaling or β-arrestin...
Obesity is a global health crisis, with its prevalence particularly severe in the United States, where over 42% of adults are classified as obese. driven by complex molecular and tissue-level mechanisms that remain poorly understood. Among these, angiogenesis-primarily mediated vascular endothelial growth factor (VEGF-A)-is critical for adipose tissue expansion but presents unique challenges therapeutic targeting due to intricate regulation. Systems biology approaches have advanced our...
Healthy adipose tissue expansion and metabolism during weight gain require coordinated angiogenesis lymphangiogenesis. These vascular growth processes rely on the endothelial factor (VEGF) family of ligands receptors (VEGFRs). Several studies have shown that controlling by regulating VEGF:VEGFR signaling can be beneficial for treating obesity; however, dysregulated lymphangiogenesis are associated with several chronic inflammation symptoms, including hypoxia, immune cell accumulation,...
Approximately half of U.S. women giving birth annually receive Pitocin, the synthetic form oxytocin (OXT), yet its effective dose can vary significantly. This variability presents safety concerns due to unpredictable responses, which may lead adverse outcomes for both mother and baby. To address need improved dosing, we developed a data-driven mathematical model predict OXT receptor (OXTR) binding. Our study focuses on five prevalent OXTR variants (V45L, P108A, L206V, V281M, E339K) their...
<title>Abstract</title> Approximately half of U.S. women giving birth annually receive Pitocin, the synthetic form oxytocin (OXT), yet its effective dose can vary significantly. To address need for improved dosing, we developed a data-driven mathematical model to predict OXT receptor (OXTR) binding. Our study focuses on five prevalent OXTR variants (V45L, P108A, L206V, V281M, and E339K) their impact OXT–OXTR binding dynamics in human embryonic kidney cells (HEK293T), commonly used...