A5014223975- Microbial Natural Products and Biosynthesis
- Biochemical and Structural Characterization
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Digestive system and related health
- Cancer Research and Treatments
- Protein Structure and Dynamics
- Amino Acid Enzymes and Metabolism
- Genomics and Phylogenetic Studies
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Membrane Separation Technologies
- Lipid Membrane Structure and Behavior
- Chemical Synthesis and Analysis
- Venomous Animal Envenomation and Studies
- Carbohydrate Chemistry and Synthesis
- Antimicrobial Peptides and Activities
- Wnt/β-catenin signaling in development and cancer
- Peptidase Inhibition and Analysis
- Ion Channels and Receptors
- RNA modifications and cancer
- Lanthanide and Transition Metal Complexes
- Advanced MRI Techniques and Applications
National University of Singapore
2024-2025
Yong In University
2025
The University of Queensland
2014-2024
National Imaging Facility
2017-2024
Peking University
2014-2023
China-Japan Friendship Hospital
2023
Center for Life Sciences
2023
Xidian University
2023
South China Agricultural University
2021-2022
Suzhou University of Science and Technology
2019-2021
Abstract With the emergence of multidrug-resistant bacteria, antimicrobial peptides (AMPs) offer promising options for replacing traditional antibiotics to treat bacterial infections, but discovering and designing AMPs using methods is a time-consuming costly process. Deep learning has been applied de novo design address AMP classification with high efficiency. In this study, several natural language processing models were combined identify AMPs, i.e. sequence generative adversarial nets,...
Phox homology (PX) domains are membrane interacting that bind to phosphatidylinositol phospholipids or phosphoinositides, markers of organelle identity in the endocytic system. Although many PX canonical endosome-enriched lipid PtdIns3P, others interact with alternative and a precise understanding how these specificities arise has remained elusive. Here we systematically screen all human for their phospholipid preferences using liposome binding assays, biolayer interferometry isothermal...
Structural studies of proteins and protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy can be greatly enhanced site-specific attachment lanthanide ions to create paramagnetic centers. In particular, pseudocontact shifts (PCS) generated lanthanides contain important unique long-range structure information. Here, we present a high-affinity binding tag that attached single cysteine residues proteins. The new has many advantageous features are not available in this...
The box jellyfish, Chironex fleckeri, is the largest and most dangerous cubozoan jellyfish to humans. It produces potent rapid-acting venom its sting causes severe localized systemic effects that are potentially life-threatening. In this study, a combined transcriptomic proteomic approach was used identify C. fleckeri proteins elicit toxic in envenoming.More than 40,000,000 Illumina reads were de novo assemble ∼ 34,000 contiguous cDNA sequences 20,000 predicted based on homology searches,...
Disulfide-rich cyclic peptides have generated great interest in the development of peptide-based therapeutics due to their exceptional stability toward chemical, enzymatic, or thermal attack. In particular, they been used as scaffolds onto which bioactive epitopes can be grafted take advantage favorable biophysical properties disulfide-rich peptides. To date, most commonly method for head-to-tail cyclization has native chemical ligation. recent years, however, enzyme-mediated become a...
Abstract Purpose: NAT10 (N-acetyltransferase 10) is a nucleolar protein, but may show subcellular redistribution in colorectal carcinoma. In this study, we evaluated membranous staining of carcinoma and its clinical implications, explored the mechanism regulation redistribution. Experimental Design: The expression NAT10, β-catenin, E-cadherin, GSK-3β were by immunohistochemistry 222 cases Regulation influence on cell motility analyzed with inhibitors GSK-3β, transfection wild-type or...
Abstract Many natural products contain the hexahydropyrrolo[2, 3- b ]indole (HPI) framework. HPI containing chemicals exhibit various biological activities and distinguishable structural arrangement. This complexity renders chemical synthesis very challenging. Here, through investigating biosynthesis of a naturally occurring C 3 -aryl HPI, naseseazine (NAS-C), we identify P450 enzyme (NascB) reveal that NascB catalyzes radical cascade reaction to form intramolecular intermolecular...
