A5007384251- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Animal Genetics and Reproduction
- Bacterial Genetics and Biotechnology
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Gene Regulatory Network Analysis
- Genomics and Phylogenetic Studies
- Cassava research and cyanide
- Evolution and Genetic Dynamics
- DNA Repair Mechanisms
- Plant-Microbe Interactions and Immunity
- Immunotherapy and Immune Responses
- Angiogenesis and VEGF in Cancer
- Insect symbiosis and bacterial influences
- Anodic Oxide Films and Nanostructures
- Plant Disease Resistance and Genetics
- Transgenic Plants and Applications
- Potato Plant Research
- Vascular anomalies and interventions
Tianjin University
2015-2025
Coconut Research Institute
2024
Chinese Academy of Tropical Agricultural Sciences
2024
Ministry of Education of the People's Republic of China
2024
Sichuan University
2024
Chengdu University
2024
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2017-2021
Institute for Systems Biology
2017
Fu Jen Catholic University
2013
Cardinal Tien Hospital
2013
INTRODUCTION The Saccharomyces cerevisiae 2.0 project (Sc2.0) aims to modify the yeast genome with a series of densely spaced designer changes. Both synthetic chromosome arm (synIXR) and entirely (synIII) function high fitness in yeast. For synthesis projects, precise engineering physical sequence match specified design is important for systematic evaluation underlying principles. Yeast can maintain nuclear chromosomes as rings, occurring by chance at repeated sequences, although cyclized...
INTRODUCTION Design and construction of an extensively modified yeast genome is a direct means to interrogate the integrity, comprehensiveness, accuracy knowledge amassed by community date. The international synthetic project (Sc2.0) aims build entirely designer, Saccharomyces cerevisiae genome. designed increase stability genetic flexibility while maintaining cell fitness near that wild type. A major challenge for synthesis lies in identifying eliminating fitness-reducing sequence variants...
INTRODUCTION It has long been an interesting question whether a living cell can be constructed from scratch in the lab, goal that may not realized anytime soon. Nonetheless, with advances DNA synthesis technology, complete genetic material of organism now synthesized chemically. Hitherto, genomes several organisms including viruses, phages, and bacteria have designed constructed. These synthetic are able to direct all normal biological functions, capable self-replication production...
Compatibility between host cells and heterologous pathways is a challenge for constructing organisms with high productivity or gain of function. Designer yeast incorporating the Synthetic Chromosome Rearrangement Modification by LoxP-mediated Evolution (SCRaMbLE) system provide platform generating genotype diversity. Here we construct genetic AND gate to enable precise control SCRaMbLE method generate synthetic haploid diploid desired phenotypes. The yield carotenoids increased 1.5-fold...
Although the design of synthetic yeast genome Sc2.0 is highly conservative with respect to gene content, deletion several classes repeated sequences and introduction thousands designer changes may affect organization potentially alter cellular functions. We report here Hi-C-determined three-dimensional (3D) conformations chromosomes. The absence repeats leads a smoother contact pattern more precisely tractable chromosome conformations, large-scale genomic globally unaffected by presence...
SCRaMbLE (Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution) is a genome restructuring technique that can be used in synthetic genomes such as of Sc2.0, the yeast genome, which contains hundreds to thousands strategically positioned loxPsym sites. has been induce rearrangements strains harboring one or more chromosomes, well plasmid DNA vitro vivo. Here we describe collection heterozygous diploid produced mating haploid semisynthetic Sc2.0 native parental...
Abstract The power of synthetic biology has enabled the expression heterologous pathways in cells, as well genome-scale synthesis projects. complexity biological networks makes rational de novo design a grand challenge. Introducing features that confer genetic flexibility is powerful strategy for downstream engineering. Here we develop an vitro method DNA library construction based on structural variation to accomplish this goal. “in SCRaMbLE system” uses Cre recombinase mixed test tube with...
ABSTRACT Synthetic genome evolution provides a dynamic approach for systematically and straightforwardly exploring evolutionary processes. Chromosome Rearrangement Modification by LoxP-mediated Evolution (SCRaMbLE) is an system intrinsic to the synthetic yeast that can rapidly drive structural variations. Here, we detect over 260 000 rearrangement events after SCRaMbLEing of strain harboring 5.5 chromosomes (synII, synIII, synV, circular synVI, synIXR synX). Remarkably, find exhibit specific...
