A5100310852- Single-cell and spatial transcriptomics
- Reproductive Biology and Fertility
- Assisted Reproductive Technology and Twin Pregnancy
- Lung Cancer Research Studies
- Genomics and Phylogenetic Studies
- Inhalation and Respiratory Drug Delivery
- Reproductive Health and Technologies
- Microbial Community Ecology and Physiology
- Acute Lymphoblastic Leukemia research
- Hereditary Neurological Disorders
- Epigenetics and DNA Methylation
- Hydrology and Watershed Management Studies
- Soil erosion and sediment transport
- RNA modifications and cancer
- Plant-Microbe Interactions and Immunity
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Neurological diseases and metabolism
- Animal Virus Infections Studies
- Cancer-related gene regulation
- Gene expression and cancer classification
- Advanced Statistical Process Monitoring
- Environmental Changes in China
- Bacteriophages and microbial interactions
- Genetics and Neurodevelopmental Disorders
Tsinghua University
2022-2025
Wenzhou Medical University
2022-2025
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2022-2025
Guangdong Medical College
2023-2024
UCSI University
2022-2024
Chinese Academy of Sciences
2006-2024
Zhanjiang Experimental Station
2021-2024
Institute of Hydrobiology
2023-2024
State Key Laboratory of Freshwater Ecology and Biotechnology
2024
University of Chinese Academy of Sciences
2012-2024
Onconase, or P-30, is a protein initially purified from extracts of Rana pipiens oocytes and early embryos based upon its anticancer activity both in vitro vivo. It basic single-chain with an apparent molecular mass 12,000 daltons homologous to RNase A. In cultured 9L glioma cells, onconase inhibits synthesis IC50 about 10(-7) M. The inhibition correlates cell death determined by clonogenic assays. 125I-Labeled binds specific sites on cells. Scatchard analysis the binding data shows that...
Pollen- and seed-mediated transgene flow is a concern in plant biotechnology. We report here highly efficient 'genetically modified (GM)-gene-deletor' system to remove all functional transgenes from pollen, seed or both. With the three pollen- and/or seed-specific gene promoters tested, phage CRE/loxP yeast FLP/FRT alone was inefficient excising tobacco pollen seed, with no transgenic event having 100% efficiency. When loxP-FRT fusion sequences were used as recognition sites, simultaneous...
The Tibetan Plateau induces and enhances the Asian monsoon that influences plateau itself, East China even whole of Asia. Pursuing changing history its impact in this environment-sensitive area is a key for understanding climatic changes. Here, we present initial results from core sediments Nam Co, central Tibet, to elucidate Holocene environment changes area. studied lake (Nam Co) second largest lake, with an elevation 4718 m, water 2015 km 2 catchment 10 610 . By using echo-seismic...
We report a broader than previously appreciated clinical spectrum for hereditary sensory and autonomic neuropathy type 1E (HSAN1E) potential pathogenic mechanism DNA methyltransferase (DNMT1) mutations. The presentations genetic characteristics of nine newly identified HSAN1E kinships (45 affected subjects) were investigated. Five novel mutations DNMT1 discovered; p.C353F, p.T481P, p.P491L, p.Y524D p.I531N, all within the target-sequence domain, two (p.T481P, p.P491L) arising de novo....
Abstract Background Genome-wide methylation profiling has led to more comprehensive insights into gene regulation mechanisms and potential therapeutic targets. Illumina Human Methylation BeadChip is one of the most commonly used genome-wide platforms. Similar other microarray experiments, data susceptible various technical artifacts, particularly batch effects. To date, little attention been given issues related normalization effect correction for this kind data. Methods We evaluated three...
To improve understanding of TRPV4-associated axonal Charcot-Marie-Tooth (CMT) neuropathy phenotypes and their debated pathologic mechanism.A total 17 CMT2C phenotypic families with vocal cord diaphragmatic involvement 36 clinically undifferentiated CMT2 subjects underwent sequencing analysis the coding region TRPV4. Functional studies mutant proteins were performed using transiently transfected cells for TRPV4 subcellular localization, basal stimulated Ca(2+) channel analysis, cell viability...
Mutations in Charcot-Marie-Tooth disease (CMT) genes are the cause of rare familial forms polyneuropathy. Whether allelic variability CMT is also associated with common polyneuropathy-considered "acquired" medical parlance-is unknown. Chemotherapy-induced peripheral neuropathy (CIPN) occurs commonly cancer patients and individually unpredictable. We used CIPN as a clinical model to investigate association non-CMT polyneuropathy genes.A total 269 neurologically asymptomatic were enrolled...