Tetrocarcin A (TCA), produced by Micromonospora chalcea NRRL 11289, is a spirotetronate antibiotic with potent antitumor activity and versatile modes of action. In this study, the biosynthetic gene cluster TCA was cloned localized to 108-kb contiguous DNA region. silico sequence analysis revealed 36 putative genes that constitute (including 11 for unusual sugar biosynthesis, 13 aglycone formation, 4 glycosylations) allowed us propose pathway TCA. The formation D-tetronitrose, L-amicetose,...
Natural products are critical for drug discovery and development; however their is challenged by the wide inactivation or silence of microbial biosynthetic pathways. Currently strategies targeting this problem mainly concentrated on chromosome dissembling, transcription, translation-stage regulations as well chemical stimulation. In study, we developed a novel approach to awake cryptic/silenced pathways through augmentation conserved protein modification step-phosphopantetheinylation carrier...
Abstract Bacterial heterodimeric tryptophan-containing diketopiperazines (HTDKPs) are a growing family of bioactive natural products. They challenging to prepare by chemical routes due the polycyclic and densely functionalized backbone. Through functional characterization investigation, we herein identify three related HTDKP-forming cytochrome P450s (NasbB, Nas S1868 F5053 ) reveal four critical residues (Qln65, Ala86, Ser284 Val288) that control their regio- stereo-selectivity generate...
As reported herein, the waterborne polymers poly(glycidyl methacrylate-co-poly(ethylene glycol) methyl ether methacrylate) P(GMA-co-mPEGMA) and polyethyleneimine (PEI) were used to prepare multipurpose polyvinylidene fluoride (PVDF) membranes via a direct spray-coating method. PEI alternately sprayed onto PVDF membrane yield stable cross-linked copolymer coatings. The successful coating of surface was verified by scanning electron microscopy, attenuated total reflectance-Fourier transform...
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a newly discovered lipid class known for their potential anti-inflammatory and insulin-sensitizing properties. A sustainable efficient synthesis route is essential to realize the FAHFAs enable cost-effective, large-scale production. Enzymatic synthesis, favored its scalability environmental impact, preferred approach. Candida (Moesziomyces) antarctica lipase (CalA), previously thermostability limited ability catalyze FAHFA esterification,...
Keratoderma-hypotrichosis-leukonychia totalis syndrome (KHLS) is an extremely rare, autosomal-dominant disorder characterized by severe skin hyperkeratosis, congenital alopecia and leukonychia totalis. The genetic defect underlying KHLS remained undetermined. By performing whole-exome sequencing in a family with KHLS, we identified heterozygous mutation (c.23G>T [p.Gly8Val]) GJA1, which cosegregated the phenotype family. In additional affected individual, also found identical de novo was...
Abstract Cyclic α-conotoxin Vc1.1 (cVc1.1) is an orally active peptide with analgesic activity in rat models of neuropathic pain. It has two disulfide bonds, which can have three different connectivities, one the native and form. In this study we used computational modeling nuclear magnetic resonance to design a disulfide-deleted mutant cVc1.1, [C2H,C8F]cVc1.1, larger hydrophobic core than cVc1.1 and, potentially, additional surface salt bridge interactions. The new variant, hcVc1.1, similar...
Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive tumor of the bile duct, and significant public health problem in East Asia, where it associated with infection by parasite Opisthorchis viverrini. ICC often detected at an advanced stage poor prognosis, making biomarker for early detection priority. We have comprehensively profiled miRNA expression levels tissue using small RNA-Seq validated these profiles quantitative PCR on matched plasma samples. Distinct were increasing...
The airway epithelium is exposed to a range of irritants in the environment that are known trigger inflammatory response such as asthma. Transient receptor potential vanilloid 1 (TRPV1) Ca 2+ ‐permeable cation channel critical for detecting noxious stimuli by sensory neurons. Recently increasing evidence suggests TRPV1 also crucially involved pathophysiology asthma on human. Here we report epithelial cells activation potently induces allergic cytokine thymic stromal lymphopoietin (TSLP)...
Proteogenomic re-annotation and mRNA splicing information can lead to the discovery of various protein forms for eukaryotic model organisms like rat. However, detection novel proteoforms using mass spectrometry proteomics data remains a formidable challenge. We developed EuGenoSuite, an open source multiple algorithmic proteomic search tool utilized it in our in-house integrated transcriptomic-proteomic pipeline facilitate automated proteogenomic analysis. Using four pipelines (integrated...