Abstract Synthetic biology confers new functions to hosts by introducing exogenous genetic elements, yet rebuilding complex traits that are based on large-scale information remains challenging. Here, we developed a CRISPR/Cas9-mediated haploidization method bypasses the natural process of meiosis. Based programmed in yeast, further an easy-to-use designated HAnDy (Haploidization-based DNA Assembly and Delivery yeast) enables efficient assembly delivery large DNA, with no need for any fussy...
Strains with increased alkali tolerance have a broad application in industrial, especially for bioremediation, biodegradation, biocontrol and production of bio-based chemicals. A novel synthetic chromosome recombination modification by LoxP-mediated evolution (SCRaMbLE) system has been introduced the yeast genome (Sc 2.0), which enables generation library massive structural variations potentially drives phenotypic evolution. The including deletion, inversion duplication detected within...
As an enabling technique of synthetic biology, the scale DNA assembly largely determines genetic manipulation. However, large technologies are generally cumbersome and inefficient. Here, we developed a YLC (yeast life cycle)-assembly method that enables in vivo iterative by nesting cell-cell transfer assembled cycle yeast mating sporulation. Using this method, successfully hundred-kilobase (kb)-sized endogenous megabase (Mb)-sized exogenous DNA. For each round, over 104 positive colonies per...
Abstract Steroidal glycoalkaloids (SGAs) are major plant defense metabolites against pests, while they considered poisonous in food. The genetic basis that guides negative selection of SGAs production during tomato domestication remains poorly understood. Here, we identify a distal enhancer, GAME Enhancer 1 ( GE1 ), as the key regulator metabolism tomato. recruits MYC2-GAME9 transcriptional complex to regulate expression cluster genes via formation chromatin loops located neighboring DNA...
Betanin is a water-soluble red-violet pigment belonging to the betacyanins family. It has become more and attractive for its natural food colorant properties health benefits. However, commercial production of betanin, typically extracted from red beetroot, faces economic sustainability challenges. Microbial heterologous therefore offers promising alternative. Here, we performed combinatorial engineering plant P450 enzymes precursor metabolisms improve de novo betanin in Saccharomyces...
Among tropical fruit trees, coconut holds significant edible and economic importance. The natural growth of coconuts faces a challenge in the form low temperatures, which is crucial factor among adverse environmental stresses impacting their geographical distribution. Hence, it essential to enhance our comprehension molecular mechanisms through cold stress influences various varieties. We employed analyses leaf morphology physiological traits examine how respond temperatures over 2-hour,...
Synthetic biology revolutionizes our ability to decode and recode genetic systems. The capability reconstruct flexibly manipulate multi-gene systems is critical for understanding cellular behaviors has significant applications in therapeutics. This study aims construct a diverse library of synthetic tunable promoters (STPs) enable flexible control expression mammalian cells. We designed constructed that incorporate both universal activation site (UAS) specific (SAS), enabling multi-level via...
The site-specific recombination system is a versatile tool in genome engineering, enabling controlled DNA inversion or deletion at specific sites to generate genetic diversity. multiplexed system, which preferentially facilitates reverse-oriented rather than same-oriented sites, has not been found eukaryotes. Here, we establish Rci51-5/multi-sfxa101, yeast. Firstly, develop high-throughput screening based on the on/off transcriptional control of multiple markers by inversion. After two...
Rapid and highly efficient mating-type switching of
Abstract Self-propagating drive systems are capable of causing non-Mendelian inheritance. Here, we report a system in yeast referred to as chromosome that eliminates the target via CRISPR-Cas9, enabling transmission desired chromosome. Our results show entire Saccharomyces cerevisiae can be eliminated efficiently through only one double-strand break around centromere CRISPR-Cas9. As proof-of-concept experiment this CRISPR-Cas9 system, synthetic X is completely eliminated, and counterpart...
Summary Circulating endothelial progenitor cells (EPCs) are bone marrow‐derived required for repair. A low EPC number can be considered as an independent predictor of dysfunction and future cardiovascular events. Recent evidence shows that patients with hypogonadal symptoms without other confounding risk factors have a circulating (PCs) EPCs, thus highlighting the role testosterone in proliferation differentiation EPCs. Here, we investigate if replacement therapy (TRT) increase men late...
Genome-scale gene knockout is an important approach to the study of global genetic interactions. SCRaMbLEing synthetic yeast chromosomes provides efficient way generate random deletion mutants. Here, we demonstrate use SCRaMbLE explore lethal First, all essential genes chromosome III (chrIII) were clustered in a centromeric plasmid. We found that three types reorganized chrIII had similar transcriptional levels. Further, (synIII) with supplementary enables large chromosomal regions....