A5073902697- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- SARS-CoV-2 detection and testing
- Gene Regulatory Network Analysis
- Microtubule and mitosis dynamics
- SARS-CoV-2 and COVID-19 Research
- Advanced Biosensing Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Advanced biosensing and bioanalysis techniques
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- Cellular transport and secretion
- Protist diversity and phylogeny
- Click Chemistry and Applications
- Luminescence and Fluorescent Materials
- Nanoplatforms for cancer theranostics
- Animal Genetics and Reproduction
- Cell Image Analysis Techniques
- Respiratory viral infections research
- Fungal and yeast genetics research
- Biosensors and Analytical Detection
- Modular Robots and Swarm Intelligence
- Infection Control and Ventilation
London Biofoundry
2019-2024
Imperial College London
2015-2024
Foundry (United Kingdom)
2019-2022
UK Dementia Research Institute
2020
Universitat Politècnica de València
2020
University of Oxford
2019-2020
Hebrew University of Jerusalem
2019
Worcester Polytechnic Institute
2019
University of Copenhagen
2019
Toscana Life Sciences
2018
Biofoundries provide an integrated infrastructure to enable the rapid design, construction, and testing of genetically reprogrammed organisms for biotechnology applications research. Many biofoundries are being built a Global Biofoundry Alliance has recently been established coordinate activities worldwide.
To understand SARS-Co-V-2 infection and transmission in UK nursing homes order to develop preventive strategies for protecting the frail elderly residents.An outbreak investigation involving 394 residents 70 staff, was carried out 4 affected by COVID-19 outbreaks central London. Two point-prevalence surveys were performed one week apart where underwent SARS-CoV-2 testing had relevant symptoms documented. Asymptomatic staff from three of four also offered testing.Overall, 26% (95% CI 22-31)...
We present a simple and environmentally friendly process for cell patterning on glass covered with an ultrathin layer of poly-L-lysine-grafted-polyethylene glycol (PLL-g-PEG) by exposure to deep UV light. The patterned substrates are stable months in the lab atmosphere before incubation proteins. Incubation proteins resulted well defined patterns, high feature resolution. RPE-1 cells seeded fibronectin/fibrinogen–Alexa 488 patterns were constrained days exposed regions. Finally, large plates...
We have developed a protocol that allows rapid and efficient purification of native, active tubulin from variety species tissue sources by affinity chromatography. The matrix comprises bacterially expressed, recombinant protein, the TOG1/2 domains Saccharomyces cerevisiae Stu2, covalently coupled to Sepharose support. resin has high capacity specifically bind clarified crude cell extracts, and, after washing, highly purified can be eluted under mild conditions. is fully functional...
The ability to quickly and reliably assemble DNA constructs is one of the key enabling technologies for synthetic biology. Here we define a new Biopart Assembly Standard Idempotent Cloning (BASIC), which exploits principle orthogonal linker based assembly physical standard parts. Further, demonstrate robust method assembly, on type IIs restriction enzyme cleavage ligation oligonucleotides with single stranded overhangs that determine order. It allows efficient, parallel great accuracy: 4...
Fluorescent reporters are commonly used to quantify activities or properties of both natural and engineered cells. Fluorescence is still typically reported only in arbitrary normalized units, however, rather than units defined using an independent calibrant, which problematic for scientific reproducibility even more so when it comes effective engineering. In this paper, we report interlaboratory study showing that simple, low-cost unit calibration protocols can remedy situation, producing...
Abstract Multi-part DNA assembly is the physical starting point for many projects in Synthetic and Molecular Biology. The ability to explore a genetic design space by building extensive libraries of constructs essential creating programmed biological systems. With multiple methods standards adopted Biology community, automation process now receiving serious attention. Automation will enable larger builds using less researcher time, while increasing accessible space. However, these benefits...
