A5062976329- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Chromosomal and Genetic Variations
- Biofuel production and bioconversion
- Animal Genetics and Reproduction
- Genomics and Chromatin Dynamics
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Gene Regulatory Network Analysis
- Plant tissue culture and regeneration
- Enzyme Catalysis and Immobilization
- Polysaccharides and Plant Cell Walls
- Molecular Biology Techniques and Applications
- Advanced Cellulose Research Studies
- Industrial Vision Systems and Defect Detection
- Bacterial Genetics and Biotechnology
- Biochemical and biochemical processes
- Sesquiterpenes and Asteraceae Studies
- Lignin and Wood Chemistry
- Graph Theory and Algorithms
- DNA Repair Mechanisms
- Insect symbiosis and bacterial influences
Tianjin University
2015-2024
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2015-2022
Ministry of Education of the People's Republic of China
2021
Institute for Systems Biology
2017
INTRODUCTION The Saccharomyces cerevisiae 2.0 project (Sc2.0) aims to modify the yeast genome with a series of densely spaced designer changes. Both synthetic chromosome arm (synIXR) and entirely (synIII) function high fitness in yeast. For synthesis projects, precise engineering physical sequence match specified design is important for systematic evaluation underlying principles. Yeast can maintain nuclear chromosomes as rings, occurring by chance at repeated sequences, although cyclized...
INTRODUCTION Design and construction of an extensively modified yeast genome is a direct means to interrogate the integrity, comprehensiveness, accuracy knowledge amassed by community date. The international synthetic project (Sc2.0) aims build entirely designer, Saccharomyces cerevisiae genome. designed increase stability genetic flexibility while maintaining cell fitness near that wild type. A major challenge for synthesis lies in identifying eliminating fitness-reducing sequence variants...
INTRODUCTION Although much effort has been devoted to studying yeast in the past few decades, our understanding of this model organism is still limited. Rapidly developing DNA synthesis techniques have made a “build-to-understand” approach feasible reengineer on genome scale. Here, we report completion 770-kilobase synthetic chromosome II (synII). SynII was characterized using extensive Trans-Omics tests. Despite considerable sequence alterations, synII virtually indistinguishable from wild...
Compatibility between host cells and heterologous pathways is a challenge for constructing organisms with high productivity or gain of function. Designer yeast incorporating the Synthetic Chromosome Rearrangement Modification by LoxP-mediated Evolution (SCRaMbLE) system provide platform generating genotype diversity. Here we construct genetic AND gate to enable precise control SCRaMbLE method generate synthetic haploid diploid desired phenotypes. The yield carotenoids increased 1.5-fold...
Although the design of synthetic yeast genome Sc2.0 is highly conservative with respect to gene content, deletion several classes repeated sequences and introduction thousands designer changes may affect organization potentially alter cellular functions. We report here Hi-C-determined three-dimensional (3D) conformations chromosomes. The absence repeats leads a smoother contact pattern more precisely tractable chromosome conformations, large-scale genomic globally unaffected by presence...
Synthetic biology tools, such as modular parts and combinatorial DNA assembly, are routinely used to optimise the productivity of heterologous metabolic pathways for biosynthesis or substrate utilisation, yet it is well established that host strain background just important determining productivity. Here we report in vivo genomic rearrangement Saccharomyces cerevisiae yeast with a synthetic chromosome V can rapidly generate new, improved strains genetic backgrounds favourable diverse...
Structural variations (SVs) exert important functional impacts on biological phenotypic diversity. Here we show a ring synthetic yeast chromosome V (ring_synV) can be used to continuously generate complex genomic and improve the production of prodeoxyviolacein (PDV) by applying Synthetic Chromosome Recombination Modification LoxP-mediated Evolution (SCRaMbLE) in haploid cells. The SCRaMbLE ring_synV generates aneuploid strains with increased PDV productivity, identify I, III, VI, XII, XIII,...
Rapid and highly efficient mating-type switching of
Parthenolide, a kind of sesquiterpene lactone, is the direct precursor for promising anti-glioblastoma drug ACT001. Compared with traditional parthenolide source from plant extraction, de novo biosynthesis in microorganisms has potential to make sustainable supply. Herein, an integrated strategy was designed P450 screening, nicotinamide adenine dinucleotide phosphate (NADPH) supply, and endoplasmic reticulum (ER) size rewiring manipulate three P450s regarded as bottleneck production....
Polymerase cycling assembly (PCA) stands out as the predominant method in synthesis of kilobase-length DNA fragments. The design overlapping regions is core factor affecting success rate synthesis. However, there still exists sequences that are challenging to and construct genome Here we proposed a deep learning model based on extensive data discern latent sequence representations with an AUPR 0.805. Utilizing model, developed SmartCut algorithm aimed at designing oligonucleotides enhancing...
With the rapid growth and development of synthetic biology, research in genomics is advancing from genome sequencing to synthesis. In 2009, Professor Jef D. Boeke proposed Synthetic Yeast Genome Project (Sc2.0), which aims synthesize world's first eukaryotic genome. efforts scientists United States, China, Britain, France, Australia, Singapore other countries, a third Saccharomyces cerevisiae chromosomes has now been synthesized. perspectives genomics, we here review recent progress Sc2.0...