A5057129369- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Genetics, Aging, and Longevity in Model Organisms
- Phytochemical compounds biological activities
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Cancer Cells and Metastasis
- GABA and Rice Research
- Catalysis for Biomass Conversion
- 3D Printing in Biomedical Research
- Biofuel production and bioconversion
- Cell Image Analysis Techniques
- Cancer Genomics and Diagnostics
- SARS-CoV-2 detection and testing
- Multiple Myeloma Research and Treatments
- Single-cell and spatial transcriptomics
- Protein Degradation and Inhibitors
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Biosensors and Analytical Detection
- Sirtuins and Resveratrol in Medicine
- Pancreatic function and diabetes
- Cannabis and Cannabinoid Research
- Acute Myeloid Leukemia Research
National University of Singapore
2006-2025
Yong In University
2025
National University Hospital
2006
Loma Linda University
2006
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a newly discovered lipid class known for their potential anti-inflammatory and insulin-sensitizing properties. A sustainable efficient synthesis route is essential to realize the FAHFAs enable cost-effective, large-scale production. Enzymatic synthesis, favored its scalability environmental impact, preferred approach. Candida (Moesziomyces) antarctica lipase (CalA), previously thermostability limited ability catalyze FAHFA esterification,...
Emergence of drug-resistant cancer phenotypes is a challenge for anti-cancer therapy. Cancer stem cells are identified as one the ways by which chemoresistance develops. We investigated anti-inflammatory combinatorial treatment (DA) doxorubicin and aspirin using preclinical microfluidic model on cell lines patient-derived circulating tumour clusters. The had been previously demonstrated to predict patient overall prognosis. that low-dose with sub-optimal dose 72 h could generate higher...
Polyketides are chemically diverse and medicinally important biochemicals that biosynthesized from acyl-CoA precursors by polyketide synthases. One of the limitations to combinatorial biosynthesis polyketides has been lack a toolkit describes means delivering novel necessary for biosynthesis. Using five acid-CoA ligases obtained various plants microorganisms, we an initial library 79 thioesters screening each against 123 carboxylic acids. The includes derivatives cinnamyl-CoA,...
Lipases are a group of enzymes capable catalyzing the hydrolysis triacylglycerides into free fatty acids during lipid metabolism. In presence short-chain alcohols, some these can catalyze transesterification plant oils biodiesel. Biodiesel has recently gained traction to be green alternative fossil fuel. Efficient production biodiesel at an industrial scale is limited by reusability enzyme and its tolerance toward high concentrations organic solvents. We describe scalable workflow that...
Caloric restriction (CR) is an intervention that can increase maximal lifespan in organisms, but its application to humans remains challenging. A more feasible approach achieve extension develop CR mimetics target biochemical pathways affected by CR. Recent studies the engineering and structural characterization of polyketide synthases (PKSs) have facilitated their use as biocatalysts produce novel polyketides. Here, we show establishing a combinatorial biosynthetic route Escherichia coli...
Abstract An overreliance on commercial, kit-based RNA extraction in the molecular diagnoses of infectious disease presents a challenge event supply chain disruptions and can potentially hinder testing capacity times need. In this study, we adapted well-established, robust TRIzol-based protocol into high-throughput format through miniaturization automation. The workflow was validated by RT-qPCR assay for SARS-CoV-2 detection to illustrate its scalability without interference downstream...
13064 Background: Internal tandem duplications (ITDs) of fms-like tyrosine kinase 3 (FLT3) receptor are identified in 20–25% adult AML patients associated with poor prognosis. ABT-869 is active FLT3-AML and currently under clinical investigaton. We hypothesize that the combination chemotherapy can improve therapeutic index FLT3-AML. Methods: Using Calcusyn software, additive, synergistic or antagonistic effect concurrent sequential cytosine arabinoside (Ara-C) doxorubicin (Dox) was measured...