Abstract The SARS-CoV-2 pandemic has shown how a rapid rise in demand for patient and community sample testing can quickly overwhelm capability globally. With most diagnostic infrastructure dependent on specialized instruments, their exclusive reagent supplies become bottlenecks, creating an urgent need approaches to boost capacity. We address this challenge by refocusing the London Biofoundry onto development of alternative pipelines. Here, we present reagent-agnostic automated platform...
The discovery of new compounds with pharmacological properties is usually a lengthy, laborious and expensive process. Thus, there increasing interest in developing workflows that allow for the rapid synthesis evaluation libraries aim identifying leads further drug development. Herein, we apply combinatorial to build library 90 iridium(III) complexes (81 which are new) over two synthesise-and-test cycles, potential agents photodynamic therapy. We demonstrate power this approach by highly...
Background Members of the kinesin-8 subfamily are plus end-directed molecular motors that accumulate at plus-ends kinetochore-microtubules (kt-MTs) where they regulate MT dynamics. Loss vertebrate function induces hyperstable MTs and elongated mitotic spindles accompanied by severe chromosome congression defects. It has been reported motility human kinesin-8, Kif18A, is required for its accumulation tips kt-MTs. Methodology/Findings Here, we investigate how Kif18A localizes to We find...
Kinesin-1 motor proteins walk parallel to the protofilament axes of microtubules as they step from one tubulin dimer next. Is tracking an inherent property processive kinesin motors, like kinesin-1, and what are structural determinants underlying tracking? To address these questions, we investigated properties kinesin-8, Kip3. Using in vitro gliding motility assays, found that Kip3 rotates counterclockwise around their longitudinal with periodicities ∼1 μm. These rotations indicate motors...
The size and position of mitotic spindles is determined by the lengths their constituent microtubules. Regulation microtubule length requires feedback to set balance between growth shrinkage. Whereas negative mechanisms for control, based on depolymerizing kinesins severing proteins, have been studied extensively, positive are not known. Here, we report that budding yeast kinesin Kip2 a polymerase catastrophe inhibitor in vitro uses its processive motor activity as part loop further promote...
Synthetic biology research and its industrial applications rely on deterministic spatiotemporal control of gene expression. Recently, electrochemical expression has been demonstrated in electrogenetic systems (redox-responsive promoters used alongside redox inducers electrodes), allowing for the direct integration electronics with biological processes. However, use is limited by poor activity, tunability, standardization. In this work, we developed a strong, unidirectional, redox-responsive...
Abstract Nucleophilic amino acids are important in covalent drug development yet underutilized as anti-microbial targets. Chemoproteomic technologies have been developed to mine chemically accessible residues via their intrinsic reactivity towards electrophilic probes but cannot discern which reactive sites contribute protein function and should therefore be prioritized for discovery. To address this, we a CRISPR-based oligo recombineering (CORe) platform support the rapid identification,...
During the course of SARS-CoV-2 pandemic reports mutations with effects on spreading and vaccine effectiveness emerged. Large scale mutation analysis using rapid Whole Genome Sequencing (WGS) is often unavailable but could support public health organizations hospitals in monitoring transmission rising levels mutant strains. Here we report a novel WGS technique for SARS-CoV-2, EasySeq™ RC-PCR kit. By applying reverse complement polymerase chain reaction (RC-PCR), an Illumina library...
Abstract In their natural environments, microorganisms mainly operate at suboptimal growth conditions with fluctuations in nutrient abundance. The resulting cellular adaptation is subject to conflicting tasks: or survival maximisation. Here, we study this by systematically measuring the impact of a nitrogen downshift 24 sources on metabolism single-cell level. Saccharomyces lineages grown rich media and exposed gradually differentiate form two subpopulations different cell sizes where one...
CRISPR guide RNAs (gRNAs) can be programmed with relative ease to allow the genetic editing of nearly any DNA or RNA sequence. Here, we propose novel molecular architectures achieve RNA-dependent modulation activity in response specific molecules. We designed and tested, both living Escherichia coli cells cell-free assays for rapid prototyping, cis-repressed RNA-interacting (igRNA) that switch their active state only upon interaction small fragments long transcripts, including...