Limonene, a plant-derived natural cyclic monoterpene, is widely used in the pharmaceutical, food, and cosmetics industries. The conventional limonene biosynthetic (CLB) pathway engineered Saccharomyces cerevisiae consists of heterologous synthase (LS) using endogenous substrate geranyl diphosphate (GPP) suffers from poor production limonene. In this study, we report on an orthogonal engineering strategy S. for improving We reconstructed (OLB) composed SlNDPS1 that catalyzes IPP DMAPP to NPP...

Limonene, a cyclic monoterpene, is widely used in food and cosmetics industries as well agriculture. In the work described herein, employing systematic optimization strategy, we constructed an efficient platform for producing limonene via heterologous mevalonate pathway Escherichia coli. By site-directed mutation of EfMvaS tuning initial translation EfMvaE EfMvaSA110G through ribosome binding site engineering, upstream module overproducing was obtained. Expression MmMK with ScPMK, ScPMD,...

Betanin is a water-soluble red-violet pigment belonging to the betacyanins family. It has become more and attractive for its natural food colorant properties health benefits. However, commercial production of betanin, typically extracted from red beetroot, faces economic sustainability challenges. Microbial heterologous therefore offers promising alternative. Here, we performed combinatorial engineering plant P450 enzymes precursor metabolisms improve de novo betanin in Saccharomyces...

Purpose This study empirically examines the impact of announcements on supply chain strategic collaboration (SCSC) companies' shareholder value. Design/methodology/approach analyzes changes in value companies listed China based data 208 SCSC announcements. The signaling theory is applied to determine correlation among and market. An event used estimate stock market reaction common model estimates abnormal returns after event. least squares method regression calculate parameter Findings There...

Purpose The purpose of this study is to examine the impact implementation supply chain strategic collaboration (SCSC) on companies’ operating performance. Design/methodology/approach Based 181 SCSC announcements listed companies in China, analyzes changes performance sample 20 quarters after announcement. different metrics for firms are compared against their before This uses a self-control model based historical and combination adjustment percentage level measure changes. Findings helps...

Purpose The purpose of this paper is to analyze the influencing factors new logistics service product design (NLSPD) in China establish a theoretical framework for future development industry. Design/methodology/approach adopts multi-case study method based on sample four Chinese enterprises, which authors consider maturity (LSM), distinct characteristic enterprises. Findings NLSPD directly related degree supply–demand matching (SDM) and LSM. Customer demand, capability peer competition...

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The performance of the logistics service supply chain (LSSC) is not only affected by external demand changes, but also decision-making behaviours LSSC members, including integrators (LSI) and functional providers (FLSP). This paper establishes a structural equation model for assessing relationships among updating, performance. validated questionnaire data from China using SmartPLS method. There are many important conclusions. Firstly, updating acting as an intermediary variable does have...

This paper studies the effect of M&A announcements on shareholder value listed companies from Chinese logistics industry. Using event study method, this research examines market reaction to 181 initial successful M&As between 2000 and 2016. Some important findings are obtained. Firstly, we find a significantly positive mean (1.125%) for acquiring firms. Secondly, is more firms with higher level service integration. The subsector concentration, abnormal returns. mergers involving vertical...

According to the related data from 1999 2007, this paper applies gray prediction model forecast total enrollment of students adult higher education in China 2008 2012 order predict development trend Chinese education; then, it chose some indexes such as education, GDP per capita, all households consumption expenditure, proportion employees whose educational level is above college employed people and so forth analyze relevant factors influencing based on grey correlation analysis.

Supply chain strategic collaboration (SCSC) is an important means for enterprises to cultivate competitive advantage in the supply chain, but this will also be accompanied by risks. This paper focuses on impact of termination SCSC market value. We select samples from Chinese listed companies test stock reaction announcement termination. The results show that mean (median) cumulative −1.97% (−0.68%) day and after announcement, significantly different 0 at level 1% (5%). find shorter duration...

This paper studies the effect of M&A announcements on shareholder value listed companies from Chinese logistics industry. Using event study method, this research examines market reaction to 181 initial successful M&As between 2000 and 2016. Some important findings are obtained. Firstly, we find a significantly positive mean (1.125%) for acquiring firms. Secondly, is more firms with higher level service integration. The subsector concentration, abnormal returns. mergers involving vertical...