A5012567065- Genetic Neurodegenerative Diseases
- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Erythrocyte Function and Pathophysiology
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Nuclear Structure and Function
- Cellular transport and secretion
- Cardiac Ischemia and Reperfusion
- Iron Metabolism and Disorders
- Childhood Cancer Survivors' Quality of Life
- Fuel Cells and Related Materials
- Adipose Tissue and Metabolism
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Stroke Rehabilitation and Recovery
- Ubiquitin and proteasome pathways
- Esophageal and GI Pathology
- Cardiac Imaging and Diagnostics
- Gastrointestinal disorders and treatments
- Caveolin-1 and cellular processes
- Hereditary Neurological Disorders
- Force Microscopy Techniques and Applications
KIIT University
2021
Indian Institute of Technology Bhubaneswar
2021
The University of Texas Southwestern Medical Center
2018-2020
Southwestern Medical Center
2018-2020
University of Arizona
2020
Indian Institute of Science Education and Research Kolkata
2018-2019
Boston Children's Hospital
2009
Harvard University
2009
Madras Medical Mission
2006
Phox homology (PX) domains are membrane interacting that bind to phosphatidylinositol phospholipids or phosphoinositides, markers of organelle identity in the endocytic system. Although many PX canonical endosome-enriched lipid PtdIns3P, others interact with alternative and a precise understanding how these specificities arise has remained elusive. Here we systematically screen all human for their phospholipid preferences using liposome binding assays, biolayer interferometry isothermal...
Lipid droplets (LDs) are nutrient reservoirs used by cells to maintain homeostasis. Nascent form on the endoplasmic reticulum (ER) and grow following an influx of exogenous fatty acids (FAs). The budding LDs requires extensive ER–LD crosstalk, but how this is regulated remains poorly understood. Here, we show that sorting nexin protein Snx14, ER-resident associated with cerebellar ataxia SCAR20, localizes contacts FA treatment, where it promotes LD maturation. Using proximity-based APEX...
Lipid droplets (LDs) serve as cytoplasmic reservoirs for energy-rich fatty acids (FAs) stored in the form of triacylglycerides (TAGs). During nutrient stress, yeast LDs cluster adjacent to vacuole/lysosome, but how this LD accumulation is coordinated remains poorly understood. The ER protein Mdm1 a molecular tether that plays role clustering during depletion, its mechanism function unknown. Here, we show associates with through hydrophobic N-terminal region, which sufficient demarcate sites...
Cholesterol depletion by methyl-β-cyclodextrin (MβCD) remodels the plasma membrane’s mechanics in cells and its interactions with underlying cytoskeleton, whereas red blood cells, it is also known to cause lysis. Currently it’s unclear if MβCD alters membrane tension or only enhances membrane-cytoskeleton interactions—and how this relates cell We map height fluctuations single observe that reduces temporal robustly but flattens spatial undulations slightly. Utilizing models explicitly...
Mutations in SNX14 cause the autosomal recessive cerebellar ataxia 20 (SCAR20). generally result loss of protein although several coding region deletions have also been reported. Patient-derived fibroblasts show disrupted autophagy, but precise function is unknown. The yeast homolog, Mdm1, functions endoplasmic reticulum (ER)-lysosome/vacuole inter-organelle tethering, functional conservation mammals still required. Here, we that alters does not block autophagic flux. In addition, find an...
The natural history of caliceal diverticula in children is unknown. We review our series with to examine the presentation, and management.We retrospectively reviewed records 22 presenting between 1983 2006. All pertinent clinical data were recorded, including demographics, imaging studies, treatment outcome.A total had 23 diverticula. There 14 girls 8 boys who presented at a mean age 5.4 +/- 3.1 years (range 0.2 12). At presentation 10 patients febrile urinary tract infection, 2 hematuria,...
Fatty acids (FAs) are central cellular metabolites that contribute to lipid synthesis, and can be stored or harvested for metabolic energy. Dysregulation in FA processing storage causes toxic accumulation altered membrane compositions contributes neurological disorders. Saturated lipids particularly detrimental cells, but how saturation levels maintained remains poorly understood. Here, we identify the cerebellar ataxia spinocerebellar ataxia, autosomal recessive 20 (SCAR20)-associated...
Abstract Mutations in the SNX14 gene cause spinocerebellar ataxia, autosomal recessive 20 (SCAR20) both humans and dogs. Studies implicating phenotypic consequences of mutations to be subcellular disruption autophagy lipid metabolism have been limited vitro investigation patient-derived dermal fibroblasts, laboratory engineered cell lines developmental analysis zebrafish morphants. homologues Snz ( Drosophila ) Mdm1 (yeast) also conducted, demonstrated an important biochemical role during...
Abstract Cholesterol depletion in cells by MβCD remodels the plasma membrane’s mechanics and its interactions with underlying cytoskeleton. Decoupling two effects studying various alterations to mechanical parameters is important for understanding cholesterol’s role cellular response stress. By mapping membrane height fluctuations single cells, we report that treatment reduces temporal flattens out – but does not supress activity-driven fluctuations. We find tension increase contributes most...
Abstract Fatty acids (FAs) are central cellular metabolites that contribute to lipid synthesis, and can be stored or harvested for metabolic energy. Dysregulation in FA processing storage causes toxic accumulation altered membrane compositions contributes neurological disorders. Saturated lipids particularly detrimental cells, but how saturation levels maintained remains poorly understood. Here, we identify the cerebellar ataxia SCAR20-associated protein Snx14, an endoplasmic reticulum...
Summary Adipocytes store nutrients as lipid droplets (LDs), but how they organize their LD stores to balance uptake, storage, and mobilization remains poorly understood. Here, using Drosophila fat body (FB) adipocytes we characterize spatially distinct populations that are maintained by different pools. We identify peripheral LDs (pLDs) make close contact with the plasma membrane (PM) lipophorin-dependent trafficking. pLDs from larger cytoplasmic medial (mLDs) which FASN1-dependent de novo...
Abstract Mutations in the SNX14 gene cause spinocerebellar ataxia, autosomal recessive 20 (SCAR20) both humans and dogs. SCAR20 is understood to involve subcellular disruption autophagy lipid metabolism. Previously reported studies on phenotypic consequences of mutations have been limited vitro investigation patient-derived dermal fibroblasts, laboratory engineered cell lines developmental analysis zebrafish morphants. In addition, investigated biochemical roles homologues Snz ( Drosophila )...