A5057428911- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Plant biochemistry and biosynthesis
- Fungal and yeast genetics research
- Microbial Community Ecology and Physiology
- Fermentation and Sensory Analysis
- Bacterial Genetics and Biotechnology
- Microbial bioremediation and biosurfactants
- Microbial metabolism and enzyme function
- Microbial Natural Products and Biosynthesis
- Bacteriophages and microbial interactions
- biodegradable polymer synthesis and properties
- Microplastics and Plastic Pollution
- Genomics and Phylogenetic Studies
- Microbial Fuel Cells and Bioremediation
- Protist diversity and phylogeny
- Plant Gene Expression Analysis
- Metabolomics and Mass Spectrometry Studies
- Gene Regulatory Network Analysis
- CRISPR and Genetic Engineering
- Bioeconomy and Sustainability Development
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Microbial Metabolites in Food Biotechnology
- Wastewater Treatment and Nitrogen Removal
Tianjin University
2016-2025
Ministry of Education of the People's Republic of China
2011-2024
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2014-2022
Zhejiang A & F University
2018-2019
Guangxi University of Science and Technology
2019
INTRODUCTION The Saccharomyces cerevisiae 2.0 project (Sc2.0) aims to modify the yeast genome with a series of densely spaced designer changes. Both synthetic chromosome arm (synIXR) and entirely (synIII) function high fitness in yeast. For synthesis projects, precise engineering physical sequence match specified design is important for systematic evaluation underlying principles. Yeast can maintain nuclear chromosomes as rings, occurring by chance at repeated sequences, although cyclized...
Poly(ethylene terephthalate) hydrolase (PETase) from Ideonella sakaiensis exhibits a strong ability to degrade poly(ethylene (PET) at room temperature, and is thus regarded as potential tool solve the issue of polyester plastic pollution. Therefore, we explored interaction between PETase substrate (a dimer PET monomer ethylene terephthalate, 2PET), using model its substrate. In this study, focused on six key residues around substrate-binding groove in order create novel high-efficiency...
A synbio strategy for efficient sugar-to-electricity conversion.
Astaxanthin is a natural carotenoid pigment with tremendous antioxidant activity and great commercial value. Microbial production of astaxanthin via metabolic engineering has become promising alternative. Although efforts have been conducted by tuning the heterologous modules precursor pools, yields in these non-carotenogenic microorganisms were still unsatisfactory for commercialization, indicating that addition to targeted tailoring limited targets guided rationally design, combining more...
Abstract While plastics bring convenience to industrial production and daily life, serious environmental problems are always accompanied. Polyurethane is an important polymeric material with versatile applications, but it also accounts for severe plastic contamination worldwide. Since difficult polyurethanes be completely degraded under natural conditions, the discovery of polyurethane‐degrading microorganisms correlative ‘polyurethanase’ enzymes brings hope polyurethane biodegradation....
Fengycin has great potential for applications in biological control because of its biosafety and degradability. In this study, the addition exogenous precursors increased fengycin production by Bacillus subtilis. Corynebacterium glutamicum was engineered to produce high levels (Thr, Pro, Val, Ile) promote biosynthesis fengycin. Furthermore, recombinant C. Yarrowia lipolytica providing amino acid fatty were co-cultured improve B. subtilis a three-strain artificial consortium, which 2100...
Identification of efficient key enzymes in biosynthesis pathway and optimization the fitness between functional modules chassis are important for improving production target compounds. In this study, taxadiene was firstly constructed yeast by transforming ts gene overexpressing erg20 thmgr. Then, catalytic capabilities six different geranylgeranyl diphosphate synthases (GGPPS), enzyme mevalonic acid (MVA) catalyzing famesyl (FPP) to (GGPP), were predicted using enzyme-substrate docking...
In the industry, conventional two-step fermentation method was used to produce 2-keto-L-gulonic acid (2-KGA), precursor of vitamin C, by three strains, namely, Gluconobacter oxydans, Bacillus spp. and Ketogulonicigenium vulgare. Despite its high production efficiency, long incubation period an additional second sterilization process inhibit further development. Therefore, we aimed reorganize a synthetic consortium G. oxydans K. vulgare for one-step 2-KGA enhance symbiotic interaction between...
Polyethylene terephthalate (PET) biodegradation is regarded as an environmentally friendly degradation method. In this study, artificial microbial consortium composed of Rhodococcus jostii , Pseudomonas putida and two metabolically engineered Bacillus subtilis was constructed to degrade PET. First, a two-species with B. that could secrete PET hydrolase (PETase) monohydroxyethyl (MHETase), respectively; it 13.6% (weight loss) the film within 7 days. A three-species further obtained by adding...
Fengycin is a lipopeptide with broad-spectrum antifungal activity. However, its low yield limits commercial application. Therefore, we iteratively edited multiple target genes associated fengycin synthesis by combinatorial metabolic engineering. The ability of Bacillus subtilis 168 to manufacture lipopeptides was restored, and the titer 1.81 mg/L. production further increased 174.63 mg/L after knocking out pathways surfactin bacillaene replacing native promoter (PppsA) Pveg promoter....
Iturin A biosynthesis has garnered considerable interest, yet bottlenecks persist in its low productivity wild strains and the ability to engineer Bacillus amyloliquefaciens producers. This study reveals that deleting endogenous plasmid, plas1, from wild-type B. HM618 notably enhances iturin synthesis, likely related effect of Rap phosphatase gene within plas1. Furthermore, inactivating phosphatase-related genes (rapC, rapF, rapH) genome strain also improved level specific while reducing...
Metabolic profiling was carried out to investigate the interactive effects of three representative inhibitors (furfural, phenol, and acetic acid) in lignocellulosic hydrolysate on Saccharomyces cerevisiae during ethanol fermentation. Our results revealed that exhibited significantly synergistic growth, fermentation, some metabolites yeast. Acetic acid exerted most severe yeast combination inhibitors, enhancing amino acids metabolism inhibiting central carbon metabolism. The cells by were...