A5002279999- Microbial Metabolism and Applications
- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Fungal and yeast genetics research
Imperial College London
2016-2018
Synthetic biology tools, such as modular parts and combinatorial DNA assembly, are routinely used to optimise the productivity of heterologous metabolic pathways for biosynthesis or substrate utilisation, yet it is well established that host strain background just important determining productivity. Here we report in vivo genomic rearrangement Saccharomyces cerevisiae yeast with a synthetic chromosome V can rapidly generate new, improved strains genetic backgrounds favourable diverse...
Abstract Fungi are a valuable source of enzymatic diversity and therapeutic natural products including antibiotics. Here we engineer the baker’s yeast Saccharomyces cerevisiae to produce secrete antibiotic penicillin, beta-lactam nonribosomal peptide, by taking genes from filamentous fungus directing their efficient expression subcellular localization. Using synthetic biology tools combined with long-read DNA sequencing, optimize productivity 50-fold bioactive yields that allow spent S....
Abstract Fungi are a valuable source of enzymatic diversity and therapeutic natural products including antibiotics. By taking genes from filamentous fungus directing their efficient expression subcellular localisation, we here engineer the baker’s yeast Saccharomyces cerevisiae to produce secrete antibiotic penicillin, beta-lactam nonribosomal peptide. Using synthetic biology tools combined with long-read DNA sequencing, optimise productivity by 50-fold bioactive yields that allow spent S....