Under consecutive monoculture, the biomass and quality of Pseudostellaria heterophylla declines significantly. In this study, a three-year field experiment was conducted to identify typical growth inhibition effects caused by extended monoculturing P. heterophylla. Deep pyrosequencing used examine changes in structure composition soil fungal community along gradient monoculture. The results revealed distinct separation between newly planted plot two-year, monocultured plots. Shannon Simpson...
Abstract Profiling spatial variations of cellular composition and transcriptomic characteristics is important for understanding the physiology pathology tissues. Spatial transcriptomics (ST) data depict gene expression but currently dominating high-throughput technology yet not at single-cell resolution. Single-cell RNA-sequencing (SC) provide information level lack information. Integrating these two types would be ideal revealing landscapes We develop method STEM (SpaTially aware EMbedding)...
Abstract Purpose: We have previously mapped a major susceptibility locus influencing familial lung cancer risk to chromosome 6q23-25. However, the causal gene at this remains undetermined. In study, we further refined identify single candidate gene, by fine mapping using microsatellite markers and association studies high-density nucleotide polymorphisms (SNP). Experimental Design: Six multigenerational families with five or more affected members were chosen for fine-mapping 6q linkage...
Mutations in the PKHD1 gene, which encodes fibrocystin, cause autosomal recessive polycystic kidney disease (ARPKD). Unfortunately, lack of specific antibodies to mouse protein impairs study splicing, post-translational processing, shedding, and temporal spatial expression endogenous fibrocystin at cellular subcellular level. Here, we report using a knock-in strategy generate null Pkhd1 strain that expresses along with two SV5-Pk epitope tags engineered in-frame into third exon, immediately...
Mutations in DNA methyltransferase 1 (DNMT1) have been identified 2 autosomal dominant syndromes: 1) hereditary sensory autonomic neuropathy with dementia and hearing loss (HSAN1E); 2) cerebellar ataxia, deafness, narcolepsy. Both syndromes mutations targeting sequence (TS) domain (exons 20-21), which is important mediating substrate binding to the DNMT1 catalytic domain. Frontal lobe hypometabolism has documented an HSAN1E family, but memory primary cognitive complaint. The chromosomal...
DNA methyltransferase 1 (DNMT1) is essential for methylation, gene regulation and chromatin stability. We previously discovered DNMT1 mutations cause hereditary sensory autonomic neuropathy type with dementia hearing loss (HSAN1E; OMIM 614116). HSAN1E the first adult-onset neurodegenerative disorder caused by a defect in gene. patients appear clinically normal until young adulthood, then begin developing characteristic symptoms involving central peripheral nervous systems. Some also develop...
<h3>Objective:</h3> To assess the efficiency of target-enrichment next-generation sequencing (NGS) with copy number assessment in inherited neuropathy diagnosis. <h3>Methods:</h3> A 197 polyneuropathy gene panel was designed to for mutations 93 patients or idiopathic without known genetic cause. We applied our novel variation algorithm on NGS data, and validated identified using CytoScan (Affymetrix). Cost efficacy this targeted approach compared earlier evaluations. <h3>Results:</h3>...
The production and quality of Rehmannia glutinosa can be dramatically reduced by replant disease under consecutive monoculture. root-associated microbiome, also known as the second genome plant, was investigated to understand its impact on plant health. Culture-dependent culture-independent pyrosequencing analysis applied assess shifts in soil bacterial communities rhizosphere rhizoplane results show that microbiome (including microbiomes) significantly impacted rhizocompartments Consecutive...
Abstract The Mariana Trench is the deepest location on earth and harbors unique microbial communities as evidenced by 16S rRNA gene amplicon metagenomic sequencing. Obtaining culturable microorganisms from will contribute to a further understanding of hadal biogeochemical processes act reservoir with potential applications. Here, 825 bacterial strains, identified sequencing, were isolated 12 water depths (0–10,400 m) 2216E R2A media at 4 °C or 28 four cruises during 2015–2017. These bacteria...
Abstract. Lake surface water temperature (LSWT) is a critical physical property of the aquatic ecosystem and an evident indicator climate change. By combining strengths satellite-based observation modeling, we have produced integrated daily LSWT for 160 lakes across Tibetan Plateau where in situ limited. The MODIS-based lake-wide mean dataset includes daytime, nighttime, period 2000–2017. used to calibrate simplified physically based model (i.e., modified air2water model), upon which...